LOCUS       sequence-352454-       13043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial lactate 2-monooxygenase (LMO) enzyme in 
            astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Vaccari Cardoso B, Barrera I, Mosienko V, Gourine AV, Kasparov S, 
            Teschemacher AG
  TITLE     Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate 
            Lactate Release.
  JOURNAL   Brain Sci. 2021 Aug 10;11(8). pii: brainsci11081056. doi: 
            10.3390/brainsci11081056.
  PUBMED    34439675
REFERENCE   2  (bases 1 to 13043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain 
            Sci."; date: "2021-08-10"; volume: "11(8). pii: brainsci11081056. 
            doi"; pages: " 10.3390/brainsci11081056"
COMMENT     Sequence Label: pXCX-sGFAP-LMO-IRES-tdTomato
FEATURES             Location/Qualifiers
     source          1..13043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..1440
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(1528..1548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1946..1957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2089..2106)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2095..2319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2699..2800
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(2822..2840)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2908..3012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3013..3081,3082..3873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4044..4632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4533..4552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4786..4803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4818..4958
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4884..4903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5044..5066
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5060..5251)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     polyA_signal    9072..9193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9083..9102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9137..9156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9436..9978)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(10042..10482)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(10519..10537)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(10583..10621)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ccggtcgcca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
       61 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      121 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
      181 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
      241 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      301 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
      361 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
      421 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
      481 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
      541 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
      601 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
      661 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
      721 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
      781 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
      841 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      901 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
      961 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     1021 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     1081 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     1141 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     1201 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     1261 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     1321 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1381 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtag
     1441 gcggccgccc cgttatttgc actctgttcc tgttaatcaa cctctggatt acaaaatttg
     1501 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1561 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1621 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1681 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1741 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1801 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     1861 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     1921 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     1981 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2041 ctccctttgg gccgcctatc gattagggat aacagggtaa tggatcagcc tcgactgtgc
     2101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2281 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2341 gctagagctg actataataa taaaacgcca actttgaccc ggaacgcgga aaacacctga
     2401 gaaaaacacc tgggcgagtc tccacgtaaa cggtcaaagt ccccgcggcc ctagacaaat
     2461 attacgcgct atgagtaaca cagaattatt cagatttcac ttcctcttat tcagttttcc
     2521 cgcgaaaatg gccaaatatt acttggttac gcccaaattt actacaacat ccgcctaaaa
     2581 ccgcgcgaaa attgtcactt cccgtataca ccggcgcaca ccaaaaacgt cacttttacc
     2641 acatccggcg cttacatgtg ttccgccaca cttgcaacat cacacttccg ccacactact
     2701 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
     2761 atattggctt caatccaaaa taaggtatat tatgatgatg ggaattcttg aagacgaaag
     2821 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3241 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3541 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3721 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3961 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4801 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4861 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     4921 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     4981 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5041 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     5101 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     5161 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     5221 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     5281 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
     5341 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
     5401 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
     5461 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     5521 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     5581 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     5641 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     5701 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     5761 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
     5821 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
     5881 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     5941 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     6001 ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc
     6061 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     6121 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     6181 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat
     6241 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     6301 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     6361 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
     6421 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
     6481 gactgggttg aaggctctca agggcatcgg tcgaggacag gcctctcaag tctgtatacg
     6541 gggacacgga cagccttttc gtcaccgagc gtggacaccg gctcatggaa accagaggta
     6601 agaaacgcat caaaaagcat gggggaaacc tggtttttga ccccgaacgg ccagagctca
     6661 cctggctcgt ggaatgcgag accgtctgcg gggcctgcgg cgcggatgcc tactccccgg
     6721 aatcggtatt tctcgcgccc aagctctacg ccctcaaaag tctgcactgc ccctcgtgcg
     6781 gcgcctcctc caagggcaag ctgcgcgcca agggccacgc cgcggagggg ctggactatg
     6841 acaccatggt caaatgctac ctggccgacg cgcagggcga agaccggcag cgcttcagca
     6901 ccagcaggac cagcctcaag cgcaccctgg ccagcgcgca gcccggagcg caccccttca
     6961 ccgtgaccca gactacgctg acgaggaccc tgcgcccgtg gaaagacatg accctggccc
     7021 gtctggacga gcaccgacta ctgccgtaca gcgaaagccg ccccaacccg cgaaacgagg
     7081 agatatgctg gatcgagatg ccgtagagca ggtgaccgag ctgtgggacc gcctggaact
     7141 gcttggtcaa acgctcaaaa gcatgcctac ggcggacggt ctcaaaccgt tgaaaaactt
     7201 tgcttccttg caagaactgc tatcgctggg cggcgagcgc cttctggcgg atttggtcag
     7261 ggaaaacatg cgagtcaggg acatgcttaa cgaagtggcc cccctgctca gggatgacgg
     7321 cagctgcagc tctcttaact accagttgca gccggtaata ggtgtgattt acgggcccac
     7381 cggctgcggt aagtcgcagc tgctcaggaa cctgctttct tcccagctga tctcccctac
     7441 cccggaaacc gttttcttca tcgccccgca ggtagacatg atccccccat ctgaactcaa
     7501 agcgtgggaa atgcaaatct gtgagggtaa ctacgcccct gggccggatg gaaccattat
     7561 accgcagtct ggcaccctcc gcccgcgctt tgtaaaaatg gcctatgacg atctcatcct
     7621 ggaacacaac tatgacgtta gtgatcccag aaatatcttc gcccaggccg ccgcccgtgg
     7681 gcccattgcc atcattatgg acgaatgcat ggaaaatctt ggaggtcaca agggcgtctc
     7741 caagttcttc cacgcatttc cttctaagct acatgacaaa tttcccaagt gcaccggata
     7801 cactgtgctg gtggttctgc acaacatgaa tccccggagg gatatggctg ggaacatagc
     7861 caacctaaaa atacagtcca agatgcatct catatcccca cgtatgcacc catcccagct
     7921 taaccgcttt gtaaacactt acaccaaggg cctgcccctg gcaatcagct tgctactgaa
     7981 agacattttt aggcaccacg cccagcgctc ctgctacgac tggatcatct acaacaccac
     8041 cccgcagcat gaagctctgc agtggtgcta cctccacccc agagacgggc ttatgcccat
     8101 gtatctgaac atccagagtc acctttacca cgtcctggaa aaaatacaca ggaccctcaa
     8161 cgaccgagac cgctggtccc gggcctaccg cgcgcgcaaa acccctaaat aaagacagca
     8221 agacacttgc ttgatccaaa tccaaacaga gtctggtttt ttatttatgt tttaaaccgc
     8281 attgggaggg gaggaagcct tcagggcaga aacctgctgg cgcagatcca acagctgctg
     8341 agaaacgaca ttaagttccc gggtcaaaga atccaattgt gccaaaagag ccgtcaactt
     8401 gtcatcgcgg gcggatgaac gggaagctgc actgcttgca agcgggctca ggaaagcaaa
     8461 gtcagtcaca atcccgcggg cggtggctgc agcggctgaa gcggcggcgg aggctgcagt
     8521 ctccaacggc gttccagaca cggtctcgta ggtcaaggta gtagagtttg cgggcaggac
     8581 ggggcgacca tcaatgctgg agcccatcac attctgacgc accccggccc atgggggcat
     8641 gcgcgttgtc aaatatgagc tcacaatgct tccatcaaac gagttggtgc tcatggcggc
     8701 ggcggctgct gcaaaacaga tacaaaacta cataagaccc ccaccttata tattctttcc
     8761 cacccttaag ccacgcccac acatttcagt acctcaatct gtatcttcat cgctagagcc
     8821 aaactcagcg cgggtgcagg ccagcaccaa gtgatcgggc ctcagctcct cggtcacatc
     8881 cagcatcaca ggctggttcc taatatgttt accgccacac tcgcagggtc tgcacctggt
     8941 gcgggtctca tcgtacctca gcaccttcca ctctagcgat gtacgggcca gatatacgcg
     9001 atagggataa cagggtaatg atatctctga ccgctagcat cgtcgacccg ggatccgcgg
     9061 actagtcgcg ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     9121 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     9181 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     9241 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     9301 tgaacagact gtgaggactg aggggcctga aatgagcctt gggactgtga atctaaaata
     9361 cacaaacaat tagaatcagt agtttaacac attatacact taaaaattgg atctacttat
     9421 tcaagatcgg ccgctggagc tgatctgact cagcagagca gagaagtcca tgtccgcaat
     9481 ggaggagaag tcttcatctc cggagagacc attgggaagc cccgaggtcc ccaggggtgt
     9541 gggagctggg tcagggggcc tctgggaccc tgtcaccagg cgagttatag cttcagggta
     9601 ctccatcagc atgggctcag ctgtggagtg agacatggac acaccctggt tcaggagctg
     9661 ctgaaactct gagttgtcca cagatgccag gtctgtgaac actcctgggt ctgtgctgtt
     9721 gccaagcaag gcccccaagt cttcatcagc atcaaactgc aggtgcagca gggcttccga
     9781 cagcgtgcct tccccagcct gggtgctctt tggaacaggt gcagacaggg actggggagg
     9841 acccggggtt agaactgggg caggagctgg gggctgagcc agaggtacca tggctgagga
     9901 agggaccatg gtctgggcaa ggactggggc agaggacggt gctaaggcca gggcctggtt
     9961 tgagatctgc ccagaaggaa acaccatggt gagctctgca gaagcttcta gaattccagc
    10021 ccgggcggat cccaattccg gcgatacagt caactgtctt tgacctttgt tactactctc
    10081 ttccgatgat gatgtcgcac ttattctatg ctgtctcaat gttagaggca tatcagtctc
    10141 cactgaagcc aatctatctg tgacggcatc tttattcaca ttatcttgta caaataatcc
    10201 tgttaacaat gcttttatat cctgtaaaga atccattttc aaaatcatgt caaggtcttc
    10261 tcgaggaaaa atcagtagaa atagctgttc cagtctttct agccttgatt ccacttctgt
    10321 cagatgtgcc ctagtcagcg gagacctttt ggttttggga gagtagcgac actcccagtt
    10381 gttcttcaga cacttggcgc acttcggttt ttctttggag cacttgagct ttttaagtcg
    10441 gcaaatatcg catgcttgtt cgatagaaga cagtagcttc atctttcagg aggctagctt
    10501 agatcccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcgctagc
    10561 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctaa
    10621 cgcgctagcc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
    10681 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagagggac
    10741 gcgtaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc gggggcctga
    10801 gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa gcagacctgg
    10861 cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat gactcacctt
    10921 ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac cccagccccc
    10981 ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc cagcctgaca
    11041 gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg ctgtgtggcg
    11101 ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg gatcagggga
    11161 tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc ttcccagaag
    11221 tccaaggaca caaatgggtg aggggagagc tctccccata gctgggctgc ggcccaaccc
    11281 caccccctca ggctatgcca gggggtgttg ccaggggcac ccgggcatcg ccagtctagc
    11341 ccactccttc ataaagccct cgcatcccag gagcgagcag agccagagca ggttggagag
    11401 gagacgcatc acctccgctg ctcgcactag cgctaccgga ctcagatctc gagctcaagc
    11461 ttcgaagccg ccaccatgag caactggggc gactacgaga acgaaatcta cggccaggga
    11521 ctcgtgggag tcgctcctac actgcctatg agctacgccg actgggaagc tcatgctcag
    11581 caagcacttc cacctggcgt gctgtcttat gtggctggcg gaagcggaga tgagcacaca
    11641 cagagggcta acgtggaagc cttcaagcac tggggcctga tgcctagaat gctgatggct
    11701 gccaccgaga gggacctgtc tgttgagctg tggggcaaga catgggccgc tcctatgttc
    11761 ttcgctccca tcggagtgat cgccctgtgt gcccaagatg gacatggcga cgctgcatct
    11821 gctcaggcct ctgctagaac cggcgtgccc tacatcacaa gcacactggc tgtgtccagc
    11881 ctcgaggaca tcagaaagca cgctggcgat acccctgcct acttccagct gtactacccc
    11941 gaggatcgcg acctggccga gagctttatc agaagggctg aagaggccgg ctacgacggc
    12001 ctggttatca cactggacac ctggatcttc ggctggcggc ctagggatct gacaatcagc
    12061 aacttcccat tcctgagagg cctgtgcctg accaactacg tgacagaccc cgtgttccag
    12121 aagaagttca aggcccacag cggcgtggaa gctgagggcc tgagagataa ccctagactg
    12181 gccgccgatt tctggcacgg cctgtttgga cactctgtga cctgggaaga tattgactgg
    12241 gtccgatcca tcaccaagat gcccgtgatc ctgaagggca tccagcatcc tgacgatgct
    12301 cggagagctg tggatagcgg agtggacggc atctactgtt ctaaccacgg cggcagacag
    12361 gccaatggtg gactgcctgc tctggactgt ctgcccgaag tggttaaggc cagcggcgat
    12421 acacctgtgc tgttcgacag cggcatcaga acaggcgccg atgtggtcaa ggctctggct
    12481 atgggagctt ctgctgtcgg catcggcaga ccttatgctt ggggagctgc tctcggcggc
    12541 tctaagggaa tcgagcatgt ggctagaagc ctgctggccg aggccgatct gatcatggct
    12601 gtggacggct acagaaacct gaaagagctg accatcgacg ccctgaggcc taccagataa
    12661 gatatctgca gtcgacggta ccagatgggg gtcctgggcc ccagggtgtg cagccactga
    12721 cttggggact gctggtgggg tagggatgag ggagggaggg gcattgtgat gtacagggct
    12781 gctctgtgag atcaagggtc tcttaagggt gggagctggg gcagggacta cgagagcagc
    12841 cagatgggct gaaagtggaa ctcaaggggt ttctggcacc tacctacctg cttcccgctg
    12901 gggggtgggg agttggccca gagtcttaag attggggcag ggtggagagg tgggctcttc
    12961 ctgcttccca ctcatcttat agctttcttt ccccagatcc gaattcgaga tccaaaccaa
    13021 gggtaccgcg ggcccgggat cca
//
