LOCUS       sequence-35248-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET MBP mCerulean LIC cloning vector (MBP-mCer...
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..200
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             216..233
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatatagc atgggttctt ctggaggcct gaacgatatt tttgaagcgc
      181 agaaaattga atggcatgaa tccatgggtt cttctcacca tcaccatcac cacggttctt
      241 ctatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
      301 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
      361 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
      421 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
      481 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
      541 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
      601 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      661 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      721 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacg
//
