LOCUS       sequence-35250-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET mOrange LIC cloning vector (u-mOrange)
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             120..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     593..612
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagtgccg
       61 gctccggaga gctctttaat taagcggccg ccctgcagga ctcgagttct agaaataatt
      121 ttgtttaact ttaagaagga gatatagata tcctctcatc ctccaacggc agcagcggat
      181 ccgtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      241 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      301 gcccctacga gggctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
      361 tcgcctggga catcctgtcc cctcagttca cctacggctc caaggcctac gtgaagcacc
      421 ccgccgacat ccccgactac ttcaagctgt ccttccccga gggcttcaag tgggagcgcg
      481 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctccaggacg
      541 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      601 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      661 ccctgaaggg cgagatcaag atga
//
