LOCUS       sequence-352559-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Kwak S, Mahmud B, Dantas G
  TITLE     A Tunable and Expandable Transactivation System in Probiotic Yeast 
            Saccharomyces boulardii.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 
            10.1021/acssynbio.1c00384. Epub 2021 Dec 23.
  PUBMED    34939781
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00384"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "508-514"
COMMENT     Sequence Label: pRS42K_PCP-VP64_A-PP7
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..72)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     172..194
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(232..250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      291..1633
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    623..670
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1660..1764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1765..1833,1834..2625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1983..2002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2796..3384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3285..3304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3538..3555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3672..3693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3708..3713,3714..3731,3732..3738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3746..3762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3751..3773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3770..3786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3770..3786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3805..3825
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3807..3825
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4594..5298
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5345..5365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5753..5902
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      5912..6211
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        6222..6490
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        6512..6587
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      6619..6638
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     complement(6656..6675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6657..6675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6685..6702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6685..6701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6694..6716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6842..7297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6929..6948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7139..7160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gene            complement(join(7388..8705,1..39))
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(7388..7585)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(7591..8400)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8314..8333
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(join(8401..8705,1..39))
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gtcaagactg tcaaggaggg tattctgggc ctccatgtcg ctgcactctc agtacaatct
       61 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      121 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      181 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      241 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagtat gatccaatat
      301 caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag
      361 aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat
      421 cacaggaggt actagactac ctttcatcct acataaatag acgcatataa gtacgcattt
      481 aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag
      541 atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag
      601 cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagaaagta
      661 taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac
      721 caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca
      781 ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca
      841 tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc
      901 tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa
      961 aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac
     1021 cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt
     1081 atgcgcaatc cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca
     1141 cccgcagctt cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa
     1201 gagaaaatag acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt
     1261 tatcaagaga ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg
     1321 ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt
     1381 tgttggtaaa atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     1441 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga
     1501 ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca
     1561 gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca
     1621 cagatgcttc gttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1681 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1741 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1801 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1861 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1921 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1981 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2041 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2101 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2161 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2221 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2281 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2341 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2401 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2461 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2521 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2581 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2641 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2701 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2761 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2821 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2881 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2941 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3001 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3061 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3121 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3181 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3241 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3301 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3361 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3421 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3481 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3541 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3601 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3661 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3721 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3781 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     3841 ctcgccggga tcgaagaaat gatggtaaat gaaataggaa atcaaggagc atgaaggcaa
     3901 aagacaaata taagggtcga acgaaaaata aagtgaaaag tgttgatatg atgtatttgg
     3961 ctttgcggcg ccgaaaaaac gagtttacgc aattgcacaa tcatgctgac tctgtggcgg
     4021 acccgcgctc ttgccggccc ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt
     4081 tttgcgcctg cattttccaa ggtttaccct gcgctaaggg gcgagattgg agaagcaata
     4141 agaatgccgg ttggggttgc gatgatgacg accacgacaa ctggtgtcat tatttaagtt
     4201 gccgaaagaa cctgagtgca tttgcaacat gagtatacta gaagaatgag ccaagacttg
     4261 cgagacgcga gtttgccggt ggtgcgaaca atagagcgac catgaccttg aaggtgagac
     4321 gcgcataacc gctagagtac tttgaagagg aaacagcaat agggttgcta ccagtataaa
     4381 tagacaggta catacaacac tggaaatggt tgtctgtttg agtacgcttt caattcattt
     4441 gggtgtgcac tttattatgt tacaatatgg aagggaactt tacacttctc ctatgcacat
     4501 atattaatta aagtccaatg ctagtagaga aggggggtaa cacccctccg cgctcttttc
     4561 cgattttttt ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc gggtgtacaa
     4621 tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg
     4681 ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca
     4741 agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata
     4801 acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc
     4861 tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt
     4921 ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga
     4981 agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag
     5041 ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac
     5101 tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa
     5161 agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc
     5221 gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata
     5281 ccaagcatac aatcaactcc aagctggccg ctctagaact agtaaaacaa tgtctcttga
     5341 gatgcccaaa aagaaaagaa aagtgggtag tatgtccaaa accatcgttc tttcggtcgg
     5401 cgaggctact cgcactctga ctgagatcca gtccaccgca gaccgtcaga tcttcgaaga
     5461 gaaggtcggg cctctggtgg gtcggctgcg cctcacggct tcgctccgtc aaaacggagc
     5521 caagaccgcg tatcgcgtca acctaaaact ggatcaggcg gacgtcgttg attccggact
     5581 tccgaaagtg cgctacactc aggtatggtc gcacgacgtg acaatcgttg cgaatagcac
     5641 cgaggcctcg cgcaaatcgt tgtacgattt gaccaagtcc ctcgtcgcga cctcgcaggt
     5701 cgaagatctt gtcgtcaacc ttgtgccgct gggccgtggc tccggacgag ccgatgctct
     5761 cgacgatttt gacctcgata tgctgggatc cgacgctctc gatgacttcg accttgacat
     5821 gctggggagt gacgctctgg acgacttcga tctcgacatg ctgggctccg acgccctgga
     5881 tgacttcgat ctggacatgc tcatcaactg agcggatctc ttatgtcttt acgatttata
     5941 gttttcatta tcaagtatgc ctatattagt atatagcatc tttagatgac agtgttcgaa
     6001 gtttcacgaa taaaagataa tattctactt tttgctccca ccgcgtttgc tagcacgagt
     6061 gaacaccatc cctcgcctgt gagttgtacc cattcctcta aactgtagac atggtagctt
     6121 cagcagtgtt cgttatgtac ggcatcctcc aacaaacagt cggttatagt ttgtcctgct
     6181 cctctgaatc gtctccctcg atatttctca tcccgggccg gtctttgaaa agataatgta
     6241 tgattatgct ttcactcata tttatacaga aacttgatgt tttctttcga gtatatacaa
     6301 ggtgattaca tgtacgtttg aagtacaact ctagattttg tagtgccctc ttgggctagc
     6361 ggtaaaggtg cgcatttttt cacaccctac aatgttctgt tcaaaagatt ttggtcaaac
     6421 gctgtagaag tgaaagttgg tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag
     6481 ataaatgatc gatctgtcga cagtgcgata cgttttagag ctagaaatag caagttaaaa
     6541 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcaac ataaggagtt
     6601 tatatggaaa cccttatgtt tttttgtttt ttatgtctcc ggggtaccca attcgcccta
     6661 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6721 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6781 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6841 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7021 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7081 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7141 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7201 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7261 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     7321 cgcatctgtg cggtatttca caccgcgttt tcgacactgg atggcggcgt tagtatcgaa
     7381 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
     7441 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
     7501 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
     7561 aaacaagaat ctttttattg tcagtactga ttagaaaaac tcatcgagca tcaaatgaaa
     7621 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     7681 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     7741 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     7801 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     7861 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7921 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     7981 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     8041 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttgcc
     8101 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     8161 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     8221 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     8281 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     8341 atcagcatcc atgttggaat ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat
     8401 ggttgtttat gttcggatgt gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt
     8461 atatgaaaga agaacctcag tggcaaatcc taacctttta tatttctcta caggggcgcg
     8521 gcgtggggac aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc
     8581 gaggagccgt aatttttgct tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat
     8641 tatacatggg gatgtatggg ctaaatgtac gggcgacagt cacatcatgc ccctgagctg
     8701 cgcac
//
