LOCUS       sequence-352443-        8658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains GFP21, which has a sequence similar to YFP, 
            but emission/excitation similar to GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Gu J, Tamura M, Pankov R, Danen EH, Takino T, Matsumoto K, Yamada KM
  TITLE     Shc and FAK differentially regulate cell motility and directionality
            modulated by PTEN.
  JOURNAL   J Cell Biol. 1999 Jul 26;146(2):389-403.
  PUBMED    10427092
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1999-07-26"; volume: "146(2)"; pages: "389-403"
COMMENT     Sequence Label: pGFP FAK
FEATURES             Location/Qualifiers
     source          1..8658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        53..244
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     201..221
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    313..447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(337..356)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     391..410
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      451..460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3727..3753)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(join(3772..4482,4483..4485,4486..4488))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3814..3835)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4163..4182
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4422..4443
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4485..4494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4540..4559)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(4572..4590)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4573..4590)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4800..4824)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4821..5024)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4850..4870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5025..5404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(5433..5449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5433..5449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5446..5468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5457..5473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5481..5487,5488..5505,5506..5511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5526..5547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5664..5681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5835..6423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5915..5934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6594..7385,7386..7454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7217..7236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7455..7559)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7627..7645
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7683..7705)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7805..7824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7841..8296)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7978..7999)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8190..8209
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8494..8516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8508..8525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8509..8525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(join(8545..8658,1..244))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8559..8658,1..36)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
       61 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      121 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      181 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
      241 tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg gtgctgcgcc
      301 gaattaattc ccgatccaga catgataaga tacattgatg agtttggaca aaccacaact
      361 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      421 accattataa gctgcaataa acaagttggg ccatggcggc caagctagct tctgcaggtc
      481 gactctagat gcatgctcga gcggccgctc taggatctcc caaagggcag aaagccacgt
      541 tcaatggcca ggggctcagt gtggccgtgt ctgccctagc attttcagtc ttgcttgatc
      601 aataacatca agtaggttct tggcatccac agccagggcg tgagcagctg tcagcatctg
      661 cttcttatac tcctgctgca ggctggtcat gacgtactgc tgcgccagct tcatcttgct
      721 gatgagctcg cctaagtcgg agttcagcag cttctgtgcc atctcgatct ctcgatgagt
      781 gctggctgga agagcaggaa tggtctcatc caccgtggct agtaaggtcc gcagggccag
      841 gccaacttcc ttcaccatag ggacgtactc ttctggagga gctggctgga ttttgctgga
      901 catctcgatg acagccttca ctaggcctgt cacattttca tataccttgt cattggaccg
      961 gtcaaggttg gcagtgggag gggggctgat ttcctggggc tgaagcttga caccctcatt
     1021 gtagctgtct gcagggctgc tgatgctgga caggttgctt aggtggccag gtgctccagg
     1081 gcgaggaggt ttctttggtg gagctgcagg atctggcttc cccacaggct gatagatgtg
     1141 ttggtttcca gtcggaccct gaaaactccc gtcttccctg tcgatgctgc ctcgagagag
     1201 cctcacatca ggtttcagga atcgctcttc cttctccagc cagcgctggt cttcttccat
     1261 ttcctgctgc tgtcggatta gccgctcttc catcaggtga gggggaagca cctgtcccat
     1321 ccctcgaagg tccaaagctg ccgaatcctc cacactgggc tgccacatgg acatctcctg
     1381 aggtctgtga ttccacaatt ctgtctggtc cagaagagat gcctgacctt ggtaaatgct
     1441 gccagccatg gctgggattc catgggaacc cgggtagcca gagacctggt agtgattggt
     1501 ctggaccatg tgctgtgggc tgggataaaa tccttcactg gatctcgggc taggataacc
     1561 aggtctgctg ggcttgggtg gtgcttcatc agaccctcca gagtcccagg acacggtagc
     1621 ctgtcttctg gattccatcc tcatccgttc ttcttgctgc accttctcct cctccaggat
     1681 tgtgctgagc tgagctttta gttcagtaaa cctgggccgc ctgctggggt cataggccca
     1741 acatttcgtc ataaggctgt agagggtggg aggacaattt ggaggcattg gtaatctttc
     1801 cccattttca attcgaccga tcacatcatt gttcttcact ccttgaaaag gcttcacgcc
     1861 atgcatcagt atctcccaca tacacacacc aaacatccac acgtcactag ctgaggtaaa
     1921 acgtcgaaaa ttgattgact ctggagccat ccatttaata ggtaattttc ctttggaagc
     1981 tttatagtaa gtactgtctt ccatatatcg agataatcca aagtctccca attttacaca
     2041 atcatttgaa gacaccagaa cattccgagc agcaatgtcc ctgtgaacaa atcttttgct
     2101 ctccagatac gcgagtgctg tactaagctg ataggcatat aatatcaaag atgccaggtc
     2161 caagctgtat tttcttactt gcaaaaatga cctcagctct ccaagtgtgc acaactccat
     2221 gattatccag acagggttct ctgtaatgac tccaatcagc ttcacgatgt gaggatggtc
     2281 aaactgacgc attgttaagg cttcttgaag gaacttctct ctcacgctgt ccgaagtaca
     2341 gtttttacat gttttgattg caacagccaa agctggattc tctgggctca ggtacacgcc
     2401 ttgatgtaca tctccaaact gaccttctcc aatacagcgt ccaagttcta ttctttctct
     2461 ctgaatctca taatccctgg tcgagggcat ggtgtatgtg tcttcctcat cgatgatctc
     2521 tgcatagtca tctgtctctg acacagagac tgcatgtgtc cgcatgcctt gcttttcact
     2581 gttggccaac tttggtattg atggcaacgc ccgttcacct tctttctggg gtctgatgat
     2641 gaaagattgt gtggctccat tcaccagccg gcagtatcca tcaatcaggt cggccatgtt
     2701 ctcggcaatg gttagggatg gtgccgtcac ggtcagaggc tcaggagcac cagcgatttt
     2761 gagttgtagc attcctttcc tgtctttgtc ttcactgttt gagtactgga tggtctgcac
     2821 ttggttaaag tctgctaggt gtgtgggatt gcagcctttg tctgtcaggt aactgatccc
     2881 ttcttctggg ccgattgcca attccacaga aatgatccaa cttgacccaa gggcacactt
     2941 gaagcactct ttgtcaaatc tgtacacagg agaaagaatc tcaaagaatt tcaggatgct
     3001 ttcttctcta ttaaggttgg caaactgcct gaatgtttgc tggatcagct ttcttagtgt
     3061 tttggccttg acagaatcca gtaaactctt aggaaaaaat cgctttaaac caacatcttt
     3121 ttctaatact tcatagttgg acttcttctc tagggcatta cccctcatct cccaatagga
     3181 tcgcctaatt tccaaacaac ccaacttcaa agctatttct tggtctactt gatcagctat
     3241 ttcttgcatg tagtcactct tcacctgttg atagaagaaa ttcaatgttg gcttatcttc
     3301 agtaaactgg tttagaaatc cttttggcaa gtaacgaatt ctcaactcat atttccactc
     3361 ctctggtggg tgggcgagtt catatttctc cctcacactg gagacaccca tgtccacgtg
     3421 cagccagtgc acctcctccg atcgcaggtg actgaggcga aatccatagc aggccacgtg
     3481 ctttactttg tgactgtcca ctatcttctg aatgatgccc ctgacatcag tagcatctcc
     3541 atgcctgata atactggccc aggtggttgg ctcactactg ctttcaaaat gatgaaagac
     3601 ctttaatact cgttccattg caccagggga ccgttccatg ccagtaccca ggtgagtctt
     3661 agtactcgaa cttggtgtgt gattcaagtt tgggtcaaga taagcagctg ccataccgga
     3721 tctcgggagg ctagcgtaat caggaacgtc gtacatggtg gatccgcctc ccttgtacag
     3781 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     3841 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     3901 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     3961 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     4021 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     4081 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     4141 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     4201 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     4261 ggggtagcgg gcgaagcact gcaggccgta ggcgaaggtg gtcacgaggg tgggccaggg
     4321 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     4381 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     4441 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcggctga
     4501 atcgatagaa ccgaggtgca gttggacctg ggagtggaca cctgtggaga gaaaggcaaa
     4561 gtggatgtta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     4621 taattgtagc cgcgttctaa cgaagccaag ggggtgggcc tatagactct ataggcggta
     4681 cttacgtcac tcttggcacg gggaatccgc gttccaatgc accgttcccg gccgcggagg
     4741 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
     4801 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
     4861 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     4921 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     4981 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     5041 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     5101 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     5161 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     5221 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     5281 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     5341 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     5401 tgtcgggcga gctcgaatta attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     5461 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5521 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6241 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7561 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7621 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7681 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7741 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7801 tatgcggcat cagagcagat tgtactgaga gtgcaccata aaattgtaaa cgttaatatt
     7861 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     7921 atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca
     7981 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8041 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
     8101 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8161 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     8221 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     8281 ccgctacagg gcgcgtacta tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat
     8341 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     8401 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     8461 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     8521 ccagtgccaa gctgttaaca gctttttgca aaagcctagg cctccaaaaa agcctcctca
     8581 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     8641 tagtcagcca tggggcgg
//
