LOCUS       sequence-352510-       10717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Vaccari Cardoso B, Barrera I, Mosienko V, Gourine AV, Kasparov S, 
            Teschemacher AG
  TITLE     Expression of Microbial Enzymes in Mammalian Astrocytes to Modulate 
            Lactate Release.
  JOURNAL   Brain Sci. 2021 Aug 10;11(8). pii: brainsci11081056. doi: 
            10.3390/brainsci11081056.
  PUBMED    34439675
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain 
            Sci."; date: "2021-08-10"; volume: "11(8). pii: brainsci11081056. 
            doi"; pages: " 10.3390/brainsci11081056"
COMMENT     Sequence Label: pTYF-EF1a-DLDH-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..10717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1387..1396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1393..1395,1396..1398,1399..2112)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1393..1395,1396..1398,1399..2109)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1438..1459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1699..1718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2046..2067
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2323..2547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2680..2699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(3721..3738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3892..4480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3972..3991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4651..5442,5443..5511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5274..5293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5512..5616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5684..5702
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5740..5762)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5862..5881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7143..7163
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7206..7386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8768..9001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9263..9380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9535..10713
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9766..10704
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10661..10681
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ctagcatgac caagatcttc gcctacgcca tcagagagga cgagaagccc ttcctgaaag
       61 agtgggagga cgcccacaag gacgtggaag tggagtacac cgacaagctg ctgaccccag
      121 agacagccgc cctggccaaa ggcgctgatg gcgtggtggt gtaccagcag ctggactaca
      181 cagccgagac actgcaggcc ctggccgaca acggcatcac caagatgagc ctgcggaacg
      241 tgggcgtgga caacatcgac atggccaagg ccaaagagct gggcttccag atcaccaacg
      301 tgcccgtgta cagccccaac gccattgccg aacacgccgc catccaggcc gccagaatcc
      361 tgcggcaggc caaagccatg gacgagaagg tggcccggca cgacctgaga tgggccccca
      421 ccatcggcag agaagtgcgg gaccaggtcg tgggcgtcgt gggaacaggc cacatcggcc
      481 aggtgttcat gcagatcatg gaaggcttcg gcgccaaagt gatcgcctac gacatcttcc
      541 ggaaccccga gctggaaaag aaaggctact acgtggacag cctggacgac ctgtacaagc
      601 aggccgacgt gatcagcctg cacgtgcccg atgtgcccgc caacgtgcac atgatcaacg
      661 acaagtctat cgccaagatg aagcaggacg tggtgatcgt gaacgtgtcc agaggccccc
      721 tggtggacac cgacgccgtg atcagaggcc tggacagcgg caaggtgttc ggctacgcca
      781 tggacgtgta cgagggcgaa gtgggcgtgt tcaacgagga ctgggagggc aaagagttcc
      841 ccgacgccag actggccgac ctgatcgcca gacccaacgt gctggtgaca ccccacaccg
      901 ccttctacac cacccacgcc gtgcggaaca tggtgatcaa ggccttcgac aacaacctgg
      961 aactgatcga gggaaaagag gccgagacac ccgtgaaagt gggctgagtc gacggtacca
     1021 gatgggggtc ctgggcccca gggtgtgcag ccactgactt ggggactgct ggtggggtag
     1081 ggatgaggga gggaggggca ttgtgatgta cagggctgct ctgtgagatc aagggtctct
     1141 taagggtggg agctggggca gggactacga gagcagccag atgggctgaa agtggaactc
     1201 aaggggtttc tggcacctac ctacctgctt cccgctgggg ggtggggagt tggcccagag
     1261 tcttaagatt ggggcagggt ggagaggtgg gctcttcctg cttcccactc atcttatagc
     1321 tttctttccc cagatccgaa ttcgagatcc aaaccaaggg taccgcgggc ccgggatcca
     1381 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1441 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1501 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1561 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1621 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1681 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1741 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1801 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1861 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1921 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1981 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2041 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2101 ctgtacaagt aaagcggccg catcgattag ggataacagg gtaacggtac ctttaagacc
     2161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2221 agggctaatt cactcccagg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2281 ggctaactag ggaacccact gcttaagcct cataaagctc gactgtgcct tctagttgcc
     2341 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2401 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2461 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2521 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     2581 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2641 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2701 cctttctcgc cacgttcgcc ggctgtgcgg gagaacggag ttctattatg actcaaatca
     2761 gtctccccaa gcattcgggg atcagagttt ttaaggataa cttagtgtgt agggggccag
     2821 tgagttggag atgaaagcgt agggagtcga aggtgtcctt ttgcgccgag tcagttcctg
     2881 ggtgggggcc acaagatcgg atgagccagt ttatcaatcc gggggtgcca gctgatccat
     2941 ggagtgcagg gtctgcaaaa tatctcaagc actgattgat cttaggtttt acaatagtga
     3001 tgttacccca ggaacaattt ggggaaggtc agaatcttgt agcctgtagc tgcatgactc
     3061 ctaaaccata atttcttttt tgtttttttt tttttatttt tgagacaggg tctcactctg
     3121 tcacctaggc tggagtgcag tggtgcaatc acagctcact gcagcctcaa cgtcgtaagc
     3181 tcaagcgatc ctcccacctc agcctgcctg gtagctgaga ctacaagcga cgccccagtt
     3241 aatttttgta tttttggtag aggcagcgtt ttgccgtgtg gccctggctg gtctcgaact
     3301 cctgggctca agtgatccag cctcagcctc ccaaagtgct gggacaaccg gggccagtca
     3361 ctgcacctgg ccctaaacca taatttctaa tcttttggct aatttgttag tcctacaaag
     3421 gcagtctagt ccccaggcaa aaagggggtt tgtttcggga aagggctgtt actgtctttg
     3481 tttcaaacta taaactaagt tcctcctaaa cttagttcgg cctacaccca ggaatgaaca
     3541 aggagagctt ggaggttaga agcacgatgg aattggttag gtcagatctc tttcactgtc
     3601 tgagttataa ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc agtcgctgca
     3661 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3721 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3781 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3841 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4321 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4501 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4561 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4621 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4681 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4741 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4801 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4861 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4921 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4981 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5041 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5101 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5161 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5221 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5281 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5341 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5401 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5461 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5521 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5581 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5641 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5701 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5761 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5821 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     5881 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5941 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6001 ctcttcgcta ttacgccagg ggaggcagag attgcagtaa gctgagatcg cagcactgca
     6061 ctccagcctg ggcgacagag taagactctg tctcaaaaat aaaataaata aatcaatcag
     6121 atattccaat cttttccttt atttatttat ttattttcta ttttggaaac acagtccttc
     6181 cttattccag aattacacat atattctatt tttctttata tgctccagtt ttttttagac
     6241 cttcacctga aatgtgtgta tacaaaatct aggccagtcc agcagagcct aaaggtaaaa
     6301 aataaaataa taaaaaataa ataaaatcta gctcactcct tcacatcaaa atggagatac
     6361 agctgttagc attaaatacc aaataaccca tcttgtcctc aataatttta agcgcctctc
     6421 tccaccacat ctaactcctg tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc
     6481 caaccaactg gcatgtggac tctgcagggt ccctaactgc caagccccac agtgtgccct
     6541 gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt
     6601 tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga
     6661 aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag
     6721 ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca
     6781 cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta
     6841 agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta
     6901 ctatcgcatt tcaggcccga gagctgcatc cggagtatct agatggagtt ccgcgttaca
     6961 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7021 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggag
     7081 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
     7141 gacgcaaatg ggcggtaggc gtgtactcta gaaggtctat ataagcagag ctcgtttagt
     7201 gaaccgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7261 acccactgct taagcctcaa taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc
     7321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7381 ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta aagccagagg agatctctcg
     7441 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactgcagag
     7501 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg
     7561 tattaagcgg gggagaatta gataaatggg aaaaaattcg gttaaggcca gggggaaaga
     7621 aacaatataa actaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7681 atcctggcct tttagagaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7741 cccttcagac aggatcagaa gaacttagat cattatataa tacaatagca gtcctctatt
     7801 gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc cttagataag atagaggaag
     7861 agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc tgacacagga aacaacagcc
     7921 aggtcagcca aaattaccct atagtgcaga acctccaggg gcaaatggta catcaggcca
     7981 tatcacctag aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag
     8041 aagtaatacc catgttttca gcattatcag aaggagccac cccacaagat ttaaatacca
     8101 tgctaaacac agtgggggga catcaagcag ccatgcaaat gttaaaagag accatcaatg
     8161 aggaagctgc agaatgggat agattgcatc cagtgcatgc agggcctatt gcaccaggcc
     8221 agatgagaga accaagggga agtgacatag caggaactac tagcaaatta agagaacaat
     8281 ttggaaataa taaaacaata atctttaagc aatcctcagg aggggaccca gaaattgtaa
     8341 cgcacagttt taattgtgga ggggaatttt tctactgtaa ttcaacacaa ctgtttaata
     8401 gtacttggtt taatagtact tggagtactg aagggtcaaa taacactgaa ggaagtgaca
     8461 caatcacact cccatgcaga ataaaacaat ttataaacat gtggcaggaa gtaggaaaag
     8521 caatgtatgc ccctcccatc agtggacaaa ttagatgttc atcaaatatt actgggctgc
     8581 tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag
     8641 gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8701 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8761 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8821 cgtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga
     8881 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8941 tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9001 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9061 gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca
     9121 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9181 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcttgcggc cggaattcac
     9241 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9301 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9361 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttgggcgg
     9421 cctagggata acagggtaat gatcgcgtaa gctttgcaaa gatggataaa gttttaaaca
     9481 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     9541 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     9601 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     9661 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     9721 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     9781 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     9841 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     9901 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     9961 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
    10021 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
    10081 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
    10141 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
    10201 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
    10261 gcgccgccgt gtatcgcccc gccctaggcg gcaaggctgg cccggtcggc accagttgcg
    10321 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
    10381 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
    10441 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
    10501 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
    10561 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
    10621 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
    10681 caaagttttt ttcttccatt tcaggtgtcg tgaggtg
//
