LOCUS       sequence-352567-        8785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Kwak S, Mahmud B, Dantas G
  TITLE     A Tunable and Expandable Transactivation System in Probiotic Yeast 
            Saccharomyces boulardii.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 
            10.1021/acssynbio.1c00384. Epub 2021 Dec 23.
  PUBMED    34939781
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00384"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "508-514"
COMMENT     Sequence Label: pRS42K_MCP-VP64_D-MS2
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     161..183
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(221..239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      280..1622
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    612..659
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1649..1753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1754..1822,1823..2614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1972..1991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2785..3373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3274..3293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3527..3544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3661..3682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3697..3702,3703..3720,3721..3727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3735..3751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3740..3762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3759..3775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3759..3775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3794..3814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3796..3814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4583..5287
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5334..5354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5727..5876
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        6297..6565
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     protein_bind    6567..6585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        6587..6662
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6667..6685
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      6688..6707
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     primer_bind     complement(6725..6744)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6726..6744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6754..6771)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6754..6770)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6763..6785)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6911..7366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6998..7017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7208..7229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gene            complement(join(7457..8785,1..28))
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(7457..7654)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(7660..8469)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8383..8402
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(join(8470..8785,1..28))
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 caaggagggt attctgggcc tccatgtcgc tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      121 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      181 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
      241 ttataggtta atgtcatgat aataatggtt tcttagtatg atccaatatc aaaggaaatg
      301 atagcattga aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag
      361 ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta
      421 ctagactacc tttcatccta cataaataga cgcatataag tacgcattta agcataaaca
      481 cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg
      541 acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc
      601 ggaaacgctt tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
      661 gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac
      721 cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag
      781 tatctctttg ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg
      841 ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct ctagtattac
      901 tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa acaatacgaa
      961 aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc gttcataatt
     1021 ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta tgcgcaatcc
     1081 acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac ccgcagcttc
     1141 gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag agaaaataga
     1201 caccaaagta gccttcttct aaccttaacg gacctacagt gcaaaaagtt atcaagagac
     1261 tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata
     1321 gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta tagattcttt gttggtaaaa
     1381 tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     1441 aattagcgct ctcgcgttgc atttttgttt tacaaaaatg aagcacagat tcttcgttgg
     1501 taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt
     1561 tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa aatgaagcac agatgcttcg
     1621 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3661 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3781 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcgccgggat
     3841 cgaagaaatg atggtaaatg aaataggaaa tcaaggagca tgaaggcaaa agacaaatat
     3901 aagggtcgaa cgaaaaataa agtgaaaagt gttgatatga tgtatttggc tttgcggcgc
     3961 cgaaaaaacg agtttacgca attgcacaat catgctgact ctgtggcgga cccgcgctct
     4021 tgccggcccg gcgataacgc tgggcgtgag gctgtgcccg gcggagtttt ttgcgcctgc
     4081 attttccaag gtttaccctg cgctaagggg cgagattgga gaagcaataa gaatgccggt
     4141 tggggttgcg atgatgacga ccacgacaac tggtgtcatt atttaagttg ccgaaagaac
     4201 ctgagtgcat ttgcaacatg agtatactag aagaatgagc caagacttgc gagacgcgag
     4261 tttgccggtg gtgcgaacaa tagagcgacc atgaccttga aggtgagacg cgcataaccg
     4321 ctagagtact ttgaagagga aacagcaata gggttgctac cagtataaat agacaggtac
     4381 atacaacact ggaaatggtt gtctgtttga gtacgctttc aattcatttg ggtgtgcact
     4441 ttattatgtt acaatatgga agggaacttt acacttctcc tatgcacata tattaattaa
     4501 agtccaatgc tagtagagaa ggggggtaac acccctccgc gctcttttcc gatttttttc
     4561 taaaccgtgg aatatttcgg atatcctttt gttgtttccg ggtgtacaat atggacttcc
     4621 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct
     4681 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg
     4741 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
     4801 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
     4861 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
     4921 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
     4981 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
     5041 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
     5101 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
     5161 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
     5221 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
     5281 atcaactcca agctggccgc tctagaacta gtaaaacaat gtctcttgag atgcccaaaa
     5341 agaaaagaaa agtgggtagt atggcttcta actttactca gttcgttctc gtcgacaatg
     5401 gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc taacgggatc gctgaatgga
     5461 tcagctctaa ctcgcgttca caggcttaca aagtaacctg tagcgttcgt cagagctctg
     5521 cgcagaatcg caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg cgttcgtact
     5581 taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag cttattgtta
     5641 aggcaatgca aggtctccta aaagatggaa acccgattcc ctcagcaatc gcagcaaact
     5701 ccggcatcta cggctccgga cgagccgatg ctctcgacga ttttgacctc gatatgctgg
     5761 gatccgacgc tctcgatgac ttcgaccttg acatgctggg gagtgacgct ctggacgact
     5821 tcgatctcga catgctgggc tccgacgccc tggatgactt cgatctggac atgctcatca
     5881 actgaagtgc ttttaactaa gaattattag tcttttctgc ttattttttc atcatagttt
     5941 agaacacttt atattaacga atagtttatg aatctattta ggtttaaaaa ttgatacagt
     6001 tttataagtt actttttcaa agactcgtgc tgtctattgc ataatgcact ggaaggggaa
     6061 aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag tttgaaaaat aactacatgg
     6121 atgataagaa aacatggagt acagtcactt tgagaacctt caatcagctg gtaacgtctt
     6181 cgttaattgg atactcaaaa aagatggata gcatgaatca caagatggaa ggaaatgcgg
     6241 gccacgacca cagtgatatg catatgggag atggagatga tacctgcccg ggccggtctt
     6301 tgaaaagata atgtatgatt atgctttcac tcatatttat acagaaactt gatgttttct
     6361 ttcgagtata tacaaggtga ttacatgtac gtttgaagta caactctaga ttttgtagtg
     6421 ccctcttggg ctagcggtaa aggtgcgcat tttttcacac cctacaatgt tctgttcaaa
     6481 agattttggt caaacgctgt agaagtgaaa gttggtgcgc atgtttcggc gttcgaaact
     6541 tctccgcagt gaaagataaa tgatcgtctc tatcactgat agggaggttt tagagctaga
     6601 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     6661 gcgcgcacat gaggatcacc catgtgcttt ttttgttttt tatgtctccg gggtacccaa
     6721 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6781 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6841 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6901 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6961 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7021 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7081 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7141 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7201 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7261 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7321 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     7381 ttctccttac gcatctgtgc ggtatttcac accgcgtttt cgacactgga tggcggcgtt
     7441 agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta
     7501 ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa
     7561 tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca
     7621 agttcttgaa aacaagaatc tttttattgt cagtactgat tagaaaaact catcgagcat
     7681 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     7741 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     7801 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     7861 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     7921 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     7981 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     8041 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     8101 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     8161 tgttttgccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     8221 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     8281 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     8341 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     8401 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgaaacgt gagtcttttc
     8461 cttacccatg gttgtttatg ttcggatgtg atgtgagaac tgtatcctag caagatttta
     8521 aaaggaagta tatgaaagaa gaacctcagt ggcaaatcct aaccttttat atttctctac
     8581 aggggcgcgg cgtggggaca attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag
     8641 gtctgcagcg aggagccgta atttttgctt cgcgccgtgc ggccatcaaa atgtatggat
     8701 gcaaatgatt atacatgggg atgtatgggc taaatgtacg ggcgacagtc acatcatgcc
     8761 cctgagctgc gcacgtcaag actgt
//
