LOCUS       sequence-35253-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET mCerulean LIC cloning vector (u-mCerulean)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(265..286)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(526..545)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatagata tcctctcatc ctccaacggc agcagcggat ccgtgagcaa gggcgaggag
      241 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg
      301 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      361 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
      421 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      481 gccatgcccg aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac
      541 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      601 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
      661 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag
      721 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      781 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
      841 ctg
//
