LOCUS       sequence-352462-       17833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV dual reporter vector expressing mCh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17833)
  AUTHORS   Lassen KG, Hebbeler AM, Bhattacharyya D, Lobritz MA, Greene WC
  TITLE     A flexible model of HIV-1 latency permitting evaluation of many 
            primary CD4 T-cell reservoirs.
  JOURNAL   PLoS One. 2012;7(1):e30176. doi: 10.1371/journal.pone.0030176. Epub 
            2012 Jan 24.
  PUBMED    22291913
REFERENCE   2  (bases 1 to 17833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1):e30176. doi"; pages: " 
            10.1371/journal.pone.0030176. Epub 2012 Jan 24"
COMMENT     Sequence Label: NL4-3 mCherry Luciferase
FEATURES             Location/Qualifiers
     source          1..17833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             159..792
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(1082..1090,1091..1093,1094..1664)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1252..1271)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1481..1498
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1669..3318)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     3237..3255
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(3322..3375)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3382..4089)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEGYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3657..3676)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3926..3944
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4335..4361)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(4655..4699)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4884..5117
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             complement(7780..10791)
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    7978..8095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10584..12086)
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     LTR             12242..12499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(14192..14211)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14311..14333
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(14371..14389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14457..14561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14562..14630,14631..15422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14780..14799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15593..16181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16082..16101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16335..16352
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actgcgccca gcccaaatat atatattttt atgacctcca tgtataaata tatttaatac
       61 ccttaactta ttattattat tttagacagt cttgttttct tgcccaggct ggaatgcagt
      121 ggcgcgatct tggctcactg caacctctac ctcctgggtg ctagagattt tccacactga
      181 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
      241 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
      301 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
      361 cttatatgca gcatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
      421 gaaagtcccc agcggaaagt cccttgtagc aagctcgatg tcagcagttc ttgaagtact
      481 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
      541 acacttctct ctcagggtca tccattccat gcaggctcac agggtgtaac aagctggtgt
      601 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
      661 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc caatcaggga
      721 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttctt tgggagtgaa
      781 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
      841 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctct tcctcctctt
      901 gtgcttctag ccaggcacaa gcagcattgt tagctgctgt attgctactt gtgattgctc
      961 catgtttttc taggtctcga catactgctc ccaccccatc tgctgctggc tcagctcgtc
     1021 tcattctttc ccttacagca ggccatccaa tcacactact ttttgaccac ttgccaccca
     1081 tggttgtggc cattatcatc gtgtttttca aaggaaaacc acgtccccgt ggttcggggg
     1141 gcctagacgt tttttaacct cgactaaaca catgtaaagc atgtgcaccg aggccccaga
     1201 tcagatccca tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt
     1261 gaggagagcc atttgactct ttccacaact atccaactca caacgtggca ctggggttgt
     1321 gccgcctttg caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca
     1381 ggtggggggt tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct
     1441 tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg
     1501 gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg
     1561 ccaaaagacg gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc
     1621 aagcggcttc ggccagtaac gttagggggg gggagggaga ggggctcctt acacggcgat
     1681 ctttccgccc ttcttggcct ttatgaggat ctctctgatt tttcttgcgt cgagttttcc
     1741 ggtaagacct ttcggtactt cgtccacaaa cacaactcct ccgcgcaact ttttcgcggt
     1801 tgttacttga ctggcgacgt aatccacgat ctctttttcc gtcatcgtct ttccgtgctc
     1861 caaaacaaca acggcggcgg gaagttcacc ggcgtcatcg tcgggaagac ctgcgacacc
     1921 tgcgtcgaag atgttggggt gttggagcaa gatggattcc aattcagcgg gagccacctg
     1981 atagcctttg tacttaatca gagacttcag gcggtcaacg atgaagaagt gttcgtcttc
     2041 gtcccagtaa gctatgtctc cagaatgtag ccatccatcc ttgtcaatca aggcgttggt
     2101 cgcttccgga ttgtttacat aaccggacat aatcatagga cctctcacac acagttcgcc
     2161 tctttgatta acgcccagcg ttttcccggt atccagatcc acaaccttcg cttcaaaaaa
     2221 tggaacaact ttaccgaccg cgcccggttt atcatccccc tcgggcgtaa tcagaatagc
     2281 tgatgtagtc tcagtgagcc catatccttg cctgatacct ggcagatgga acctcttggc
     2341 aaccgcttcc ccgacttcct tagagagggg agcgccacca gaagcaattt cgtgtaaatt
     2401 agataaatcg tatttgtcaa tcagagtgct tttggcgaag aaggagaata gggttggcac
     2461 cagcagcgca ctttgaatct tgtaatcctg aaggctcctc agaaacagct cttcttcaaa
     2521 tctatacatt aagacgactc gaaatccaca tatcaaatat ccgagtgtag taaacattcc
     2581 aaaaccgtga tggaatggaa caacacttaa aatcgcagta tccggaatga tttgattgcc
     2641 aaaaatagga tctctggcat gcgagaatct cacgcaggca gttctatgag gcagagcgac
     2701 acctttaggc agaccagtag atccagagga gttcatgatc agtgcaattg tcttgtccct
     2761 atcgaaggac tctggcacaa aatcgtattc attaaaaccg ggaggtagat gagatgtgac
     2821 gaacgtgtac atcgactgaa atccctggta atccgtttta gaatccatga taataatttt
     2881 ttggatgatt gggagctttt tttgcacgtt caaaattttt tgcaacccct ttttggaaac
     2941 gaacaccacg gtaggctgcg aaatgcccat actgttgagc aattcacgtt cattataaat
     3001 gtcgttcgcg ggcgcaactg caactccgat aaataacgcg cccaacaccg gcataaagaa
     3061 ttgaagagag ttttcactgc atacgacgat tctgtgattt gtattcagcc catatcgttt
     3121 catagcttct gccaaccgaa cggacatttc gaagtactca gcgtaagtga tgtccacctc
     3181 gatatgtgca tctgtaaaag caattgttcc aggaaccagg gcgtatctct tcatagcctt
     3241 atgcagttgc tctccagcgg ttccatcttc cagcggatag aatggcgccg ggcctttctt
     3301 tatgtttttg gcgtcttctc gagggccggg attctcctcc acgtcaccgc atgttagaag
     3361 acttcctctg ccctcccggg gcttgtacag ctcgtccatg ccgccggtgg agtggcggcc
     3421 ctcggcgcgt tcgtactgtt ccacgatggt gtagccctcg ttgtgggagg tgatgtccaa
     3481 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
     3541 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
     3601 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
     3661 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
     3721 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
     3781 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
     3841 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     3901 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
     3961 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     4021 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
     4081 gctcaccatc ttatagcaaa atcctttcca agccctgtct tattcttcta ggtatgtggc
     4141 gaatagctct ataagctgct tgtaatactt ctataaccct atctgtcccc tcagctactg
     4201 ctatggctgt ggcattgagc aagttaacag cactattctt tagttcctga ctccaatact
     4261 gtaggagatt ccaccaatat ttgagggctt cccaccccct gcgtcccaga agttccacaa
     4321 tcctcgttac aatcaagagt aagtctctca agcggtggta gctgaagagg cacaggctcc
     4381 gcagatcgtc ccagataagt gctaaggatc cgttcactaa tcgaatggat ctgtctctgt
     4441 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc gggattggga
     4501 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
     4561 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
     4621 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
     4681 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
     4741 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcatgtta ttccaaatct
     4801 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
     4861 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
     4921 ggattcttgc ctggagctgt ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     4981 cctcaatagc cctcagcaaa ttgttctgct gctgcactat atcagacaat aattgtctgg
     5041 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     5101 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     5161 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     5221 ttctccaatt gtccctcata tcgcctcctc caggtctgaa gatctcggac ccattgttgt
     5281 tattaccacc atctcttgtt aatagcagcc cagtaatatt tgatgaacat ctaatttgtc
     5341 cactgatggg aggggcatac attgcttttc ctacttcctg ccacatgttt ataaattgtt
     5401 ttattctgca tgggagtgtg attgtgtcac ttccttcagt gttatttgac ccttcagtac
     5461 tccaagtact attaaaccaa gtactattaa acagttgtgt tgaattacag tagaaaaatt
     5521 cccctccaca attaaaactg tgcgttacaa tttctgggtc ccctcctgag gattgcttaa
     5581 agattattgt tttattattt ccaaattgtt ctcttaattt gctagctatc tgttttaaag
     5641 tggcattcca ttttgctcta ctaatgttac aatgtgcttg tctcatattt cctatttttc
     5701 ctattgtaac aaatgctctc cctggtcccc tctggatacg gatacttttt cttgtattgt
     5761 tgttgggtct tgtacaatta atttctacag atgtgttcag ctgtactatt atggttttag
     5821 cattgtctgt gaaattggca gatctaatta ctacatcttc ttctgctaga ctgccattta
     5881 acagcagttg agttgatact actggcctga ttccatgtgt acattgtact gtgctgacat
     5941 ttgtacatgg tcctgttcca ttgaacgtct tattattaca ttttagaatc gcaaaaccag
     6001 ccggggcaca ataatgtatg ggaattggct caaaggatac ctttggacag gcctgtgtaa
     6061 tgactgaggt gttacaactt atcaacctat agctggtatt atctattggt actatatcaa
     6121 gtttataaaa gaatgcatat tctttctgca ccttatctct tatgcttgtg ctgatattga
     6181 aagagcagtt ttttatctct cctttctcca ttatcattct cccgctacta ctattggtat
     6241 tagtatcatt cttcaaatca gtgcacttta aactaacaca gagtggggtt aattttacac
     6301 atggctttag gctttgatcc cataaactga ttatatcctc atgcatctgt tctaccatgt
     6361 catttttcca catgttaaaa ttttctgtca catttaccaa tactacttct tgtgggttgg
     6421 ggtctgtggg tacacaggca tgtgtggccc aaacattatg tacctctgta tcatatgctt
     6481 tagcatctga tgcacaaaat agagtggtgg ttgcttcctt ccacacaggt accccataat
     6541 agactgtgac ccacaatttt tctgtagcac tacagatcat caatatccca aggagcatgg
     6601 tgccccattt ccacccccat ctccacaagt gctgatactt ctccttcact ctcattgcca
     6661 ctgtcttctg ctctttctat tagtctatca attaacctgt ctatttttct ttgtcttaat
     6721 attttcctat attctatgat tactatggac cacacaacta ttgctattat tattgctact
     6781 actaatgcta ctattgctac tattataggt tgcattacat gtactactta ctgctttgat
     6841 agagaagctt gatgagtctg actgttctga tgagctcttc gtcgctgtct ccgcttcttc
     6901 ctgccatagg agatgcctaa ggcttttgtc atgaaacaaa cttggcaatg aaagcaacac
     6961 tttttacaat agcaattggt acaagcagtt ttaggctgac ttcctggatg cttccagggc
     7021 tctagtctag gatctactgg ctccatttct tgctctcctc tgtcgagtaa cgcctattct
     7081 gctatgtcga cacccaattc tgaaatggat aaacagcagt tgttgcagaa ttcttattat
     7141 ggcttccact cctgcccaag tatccccgta agtttcatag atatgttgtc ctaagttatg
     7201 gagccatatc ctaggaaaat gtctaacagc ttcactctta agttcctcta aaagctctag
     7261 tgtccattca ttgtatggct ccctctgtgg cccttggtct tctggggctt gttccatctg
     7321 tcctctgtca gtttcctaac actaggcaaa ggtggcttta tctgttttgg ttttattaat
     7381 gctgctagtg ccaagtactg tagagatcct accttgttat gtcctgcttg atattcacac
     7441 ctaggactaa ctatacgtcc taatatggta tttcttatag cagattctga aaaacaatca
     7501 aaatagtgca gatgaattag ttggtctgct aggtcagggt ctacttgtgt gctatatctc
     7561 tttttcctcc attctatgga gactccctga cccaaatgcc agtctctttc tcctgtatgc
     7621 agaccccaat atgttgttat tactaattta gcatccccta gtgggatgtg tacttctgaa
     7681 cttatttttg gattagtact ttcatagtga tgtctataaa accagtcctt agctttcctt
     7741 gaaatataca tatggtgttt tactaatctt ttccatgtgt taatcctcat cctgtctact
     7801 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctgatgatct ttgcttttct
     7861 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     7921 gaggagcttt gctggtcctt tccaaactgg atctctgctg tccctgtaat aaacccgaaa
     7981 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     8041 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     8101 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     8161 tataattttc tttaattctt tattcataga ttctattact ccttgacttt ggggattgta
     8221 gggaatgcca aattcctgct tgatccccgc ccaccaacag gcggccttaa ctgtagtact
     8281 ggtgaaattg ctgccattgt ctgtatgtac tgtttttact ggccatcttc ctgctaattt
     8341 taagaggaag tatgctgttt cttgccctgt ctctgctgga attacttctg cttctatata
     8401 tccactggct acatgaactg ctaccaagat aacttttcct tctaaatgtg tacaatctag
     8461 ctgccatatt cctgggctac agtctacttg tccatgcatg gcttcccctt ttagctgaca
     8521 tttatcacag ctggctacta tttcttttgc tactacaggt ggtaggttaa aatcactagc
     8581 cattgctctc caattactgt gatatttctc atgttcttct tgggccttat ctattccatc
     8641 taaaaatagt actttcctga ttccagcact gaccaattta tctacttgtt catttcctcc
     8701 aattcctttg tgtgctggta cccatgccag gtagactttt tcctttttta ttaactgctc
     8761 tattatttga ctgactaact ctgattcact cttatctggt tgtgcttgaa tgattcccaa
     8821 tgcatattgt gagtctgtca ctatgtttac ttctaatccc gaatcctgca aagctagatg
     8881 aattgcttgt aactcagtct tctgatttgt tgtgtccgtt agggggacaa ctttttgtct
     8941 tcctctgtca gttacatatc ctgcttttcc taatttagtt tccctattgg ctgccccatc
     9001 tacatagaaa gtttctgctc ctattatggg ttctttctct aactggtacc ataacttcac
     9061 taagggaggg gtattgacaa actcccactc aggaatccag gtggcttgcc aatactctgt
     9121 ccaccatgct tcccatgttt ccttttgtat gggtaattta aatttaggag tctttcccca
     9181 tattactatg ctttctgtgg ctattttttg tactgcctct gttaattgtt tcacatcatt
     9241 agtgtgggca cccttcattc ttgcatactt tcctgttttc agatttttaa atggctcttg
     9301 ataaatttga tatgtccatt ggccttgccc ctgcttctgt atttctgcta ttaagtcttt
     9361 tgatgggtca taatacactc catgtaccgg ttcttttaga atctccctgt tttctgccag
     9421 ttctagctct gcttcttctg ttagtggtac tacttctgtt agtgctttgg ttcccctaag
     9481 aagtttacat aattgcctta ctttaatccc tgcataaatc tgacttgccc aattcaattt
     9541 tcccactaat ttctgtatgt cattgacagt ccagctgtcc ttttctggca gcactatagg
     9601 ctgtactgtc catttatcag gatggagttc ataacccatc caaaggaatg gaggttcttt
     9661 ctgatgtttt ttgtctggtg tggtaaatcc ccacctcaac agatgttgtc tcagttcctc
     9721 tatttttgtt ctatgctgcc ctatttctaa gtcagatcct acatacaaat catccatgta
     9781 ttgatagatg actatgtctg gattttgttt tctaaaaggc tctaagattt ttgtcatgct
     9841 acactggaat attgctggtg atcctttcca tccctgtgga agcacattgt actgatatct
     9901 aatccctggt gtctcattgt ttatactagg tatggtaaat gcagtatact tcctgaagtc
     9961 tttatctaag ggaactgaaa aatatgcatc gcccacatcc agtactgtta ctgatttttt
    10021 ctgttttaac cctgcaggat gtggtattcc taattgaact tcccagaaat cttgagttct
    10081 cttattaagt tctctgaaat ctactaattt tctccattta gtactgtctt ttttctttat
    10141 ggcaaatact ggagtattgt atggattttc aggcccaatt tttgaaattt ttccttcctt
    10201 ttccatttct gtacaaattt ctactaatgc ttttattttt tcttctgtca atggccattg
    10261 tttaactttt gggccatcca ttcctggctt taattttact ggtacagtct caataggact
    10321 aatgggaaaa tttaaagtgc agccaatctg agtcaacaga tttcttccaa ttatgttgac
    10381 aggtgtaggt cctactaata ctgtacctat agctttatgt ccgcagattt ctatgagtat
    10441 ctgatcatac tgtcttactt tgataaaacc tccaattccc cctatcattt ttggtttcca
    10501 tcttcctggc aaattcattt cttctaatac tgtatcatct gctcctgtat ctaatagagc
    10561 ttcctttaat tgccccccta tctttattgt gacgaggggt cgctgccaaa gagtgatctg
    10621 agggaagcta aaggatacag ttccttgtct atcggctcct gcttctgaga gggagttgtt
    10681 gtctcttccc caaacctgaa gctctcttct ggtggggctg ttggctctgg tctgctctga
    10741 agaaaattcc ctggccttcc cttgtgggaa ggccagatct tccctaaaaa attagcctgt
    10801 ctctcagtac aatctttcat ttggtgtcct tcctttccac atttccaaca gccctttttc
    10861 ctaggggccc tgcaattttt ggctatgtgc ccttctttgc cacaattgaa acacttaaca
    10921 gtctttcttt ggttcctaaa attgcctttc tgtatcatta tggtagctgg atttgttact
    10981 tggctcattg cttcagccaa aactcttgct ttatggccgg gtccccccac tccctgacat
    11041 gctgtcatca tttcttctag tgtcgctcct ggtcccaatg cttttaaaat agtcttacaa
    11101 tctgggttcg cattttggac caacaaggtt tctgtcatcc aattttttac ctcttgtgaa
    11161 gcttgctcgg ctcttagagt tttatagaat cggtctacat agtctctaaa gggttccttt
    11221 ggtccttgtc ttatgtccag aatgctggta gggctataca ttcttactat tttatttaat
    11281 cccaggatta tccatctttt atagatttct cctactggga taggtggatt atgtgtcatc
    11341 catcctattt gttcctgaag ggtactagta gttcctgcta tgtcacttcc ccttggttct
    11401 ctcatctggc ctggtgcaat aggccctgca tgcactggat gcaatctatc ccattctgca
    11461 gcttcctcat tgatggtctc ttttaacatt tgcatggctg cttgatgtcc ccccactgtg
    11521 tttagcatgg tatttaaatc ttgtggggtg gctccttctg ataatgctga aaacatgggt
    11581 attacttctg ggctgaaagc cttctcttct actactttta cccatgcatt taaagttcta
    11641 ggtgatatgg cctgatgtac catttgcccc tggaggttct gcactatagg gtaattttgg
    11701 ctgacctggc tgttgtttcc tgtgtcagct gctgcttgct gtgccttttt cttacttttg
    11761 ttttgctctt cctctatctt atctaaggct tccttggtgt cttttacatc tatcctttga
    11821 tgcacacaat agaggactgc tattgtatta tataatgatc taagttcttc tgatcctgtc
    11881 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ctctaaaagg
    11941 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttagttta
    12001 tattgtttct ttccccctgg ccttaaccga attttttccc atttatctaa ttctcccccg
    12061 cttaataccg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
    12121 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
    12181 cctgcgtcga gagatctcct ctggctttac tttcgctttc aagtccctgt tcgggcgcca
    12241 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
    12301 cacaacagac gggcacacac tactttgagc actcaaggca agctttattg aggcttaagc
    12361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
    12421 ccagtacagg caaaaagcag ctgcttatat gtagcatctg agggctcgcc actccccagt
    12481 cccgcccagg ccacacctcc ctggaaagtc cccagcggaa agtcccttgt agaaagctcg
    12541 atgtcagcag tctttgtagt actccggatg cagctctcgg gccatgtgac gaaatgctag
    12601 gaggctgtca aacttccaca ctaatacttc tccctccggg tcctccatcc catgctggct
    12661 catagggtgt aacaagctgt tgttctctcc ttcattggcc tcttctactt gctctggttc
    12721 aactggtact aacttgaagc accatccaaa ggtcagtgga tatctgatcc ctggccctgg
    12781 tgtgtagttc tgccaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat
    12841 ctcttgtctt ttttgggacc aaattagccc ttccagcctt tcctggtgta agtagaactg
    12901 gggctttccc taggttctgt tcatcaggtg gatggctgga agctgcgctg gtccctccct
    12961 ccacaccaag gactctgatg cgcaggtgtg cttgcacctc cgacatggca gttcctccgg
    13021 ccatgtgcac gtggagggcg gcccattgtg gtagccttgg gaggtgtctt ggttatcttg
    13081 tgtcactgtg acctgtggag atccaactga ggtagtcatc acccctcggg ggacacattc
    13141 caagacccag gtggaggcct gaaatgcgat agtactgaac cctgtatata ctgttttccc
    13201 ctctgggaac acacctctga tcaaatttaa cttacaagtt aggcacagta gagactaaaa
    13261 aaattgtgaa tcaaatggaa tgattgtaag ggtgcactgt gataaacccc ctgtgaggct
    13321 gacgactgtc agggtgcaat gtgataaacc ccctgtgagg ctgacgactg tcagggtgca
    13381 ctgtgataaa ccccctgtaa agctggtctc tttctctcgc agtcttaccg ctctgtgcgc
    13441 acctagtttc agaccttggc tgaacgcaag taactgaaac tagggaaagc aaagccgagt
    13501 gggggcagcc gctagaagga aggggcagcc tcagggcaca ctgtggggct tggcagttag
    13561 ggaccctgca gagtccacat gccagttggt tggcagcaca gcagagcgcc cggaaggggc
    13621 acatgccttt gacaggagtt agatgtggtg gagagaggcg cttaaaatta ttgaggacaa
    13681 gatgggttat ttggtattta atgctaacag ctgtatctcc attttgatgt gaaggagtga
    13741 gctagatttt atttattttt tattatttta ttttttacct ttaggctctg ctggactggc
    13801 ctagattttg tatacacaca tttcaggtga aggtctaaaa aaaactggag catataaaga
    13861 aaaatagaat atatgtgtaa ttctggaata aggaaggact gtgtttccaa aatagaaaat
    13921 aaataaataa ataaaggaaa agattggaat atctgattga tttatttatt ttatttttga
    13981 gacagagtct tactctgtcg cccaggctgg agtgcagtgc tgcgatctca gcttactgca
    14041 atctctgcct cccctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    14101 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
    14161 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    14221 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    14281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    14341 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
    14401 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
    14461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    14521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    14581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    14641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    14701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    14761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    14821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    14881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    14941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    15001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    15061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    15121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    15181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    15241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    15301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    15361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    15421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    15481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    15541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    15601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    15661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    15721 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    15781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    15841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    15901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    15961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    16021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    16081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    16141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    16201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    16261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    16321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    16381 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcga ctggtgtcag aagcgggcag
    16441 tctttgaacc accattgcaa aattataact cagacagtga aagagatctg acctaaccaa
    16501 ttccatcgtg cttctaacct ccaagctctc cttgttcatt cctgggtgta ggccgaacta
    16561 agtttaggag gaacttagtt tatagtttga aacaaagaca gtaacagccc tttcccgaaa
    16621 caaaccccct ttttgcctgg ggactagact gcctttgtag gactaacaaa ttagccaaaa
    16681 gattagaaat tatggtttag ggccaggtgc agtgactggc cccggttgtc ccagcacttt
    16741 gggaggctga ggctggatca cttgagccca ggagttcgag accagccagg gccacacggc
    16801 aaaacgctgc ctctaccaaa aatacaaaaa ttaactgggg cgtcgcttgt agtctcagct
    16861 accaggcagg ctgaggtggg aggatcgctt gagcttacga cgttgaggct gcagtgagct
    16921 gtgattgcac cactgcactc cagcctaggt gacagagtga gaccctgtct caaaaataaa
    16981 aaaaaaaaaa caaaaaagaa attatggttt aggagtcatg cagctacagg ctacaagatt
    17041 ctgaccttcc ccaaattgtt cctggggtaa catcactatt gtaaaaccta agatcaatca
    17101 gtgcttgaga tattttgcag accctgcact ccatggatca gctggcaccc ccggattgat
    17161 aaactggctc atccgatctt gtggccccca cccaggaact gactcggcgc aaaaggacac
    17221 cttcgactcc ctacgctttc atctccaact cactggcccc ctacacacta agttatcctt
    17281 aaaaactctg atccccgaat gcttggggag actgatttga gtcataatag aactccgttc
    17341 tcccgcacag ccggctctgt gtgacttact ctttctctat cctaattccc gtcttggtga
    17401 atcggctctg cctgggcagc aggcggggtg gacctgttgg gtgtttatgt cttgggtgtt
    17461 tatgtcttgg ggactgagcc ctcaccctgt ggtctgacct ctctccaggc aggcggtgtt
    17521 ggaactgaac cgaaggacac ccgccgagtc tgcggcagaa ctgactgctc atgtgctggt
    17581 gaaaacctcc ctctcccacc acatttgatc acagaagtct tcctctgtgc tgatgattct
    17641 tgtggtatca gagtagagga aaaacagttt cagttttcca gaaatacctg tgaataaaat
    17701 acacataaaa taaaatctac taaaaataca cagagaaggg gtttctccat gttggtcagg
    17761 ctggtctgga actcccaaac tcaggtgatc cacctgcctc ggcctcccaa agggccgggc
    17821 gcaggtgtga gcc
//
