LOCUS       sequence-352555-       10683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 
            632-639D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 632-639D
FEATURES             Location/Qualifiers
     source          1..10683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        293..596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        597..800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     751..771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     797..821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      880..889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(886..888,889..891,892..1602)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(886..888,889..891,892..1602)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(931..952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1192..1211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1539..1560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1618..1642
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3986..4010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4029..4528
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4064..4087)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4461..4480
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4552..4950
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4992..5113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5029..5048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5139..5727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5192..5212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5610..5621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5802..6035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6154..6172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6240..6344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6345..6413,6414..7205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6563..6582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7376..7964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7865..7884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8118..8135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8375..8396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8411..8416,8417..8434,8435..8441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8449..8465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8454..8476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8473..8489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8473..8489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8750..9383
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9430..9555
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10052..10285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10470..10514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca
      121 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      241 tcgggtttat tacagggaca gcagagatcc agtttggact agtggagttc cgcgttacat
      301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      781 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      841 gctgttttga cctccataga agacaccggc ggccgatctg ccaccatggt gagcaagggc
      901 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      961 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1021 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1081 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1141 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1201 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1261 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1321 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1381 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1441 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1501 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1561 accgccgccg ggatcactct cggcatggac gagctgtaca aggctcgagg ttatcaaaca
     1621 agtttgtaca aaaaagttgg catgacatcc tttcaagaag tcccattgca gacttccaac
     1681 tttgcccatg tcatctttca aaatgtggcc aagagttacc ttcctaatgc acacctggaa
     1741 tgtcattaca ccttaactcc atatattcat ccacatccaa aagattgggt tggtatattc
     1801 aaggttggat ggagtactgc tcgtgattat tacacgtttt tatggtcccc tatgcctgaa
     1861 cattatgtgg aaggatcaac agtcaattgt gtactagcat tccaaggata ttaccttcca
     1921 aatgatgatg gagaatttta tcagttctgt tacgttaccc ataagggtga aattcgtgga
     1981 gcaagtacac ctttccagtt tcgagcttct tctccagttg aagagctgct tactatggaa
     2041 gatgaaggaa attctgacat gttagtggtg accacaaaag caggccttct tgagttgaaa
     2101 attgagaaaa ccatgaaaga aaaagaagaa ctgttaaagt taattgccgt tctggaaaaa
     2161 gaaacagcac aacttcgaga acaagttggg agaatggaaa gagaacttaa ccatgagaaa
     2221 gaaagatgtg accaactgca agcagaacaa aagggtctta ctgaagtaac acaaagctta
     2281 aaaatggaaa atgaagagtt taagaagagg ttcagtgatg ctacatccaa agcccatcag
     2341 cttgaggaag atattgtgtc agtaacacat aaagcaattg aaaaagaaac cgaattagac
     2401 agtttaaagg acaaactcaa gaaggcacaa catgaaagag aacaacttga atgtcagttg
     2461 aagacagaga aggatgaaaa ggaactttat aaggtacatt tgaagaatac agaaatagaa
     2521 aataccaagc ttatgtcaga ggtccagact ttaaaaaatt tagatgggaa caaagaaagc
     2581 gtgattactc atttcaaaga agagattggc aggctgcagt tatgtttggc tgaaaaggaa
     2641 aatctgcaaa gaactttcct gcttacaacc tcaagtaaag aagatacttg ttttttaaag
     2701 gagcaacttc gtaaagcaga ggaacaggtt caggcaactc ggcaagaagt tgtctttctg
     2761 gctaaagaac tcagtgatgc tgtcaacgta cgagacagaa cgatggcaga cctgcatact
     2821 gcacgcttgg aaaacgagaa agtgaaaaag cagttagctg atgcagtggc agaacttaaa
     2881 ctaaatgcta tgaaaaaaga tcaggacaag actgatacac tggaacacga actaagaaga
     2941 gaagttgaag atctgaaact ccgtcttcag atggctgcag accattataa agaaaaattt
     3001 aaggaatgcc aaaggctcca aaaacaaata aacaaacttt cagatcaatc agctaataat
     3061 aataatgtct tcacaaagaa aacggggaat cagcagaaag tgaatgatgc ttcagtaaac
     3121 acagacccag ccacttctgc ctctactgta gatgtaaagc catcaccttc tgcagcagag
     3181 gcagattttg acatagtaac aaaggggcaa gtctgtgaaa tgaccaaaga aattgctgac
     3241 aaaacagaaa agtataataa atgtaaacaa ctcttgcagg atgagaaagc aaaatgcaat
     3301 aaatatgctg atgaacttgc aaaaatggag ctgaaatgga aagaacaagt gaaaattgct
     3361 gaaaatgtaa aacttgaact agctgaagta caggacaatt ataaagaact taaaaggagt
     3421 ctagaaaatc cagcagaaag gaaaatggaa ggtcagaatt cccagagtcc tcaatgtttc
     3481 aaaacatgct cagagcaaaa tggttatgtt ctcacattgt caaatgcaca accagcatct
     3541 caggaaacaa gagatggagc agatggtgct ttttacccag atgaaataca aaggccacct
     3601 gtcagagtcc cctcttgggg actggaagac aatgttgtct gcagccagcc tgctcgaaac
     3661 tttagtcggc ctgatggctt agaggactct gaggatagca aagaagatga gaatgtgcct
     3721 actgctcctg atcctccaag tcaacattta cgtgggcatg ggacaggctt ttgctttgat
     3781 tccagctttg atgttcacaa gaagtgtccc ctctgtgagt taatgtttcc tcctaactat
     3841 gatcagagca aatttgaaga acatgttgaa agtcactgga aggtgtgccc gatgtgcagc
     3901 gagcagttcc ctcctgacta tgaccagcag gtgtttgaaa ggcatgtgca gacccatttt
     3961 gatcagaatg ttctaaattt tgactaccca actttcttgt acaaagtggt tcgataacga
     4021 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     4081 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     4141 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     4201 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     4261 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     4321 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     4381 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     4441 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     4501 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca ttaattaaac catggccaag
     4561 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     4621 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     4681 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     4741 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     4801 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     4861 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     4921 ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca
     4981 ctcgacctcg aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5101 tcaatgtatc ttatcatgtc tatgcatcga tagatcctaa tcaacctctg gattacaaaa
     5161 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5221 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5281 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5341 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5401 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5461 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5521 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5581 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5641 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5701 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     5761 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5821 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6001 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6061 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     6121 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     6181 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6241 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6301 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6361 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6421 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6481 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6541 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     6601 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6661 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6721 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6781 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6841 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6901 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6961 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7021 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7081 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7141 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7201 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7261 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7321 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7381 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7441 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7501 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7561 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7621 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7681 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7741 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7801 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7861 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7921 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7981 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8041 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8101 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8161 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
     8221 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8281 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
     8341 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8401 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8461 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
     8521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8581 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     8641 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     8701 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     8761 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8821 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8881 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8941 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     9001 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     9061 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     9121 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     9181 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9241 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9301 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9361 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9421 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9481 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9541 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9601 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9661 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9721 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9781 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9841 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     9901 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9961 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10021 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    10081 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    10141 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10201 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10261 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10321 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10381 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10441 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10501 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10561 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10621 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10681 ggg
//
