LOCUS       sequence-352412-        3035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Factor IX donor for twinPE and Bxb1 mediated insertion at ALB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Anzalone AV, Gao XD, Podracky CJ, Nelson AT, Koblan LW, Raguram A, 
            Levy JM, Mercer JAM, Liu DR
  TITLE     Programmable deletion, replacement, integration and inversion of 
            large DNA sequences with twin prime editing.
  JOURNAL   Nat Biotechnol. 2022 May;40(5):731-740. doi: 
            10.1038/s41587-021-01133-w. Epub 2021 Dec 9.
  PUBMED    34887556
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-021-01133-w"; journalName: "Nat Biotechnol"; date: 
            "2022-05"; volume: "40"; issue: "5"; pages: "731-740"
COMMENT     Sequence Label: Factor IX donor
FEATURES             Location/Qualifiers
     source          1..3035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..49
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    2811..3035
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aggtttgtct ggtcaaccac cgcggtctca gtggtgtacg gtacaaacct tttcttaaga
       61 gatgtaaaat tttcatgatg ttttcttttt tgctaaaact aaagaattat tcttttacat
      121 ttcagttttt cttgatcatg aaaacgccaa caaaattctg aatcggccaa agaggtataa
      181 ttcaggtaaa ttggaagagt ttgttcaagg gaaccttgag agagaatgta tggaagaaaa
      241 gtgtagtttt gaagaagcac gagaagtttt tgaaaacact gaaagaacaa ctgaattttg
      301 gaagcagtat gttgatggag atcagtgtga gtccaatcca tgtttaaatg gcggcagttg
      361 caaggatgac attaattcct atgaatgttg gtgtcccttt ggatttgaag gaaagaactg
      421 tgaattagat gtaacatgta acattaagaa tggcagatgc gagcagtttt gtaaaaatag
      481 tgctgataac aaggtggttt gctcctgtac tgagggatat cgacttgcag aaaaccagaa
      541 gtcctgtgaa ccagcagtgc catttccatg tggaagagtt tctgtttcac aaacttctaa
      601 gctcacccgt gctgagactg tttttcctga tgtggactat gtaaattcta ctgaagctga
      661 aaccattttg gataacatca ctcaaagcac ccaatcattt aatgacttca ctcgggttgt
      721 tggtggagaa gatgccaaac caggtcaatt cccttggcag gttgttttga atggtaaagt
      781 tgatgcattc tgtggaggct ctatcgttaa tgaaaaatgg attgtaactg ctgcccactg
      841 tgttgaaact ggtgttaaaa ttacagttgt cgcaggtgaa cataatattg aggagacaga
      901 acatacagag caaaagcgaa atgtgattcg aattattcct caccacaact acaatgcagc
      961 tattaataag tacaaccatg acattgccct tctggaactg gacgaaccct tagtgctaaa
     1021 cagctacgtt acacctattt gcattgctga caaggaatac acgaacatct tcctcaaatt
     1081 tggatctggc tatgtaagtg gctggggaag agtcttccac aaagggagat cagctttagt
     1141 tcttcagtac cttagagttc cacttgttga ccgagccaca tgtcttcgat ctacaaagtt
     1201 caccatctat aacaacatgt tctgtgctgg cttccatgaa ggaggtagag attcatgtca
     1261 aggagatagt gggggacccc atgttactga agtggaaggg accagtttct taactggaat
     1321 tattagctgg ggtgaagagt gtgcaatgaa aggcaaatat ggaatatata ccaaggtatc
     1381 ccggtatgtc aactggatta aggaaaaaac aaagctcact taatgaaaga tggatttcca
     1441 aggttaattc attggaattg aaaattaaca gggcctctca ctaactaatc actttcccat
     1501 cttttgttag atttgaatat atacattcta tgatcattgc tttttctctt tacaggggag
     1561 aatttcatat tttacctgag caaattgatt agaaaatgga accactagag gaatataatg
     1621 tgttaggaaa ttacagtcat ttctaagggc ccagcccttg acaaaattgt gaagttaaat
     1681 tctccactct gtccatcaga tactatggtt ctccactatg gcaactaact cactcaattt
     1741 tccctcctta gcagcattcc atcttcccga tcttctttgc ttctccaacc aaaacatcaa
     1801 tgtttattag ttctgtatac agtacaggat ctttggtcta ctctatcaca aggccagtac
     1861 cacactcatg aagaaagaac acaggagtag ctgagaggct aaaactcatc aaaaacacta
     1921 ctccttttcc tctaccctat tcctcaatct tttacctttt ccaaatccca atccccaaat
     1981 cagtttttct ctttcttact ccctctctcc cttttaccct ccatggtcgt taaaggagag
     2041 atggggagca tcattctgtt atacttctgt acacagttat acatgtctat caaacccaga
     2101 cttgcttccg tagtggagac ttgcttttca gaacataggg atgaagtaag gtgcctgaaa
     2161 agtttggggg aaaagtttct ttcagagagt taagttattt tatatatata atatatatat
     2221 aaaatatata atatacaata taaatatata gtgtgtgtgt atgcgtgtgt gtagacacac
     2281 acgcatacac acatataatg gaagcaataa gccattctaa gagcttgtat ggttatggag
     2341 gtctgactag gcatgatttc acgaaggcaa gattggcata tcattgtaac taaaaaagct
     2401 gacattgacc cagacatatt gtactctttc taaaaataat aataataatg ctaacagaaa
     2461 gaagagaacc gttcgtttgc aatctacagc tagtagagac tttgaggaag aattcaacag
     2521 tgtgtcttca gcagtgttca gagccaagca agaagttgaa gttgcctaga ccagaggaca
     2581 taagtatcat gtctccttta actagcatac cccgaagtgg agaagggtgc agcaggctca
     2641 aaggcataag tcattccaat cagccaacta agttgtcctt ttctggtttc gtgttcacca
     2701 tggaacattt tgattatagt taatccttct atcttgaatc ttctagagag ttgctgacca
     2761 actgacgtat gtttcccttt gtgaattaat aaactggtgt tctggttcat ctgtgccttc
     2821 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     2881 cactcccact gtcctttcct aataaaatga gaaaattgca tcgcattgtc tgagtaggtg
     2941 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3001 tagcaggcat gctggggatg cggtgggctc tatgg
//
