LOCUS       sequence-352553-       10203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 CC1D
FEATURES             Location/Qualifiers
     source          1..10203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..157
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        209..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     713..737
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      796..805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(802..804,805..807,808..1518)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(802..804,805..807,808..1518)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(847..868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1108..1127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1455..1476
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1534..1558
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3434..3458)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3477..3964
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3512..3535)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3897..3916
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3988..4386
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4428..4549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4465..4484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    4575..5163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4628..4648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5046..5057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5238..5471
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5590..5608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5676..5780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5781..5849,5850..6641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5999..6018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6812..7400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7301..7320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7554..7571
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7811..7832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7847..7852,7853..7870,7871..7877)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7885..7901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7890..7912
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7909..7925
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7909..7925
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8186..8819
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8866..8991
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9488..9721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9906..9950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
       61 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      121 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      181 agatccagtt tggactagtg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
      721 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      781 accggcggcc gatctgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      841 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      961 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1081 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1201 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1261 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1381 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1501 atggacgagc tgtacaaggc tcgaggttat caaacaagtt tgtacaaaaa agttggcatg
     1561 acatcctttc aagaagtccc attgcagact tccaactttg cccatgtcat ctttcaaaat
     1621 gtggccaaga gttaccttcc taatgcacac ctggaatgtc attacacctt aactccatat
     1681 attcatccac atccaaaaga ttgggttggt atattcaagg ttggatggag tactgctcgt
     1741 gattattaca cgtttttatg gtcccctatg cctgaacatt atgtggaagg atcaacagtc
     1801 aattgtgtac tagcattcca aggatattac cttccaaatg atgatggaga attttatcag
     1861 ttctgttacg ttacccataa gggtgaaatt cgtggagcaa gtacaccttt ccagtttcga
     1921 gcttcttctc cagttgaaga gctgcttact atggaagatg aaggaaattc tgacatgtta
     1981 gtggtggatg ggaacaaaga aagcgtgatt actcatttca aagaagagat tggcaggctg
     2041 cagttatgtt tggctgaaaa ggaaaatctg caaagaactt tcctgcttac aacctcaagt
     2101 aaagaagata cttgtttttt aaaggagcaa cttcgtaaag cagaggaaca ggttcaggca
     2161 actcggcaag aagttgtctt tctggctaaa gaactcagtg atgctgtcaa cgtacgagac
     2221 agaacgatgg cagacctgca tactgcacgc ttggaaaacg agaaagtgaa aaagcagtta
     2281 gctgatgcag tggcagaact taaactaaat gctatgaaaa aagatcagga caagactgat
     2341 acactggaac acgaactaag aagagaagtt gaagatctga aactccgtct tcagatggct
     2401 gcagaccatt ataaagaaaa atttaaggaa tgccaaaggc tccaaaaaca aataaacaaa
     2461 ctttcagatc aatcagctaa taataataat gtcttcacaa agaaaacggg gaatcagcag
     2521 aaagtgaatg atgcttcagt aaacacagac ccagccactt ctgcctctac tgtagatgta
     2581 aagccatcac cttctgcagc agaggcagat tttgacatag taacaaaggg gcaagtctgt
     2641 gaaatgacca aagaaattgc tgacaaaaca gaaaagtata ataaatgtaa acaactcttg
     2701 caggatgaga aagcaaaatg caataaatat gctgatgaac ttgcaaaaat ggagctgaaa
     2761 tggaaagaac aagtgaaaat tgctgaaaat gtaaaacttg aactagctga agtacaggac
     2821 aattataaag aacttaaaag gagtctagaa aatccagcag aaaggaaaat ggaaggtcag
     2881 aattcccaga gtcctcaatg tttcaaaaca tgctcagagc aaaatggtta tgttctcaca
     2941 ttgtcaaatg cacaaccagt tctgcaatat ggtaatcctt atgcatctca ggaaacaaga
     3001 gatggagcag atggtgcttt ttacccagat gaaatacaaa ggccacctgt cagagtcccc
     3061 tcttggggac tggaagacaa tgttgtctgc agccagcctg ctcgaaactt tagtcggcct
     3121 gatggcttag aggactctga ggatagcaaa gaagatgaga atgtgcctac tgctcctgat
     3181 cctccaagtc aacatttacg tgggcatggg acaggctttt gctttgattc cagctttgat
     3241 gttcacaaga agtgtcccct ctgtgagtta atgtttcctc ctaactatga tcagagcaaa
     3301 tttgaagaac atgttgaaag tcactggaag gtgtgcccga tgtgcagcga gcagttccct
     3361 cctgactatg accagcaggt gtttgaaagg catgtgcaga cccattttga tcagaatgtt
     3421 ctaaattttg actacccaac tttcttgtac aaagtggttc gataacgaat tctaccgggt
     3481 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     3541 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3601 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     3661 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     3721 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     3781 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     3841 gtccgggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     3901 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     3961 ttcgacctgc agcccattaa ttaaaccatg gccaagcctt tgtctcaaga agaatccacc
     4021 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     4081 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     4141 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     4201 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     4261 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     4321 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     4381 ggctaagcac ttcgtggccg aggagcagga ctgacactcg acctcgaaac ttgtttattg
     4441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4501 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctatg
     4561 catcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4621 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4681 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4741 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4801 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4861 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4921 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4981 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5041 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5101 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5161 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5221 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5281 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5341 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5401 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5461 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     5521 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     5581 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5641 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5701 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5761 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5821 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5881 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5941 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6001 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     6061 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6121 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6181 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6241 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6301 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6361 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6421 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6481 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6541 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6601 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6661 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6721 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7441 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7501 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7561 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7621 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     7681 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7741 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7801 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7861 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7921 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7981 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8041 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8101 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8161 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     8221 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8281 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8341 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8401 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8461 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8521 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8581 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     8641 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8701 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8761 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8821 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8881 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8941 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9001 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9061 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     9361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    10081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    10141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
    10201 gac
//
