LOCUS       sequence-352442-        8766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.HA_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        172..276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(277..345,346..1137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(495..514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1308..1896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1797..1816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2050..2067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2184..2205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2220..2225,2226..2243,2244..2250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2258..2274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2263..2285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2282..2298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2282..2298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2317..2337
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2319..2337
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2365..2591
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2592..2772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2819..2944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3437..3670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3855..3899
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4197..4445
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4197..4217
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4368..4387
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4464..4539
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4587..4704
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4844..6022
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5075..6013
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5970..5990
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6071..6080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6119..6154
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6173..6199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6209..6916
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6354..6372)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6622..6641
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6986..7574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7039..7059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7457..7468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7649..7882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7954..8075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7991..8010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8045..8064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8115..8250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8270..8289)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8271..8289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8299..8316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8299..8315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8308..8330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8457..8766,1..146)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8544..8563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(8754..8766,1..9)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
       61 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
      121 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
      181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      481 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      781 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
      901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
      961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1441 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2041 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2101 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2161 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     2221 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     2281 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     2341 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     2401 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatggcgat
     2461 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     2521 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     2581 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2641 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2701 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2761 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     2821 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     2881 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     2941 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     3001 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     3061 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     3121 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     3181 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     3241 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     3301 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3361 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3421 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3481 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3541 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3601 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     3661 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     3721 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     3781 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3841 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3901 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3961 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4021 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4081 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4141 acggatctcg acggtatcga tcacgagact agtatcgagc ggccgccccc ttcaccgagg
     4201 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     4261 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     4321 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     4381 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     4441 acaccgggtc ttcgagaaga cctgttttag agctagaaat agcaagttaa aataaggcta
     4501 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttaatt ctcgacctcg
     4561 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     4621 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4681 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     4741 ccgcggctcg aggcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     4801 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4861 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4921 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4981 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5041 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     5101 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     5161 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     5221 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     5281 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     5341 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     5401 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5461 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5521 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5581 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5641 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5701 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5761 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5821 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5881 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5941 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     6001 tcttccattt caggtgtcgt gaggaattct gcagtcgatc gacggtaccg cgggcccggg
     6061 atccacggtc gccaccatgg gcgacaccta tcggtacatt ggaggaggcg ggggagggga
     6121 ggaccaggtg gaccccaggc tgatcgacgg caagggagga ggcgggggag ggtacccata
     6181 cgatgtgcca gattacgctg gcatgctgat ggtgagcaag ggcgaggagg ataacatggc
     6241 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     6301 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     6361 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     6421 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     6481 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     6541 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     6601 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     6661 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     6721 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     6781 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     6841 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     6901 ggacgagctg tacaagaaac ggccagccga ggatatggag gaagaacaag cctttaaacg
     6961 atctcgataa agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt
     7021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7141 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7201 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7261 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7321 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7381 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7441 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7501 ggctctgcgg cctcttccgc atcttcgcct tcgccctcag acgagtcgga tctccctttg
     7561 ggccgcctcc ccgcctgggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7621 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     7681 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7741 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7801 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7861 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7921 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8041 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8101 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8221 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8281 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8341 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8401 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8641 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8701 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8761 cttgtt
//
