LOCUS       sequence-352470-        9260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9260)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305
FEATURES             Location/Qualifiers
     source          1..9260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..225
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        37..233
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      84..219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             265..867
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(265..284)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     761..781
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    910..1027
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1085..1585
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1504..1522
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    1606..1730
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1755..1760,1761..1778,1779..1785)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1839..2498
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1905..1924)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2840..3145
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3024..3043
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3186..3310)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3362..3950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3415..3435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3833..3844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4022..4255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4333..4454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4370..4389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4424..4443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4494..4629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4649..4668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4650..4668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4678..4695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4678..4694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4687..4709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4836..5291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4923..4942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5133..5154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5317..5421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5422..5490,5491..6282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5640..5659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6453..7041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6942..6961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7195..7212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7329..7350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7365..7370,7371..7388,7389..7395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7403..7419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7408..7430
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7427..7443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7427..7443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7462..7482
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7464..7482
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7510..7736
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7737..7917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7964..8089
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8582..8815
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9000..9044
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag tcagcaacca
       61 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      121 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      181 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt
      241 ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
      301 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
      361 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
      421 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
      481 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
      541 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
      601 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
      661 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
      721 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
      781 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
      841 atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa tggcagtatt
      901 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      961 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1021 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgaggcctg
     1081 caggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     1141 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     1201 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     1261 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     1321 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     1381 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     1441 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     1501 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     1561 ccgaatcacc gacctctctc cccaggggct agcatcgatt gatcaacaag tttgtacaaa
     1621 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1681 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     1741 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     1801 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1861 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1921 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1981 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     2041 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     2101 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     2161 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     2221 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     2281 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     2341 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     2401 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     2461 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     2521 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     2581 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     2641 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     2701 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     2761 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     2821 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     2881 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     2941 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
     3001 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     3061 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     3121 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     3181 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     3241 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     3301 acaaagtggt taccggttag taatgaacta gttaagtcga caatcaacgc gttaagtcga
     3361 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3841 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3901 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     3961 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4021 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     4261 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     4321 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4381 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4441 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     4501 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4561 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4621 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     4681 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4741 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4801 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4981 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5041 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5101 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5161 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5221 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5281 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5341 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7141 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7201 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7261 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7321 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7381 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7441 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     7501 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     7561 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     7621 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     7681 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     7741 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7801 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7861 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7921 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7981 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8041 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8101 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8161 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8221 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8281 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8341 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8401 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     8461 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     8521 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     8581 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     8641 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8701 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8761 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8821 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8881 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8941 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     9001 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     9061 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     9121 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     9181 tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc
     9241 agcaaccagg tgtggaaagt
//
