LOCUS       sequence-352425-       11113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd gen lentivector expressing shRNA from H1 promoter and GFP. For 
            direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from 
            pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLVTHM
FEATURES             Location/Qualifiers
     source          1..11113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    431..1019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             537..548
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     946..966
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1094..1804,1805..1807,1808..1810))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1094..1804,1805..1807,1808..1810))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1136..1157)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1485..1504
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1744..1765
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1807..1816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1901..2018
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2066..3244)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2075..3013
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2098..2118)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(3629..3673)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3858..4091
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4584..4709
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4756..5389
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5557..5691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5581..5600)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5635..5654
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6116..6136)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6266..6331
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7465..7794)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7479..7614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7779..7799
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(7869..7886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8040..8628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8120..8139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8799..9590,9591..9659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9422..9441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9660..9764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9832..9850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9888..9910)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10010..10029
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10243..10262
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        10552..10570
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10552..10569
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             10595..10775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    10795..10828
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(10863..11077)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(10923..10942)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    join(11106..11113,1..11,12..34,35..53,54..76,77..95,96..118,
                     119..137,138..160,161..179,180..202,203..221,222..244,
                     245..263)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
       61 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
      121 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
      181 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
      241 cttttctcta tcactgatag ggagtggtaa actcgagggg gatccactag catggtgaat
      301 tgatcccatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
      361 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctggccat
      421 acgtaccgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
      481 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
      541 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
      601 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
      661 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
      721 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
      781 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
      841 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
      901 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
      961 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta
     1021 tcatatgact agtccccgaa gcttgagctc gactagctag ctagctacta gctagtcgag
     1081 atctgagtcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     1141 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
     1201 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     1261 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     1321 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     1381 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     1441 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     1501 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     1561 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     1621 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     1681 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     1741 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     1801 tgctcaccat ggtggcgacc ggtagcgcta ggacgcgcgc gtaagcttag gccttcgaag
     1861 atcccgtagt ttaaacctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa
     1921 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     1981 ctgcactgta ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatt
     2041 aattaaattt aaatgtcgaa attcctcacg acacctgaaa tggaagaaaa aaactttgaa
     2101 ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg
     2161 agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa
     2221 actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa aagctcgaga
     2281 actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg
     2341 aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc
     2401 gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg
     2461 caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga
     2521 ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc
     2581 gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc
     2641 ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat
     2701 cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat
     2761 cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc
     2821 caggccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac
     2881 tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg
     2941 aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac
     3001 acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga
     3061 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga
     3121 catcactttc ccagtttacc ccgcgccacc ttctctaggc accggttcaa ttgccgaccc
     3181 ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg
     3241 agccaattcc cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg
     3301 tttaaaactt tatccatctt tgcaaagctt atcgtcgaca tcgcgatggt cgaggctagt
     3361 ctcgtgatcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     3421 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     3481 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     3541 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     3601 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     3661 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     3721 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     3781 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     3841 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     3901 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     3961 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     4021 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     4081 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     4141 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     4201 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     4261 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     4321 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     4381 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     4441 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     4501 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     4561 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     4621 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     4681 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     4741 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     4801 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     4861 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     4921 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct
     4981 cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc
     5041 ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg
     5101 tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc
     5161 atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct
     5221 accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg
     5281 acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc
     5341 cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt
     5401 tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca
     5461 ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag
     5521 tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt
     5581 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5641 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5701 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     5761 taaaacctct acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca
     5821 aatattcctt attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat
     5881 agttattaat agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat
     5941 tataactgtt atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata
     6001 gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa
     6061 acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct
     6121 ttctcttctt ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag
     6181 atggcatttc ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc
     6241 attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa
     6301 cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt
     6361 tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagatccgg ggcccactca
     6421 taaatccagt tgccgccacg gtagccaatc accgtatcgt ataaatcatc gtcggtacgt
     6481 tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg
     6541 cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca
     6601 agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt
     6661 tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat
     6721 gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac
     6781 gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac
     6841 gcccatatcc cacggctgtt caatccaggt atcttgcggg atatcaacaa catagtcatc
     6901 aaccagcgga cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat
     6961 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc
     7021 ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta
     7081 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc
     7141 cggtacagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt
     7201 cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg
     7261 ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata
     7321 ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg
     7381 tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg
     7441 caagcctgtc ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca
     7501 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     7561 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     7621 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     7681 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     7741 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     7801 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8461 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9781 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     9841 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     9901 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     9961 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    10021 cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct gcattaggaa
    10081 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
    10141 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
    10201 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
    10261 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
    10321 gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggt gcgggacggc
    10381 gttaccagaa actcagaagg ttcgtccaac caaaccgact ctgacggcag tttacgagag
    10441 agatgatagg gtctgcttca gtaagccaga tgctacacaa ttaggcttgt acatattgtc
    10501 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
    10561 acactataga atacacggaa ttaattctag atgctgctag agattttcca cactgactaa
    10621 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
    10681 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
    10741 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagcataact
    10801 tcgtataatg tatgctatac gaagttattc ccatgcgacg gtatcgataa gcttggagac
    10861 gcgtggtctc atacagaact tataagattc ccaaatccaa agacatttca cgtttatggt
    10921 gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc ctgtccctca
    10981 cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc
    11041 cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa ttcctgcagc cccgactttc
    11101 acttttctct atc
//
