LOCUS       sequence-352560-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Kwak S, Mahmud B, Dantas G
  TITLE     A Tunable and Expandable Transactivation System in Probiotic Yeast 
            Saccharomyces boulardii.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 
            10.1021/acssynbio.1c00384. Epub 2021 Dec 23.
  PUBMED    34939781
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00384"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "508-514"
COMMENT     Sequence Label: pRS42K_PCP-VP64_B-PP7
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     168..190
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(228..246)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      287..1629
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    619..666
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1656..1760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1761..1829,1830..2621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1979..1998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2792..3380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3281..3300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3534..3551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3668..3689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3704..3709,3710..3727,3728..3734)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3742..3758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3747..3769
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3766..3782
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3766..3782
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3801..3821
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3803..3821
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4590..5294
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5341..5361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5749..5898
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      5908..6207
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        6218..6486
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        6508..6583
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      6615..6634
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     complement(6652..6671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6653..6671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6681..6698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6681..6697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6690..6712)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6838..7293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6925..6944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7135..7156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gene            complement(join(7384..8705,1..35))
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(7384..7581)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(7587..8396)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8310..8329
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(join(8397..8705,1..35))
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agactgtcaa ggagggtatt ctgggcctcc atgtcgctgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      121 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      181 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      241 cctattttta taggttaatg tcatgataat aatggtttct tagtatgatc caatatcaaa
      301 ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca
      361 gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca
      421 ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac gcatttaagc
      481 ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat
      541 aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct
      601 cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag aaagtatagg
      661 aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa
      721 aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc
      781 tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca
      841 cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg tttatctcta
      901 gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca
      961 atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt
     1021 cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc
     1081 gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg
     1141 cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga
     1201 aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc
     1261 aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag
     1321 aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt
     1381 ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
     1441 tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct
     1501 tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt
     1561 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga
     1621 tgcttcgttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3481 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3541 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3601 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3661 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3721 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3781 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcg
     3841 ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga
     3901 caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt
     3961 gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc
     4021 gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg
     4081 cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa
     4141 tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg
     4201 aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag
     4261 acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc
     4321 ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga
     4381 caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt
     4441 gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat gcacatatat
     4501 taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct cttttccgat
     4561 ttttttctaa accgtggaat atttcggata tccttttgtt gtttccgggt gtacaatatg
     4621 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
     4681 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
     4741 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
     4801 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
     4861 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
     4921 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
     4981 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     5041 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
     5101 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
     5161 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
     5221 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
     5281 gcatacaatc aactccaagc tggccgctct agaactagta aaacaatgtc tcttgagatg
     5341 cccaaaaaga aaagaaaagt gggtagtatg tccaaaacca tcgttctttc ggtcggcgag
     5401 gctactcgca ctctgactga gatccagtcc accgcagacc gtcagatctt cgaagagaag
     5461 gtcgggcctc tggtgggtcg gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag
     5521 accgcgtatc gcgtcaacct aaaactggat caggcggacg tcgttgattc cggacttccg
     5581 aaagtgcgct acactcaggt atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag
     5641 gcctcgcgca aatcgttgta cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa
     5701 gatcttgtcg tcaaccttgt gccgctgggc cgtggctccg gacgagccga tgctctcgac
     5761 gattttgacc tcgatatgct gggatccgac gctctcgatg acttcgacct tgacatgctg
     5821 gggagtgacg ctctggacga cttcgatctc gacatgctgg gctccgacgc cctggatgac
     5881 ttcgatctgg acatgctcat caactgagcg gatctcttat gtctttacga tttatagttt
     5941 tcattatcaa gtatgcctat attagtatat agcatcttta gatgacagtg ttcgaagttt
     6001 cacgaataaa agataatatt ctactttttg ctcccaccgc gtttgctagc acgagtgaac
     6061 accatccctc gcctgtgagt tgtacccatt cctctaaact gtagacatgg tagcttcagc
     6121 agtgttcgtt atgtacggca tcctccaaca aacagtcggt tatagtttgt cctgctcctc
     6181 tgaatcgtct ccctcgatat ttctcatccc gggccggtct ttgaaaagat aatgtatgat
     6241 tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat atacaaggtg
     6301 attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg gctagcggta
     6361 aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg tcaaacgctg
     6421 tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag tgaaagataa
     6481 atgatcgcgc gaactcgaga ccgaagggtt ttagagctag aaatagcaag ttaaaataag
     6541 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcaacataa ggagtttata
     6601 tggaaaccct tatgtttttt tgttttttat gtctccgggg tacccaattc gccctatagt
     6661 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6781 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     6841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6961 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7021 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7081 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7141 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7201 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7261 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     7321 tctgtgcggt atttcacacc gcgttttcga cactggatgg cggcgttagt atcgaatcga
     7381 cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt tctgcgcact
     7441 taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt
     7501 tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt tcttgaaaac
     7561 aagaatcttt ttattgtcag tactgattag aaaaactcat cgagcatcaa atgaaactgc
     7621 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     7681 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     7741 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     7801 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     7861 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     7921 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     7981 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     8041 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttgccgggg
     8101 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     8161 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     8221 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     8281 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     8341 gcatccatgt tggaatttaa tcgcggcctc gaaacgtgag tcttttcctt acccatggtt
     8401 gtttatgttc ggatgtgatg tgagaactgt atcctagcaa gattttaaaa ggaagtatat
     8461 gaaagaagaa cctcagtggc aaatcctaac cttttatatt tctctacagg ggcgcggcgt
     8521 ggggacaatt caacgcgtct gtgaggggag cgtttccctg ctcgcaggtc tgcagcgagg
     8581 agccgtaatt tttgcttcgc gccgtgcggc catcaaaatg tatggatgca aatgattata
     8641 catggggatg tatgggctaa atgtacgggc gacagtcaca tcatgcccct gagctgcgca
     8701 cgtca
//
