LOCUS       sequence-352557-       10707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 N637A
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..204
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        256..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     714..734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     760..784
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      843..852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(849..851,852..854,855..1565)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(849..851,852..854,855..1565)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(894..915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1155..1174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1502..1523
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1581..1605
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3973..3997)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4016..4515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4051..4074)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4448..4467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4539..4937
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4979..5100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5016..5035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5126..5714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5179..5199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5597..5608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5789..6022
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6141..6159)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6227..6331
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6332..6400,6401..7192)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6550..6569)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7363..7951
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7852..7871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8105..8122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8362..8383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8398..8403,8404..8421,8422..8428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8441..8463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8460..8476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8460..8476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8737..9370
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9417..9542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10039..10272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10457..10501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
       61 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      121 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      181 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      241 actagtggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc ggcggccgat
      841 ctgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1021 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1261 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1321 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1501 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1561 acaaggctcg aggttatcaa acaagtttgt acaaaaaagt tggcatgaca tcctttcaag
     1621 aagtcccatt gcagacttcc aactttgccc atgtcatctt tcaaaatgtg gccaagagtt
     1681 accttcctaa tgcacacctg gaatgtcatt acaccttaac tccatatatt catccacatc
     1741 caaaagattg ggttggtata ttcaaggttg gatggagtac tgctcgtgat tattacacgt
     1801 ttttatggtc ccctatgcct gaacattatg tggaaggatc aacagtcaat tgtgtactag
     1861 cattccaagg atattacctt ccaaatgatg atggagaatt ttatcagttc tgttacgtta
     1921 cccataaggg tgaaattcgt ggagcaagta cacctttcca gtttcgagct tcttctccag
     1981 ttgaagagct gcttactatg gaagatgaag gaaattctga catgttagtg gtgaccacaa
     2041 aagcaggcct tcttgagttg aaaattgaga aaaccatgaa agaaaaagaa gaactgttaa
     2101 agttaattgc cgttctggaa aaagaaacag cacaacttcg agaacaagtt gggagaatgg
     2161 aaagagaact taaccatgag aaagaaagat gtgaccaact gcaagcagaa caaaagggtc
     2221 ttactgaagt aacacaaagc ttaaaaatgg aaaatgaaga gtttaagaag aggttcagtg
     2281 atgctacatc caaagcccat cagcttgagg aagatattgt gtcagtaaca cataaagcaa
     2341 ttgaaaaaga aaccgaatta gacagtttaa aggacaaact caagaaggca caacatgaaa
     2401 gagaacaact tgaatgtcag ttgaagacag agaaggatga aaaggaactt tataaggtac
     2461 atttgaagaa tacagaaata gaaaatacca agcttatgtc agaggtccag actttaaaaa
     2521 atttagatgg gaacaaagaa agcgtgatta ctcatttcaa agaagagatt ggcaggctgc
     2581 agttatgttt ggctgaaaag gaaaatctgc aaagaacttt cctgcttaca acctcaagta
     2641 aagaagatac ttgtttttta aaggagcaac ttcgtaaagc agaggaacag gttcaggcaa
     2701 ctcggcaaga agttgtcttt ctggctaaag aactcagtga tgctgtcaac gtacgagaca
     2761 gaacgatggc agacctgcat actgcacgct tggaaaacga gaaagtgaaa aagcagttag
     2821 ctgatgcagt ggcagaactt aaactaaatg ctatgaaaaa agatcaggac aagactgata
     2881 cactggaaca cgaactaaga agagaagttg aagatctgaa actccgtctt cagatggctg
     2941 cagaccatta taaagaaaaa tttaaggaat gccaaaggct ccaaaaacaa ataaacaaac
     3001 tttcagatca atcagctaat aataataatg tcttcacaaa gaaaacgggg aatcagcaga
     3061 aagtgaatga tgcttcagta aacacagacc cagccacttc tgcctctact gtagatgtaa
     3121 agccatcacc ttctgcagca gaggcagatt ttgacatagt aacaaagggg caagtctgtg
     3181 aaatgaccaa agaaattgct gacaaaacag aaaagtataa taaatgtaaa caactcttgc
     3241 aggatgagaa agcaaaatgc aataaatatg ctgatgaact tgcaaaaatg gagctgaaat
     3301 ggaaagaaca agtgaaaatt gctgaaaatg taaaacttga actagctgaa gtacaggaca
     3361 attataaaga acttaaaagg agtctagaaa atccagcaga aaggaaaatg gaaggtcaga
     3421 attcccagag tcctcaatgt ttcaaaacat gctcagagca aaatggttat gttctcacat
     3481 tgtcaaatgc acaaccagtt ctgcaatatg gtgcacctta tgcatctcag gaaacaagag
     3541 atggagcaga tggtgctttt tacccagatg aaatacaaag gccacctgtc agagtcccct
     3601 cttggggact ggaagacaat gttgtctgca gccagcctgc tcgaaacttt agtcggcctg
     3661 atggcttaga ggactctgag gatagcaaag aagatgagaa tgtgcctact gctcctgatc
     3721 ctccaagtca acatttacgt gggcatggga caggcttttg ctttgattcc agctttgatg
     3781 ttcacaagaa gtgtcccctc tgtgagttaa tgtttcctcc taactatgat cagagcaaat
     3841 ttgaagaaca tgttgaaagt cactggaagg tgtgcccgat gtgcagcgag cagttccctc
     3901 ctgactatga ccagcaggtg tttgaaaggc atgtgcagac ccattttgat cagaatgttc
     3961 taaattttga ctacccaact ttcttgtaca aagtggttcg ataacgaatt ctaccgggta
     4021 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     4081 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     4141 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4201 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4261 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     4321 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     4381 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     4441 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     4501 tctccgggcc tttcgacctg cagcccatta attaaaccat ggccaagcct ttgtctcaag
     4561 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     4621 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     4681 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     4741 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     4801 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     4861 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     4921 atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacactc gacctcgaaa
     4981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5101 tcatgtctat gcatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt
     5161 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5221 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5281 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5341 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5401 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5461 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5521 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5581 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5641 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5701 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt
     5761 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5821 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5881 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5941 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6001 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6061 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga
     6121 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6181 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6541 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     6601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6841 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7501 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8101 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8161 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat
     8221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8281 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc
     8341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8461 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc
     8521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     8581 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     8641 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8701 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     8761 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     8821 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     8881 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     8941 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9001 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9061 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9121 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     9181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9361 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9421 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9481 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9541 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9601 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9661 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9721 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9781 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9841 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     9901 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9961 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10021 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10081 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10141 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10201 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10261 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10321 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10381 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10441 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10501 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10561 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10621 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10681 gcccgaagga atagaagaag aaggtgg
//
