LOCUS       sequence-352564-        4202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Highly efficient in vitro transcription. Empty backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4202)
  TITLE     pIVTRup
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIVTRup
FEATURES             Location/Qualifiers
     source          1..4202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(277..293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(277..293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(290..312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    301..317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(325..331,332..349,350..355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    370..391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(508..525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(679..1267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(759..778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1438..2229,2230..2298))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2061..2080
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2316..3110)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2427..2446)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3037..3056
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(3454..3882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3564..3585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3776..3795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4008..4030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4022..4039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4023..4039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4046..4064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4136..4154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atcgctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc cttgcacctg
       61 tacctcttgg tctttgaata aagcctgagt aggaagtgag ggtctagaaa aaaaaaaaaa
      121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacgatcaat aaccgacact
      181 cctgcgtcgc ctacgcgcta cgatctgcag aattccagca cactggcggc cgttactagt
      241 ggatccgagc tcggtaccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2281 gaaatgttga atactcatac tcttcctttt tcaattcaga agaactcgtc aagaaggcga
     2341 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     2401 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     2461 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     2521 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     2581 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     2641 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     2701 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     2761 gccatgatgg atactttctc ggcaggagca aggtgggatg acaggagatc ctgccccggc
     2821 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     2881 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc
     2941 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     3001 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     3061 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     3121 cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag
     3181 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     3241 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     3301 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     3361 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     3421 ctttagcagc ccttgcgccc tgaattttgt taaaattcgc gttaaatttt tgttaaatca
     3481 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3541 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3601 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3661 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3721 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3781 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3841 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3901 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3961 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4021 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggccctct
     4081 agatgcatgc tcgagcggcc gccagtgtga tggatttgga ccctcgtaca gaagctaata
     4141 cgactcacta tagggaaata agagagaaaa gaagagtaag aagaaatata agagccaccg
     4201 at
//
