LOCUS       sequence-35257-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     2-series polycistronic destination vectors
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET empty polycistronic destination vector (2D)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(259..277)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      273..320
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        387..478
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcggatccg ggccctgtac aagatcctgt aaaacgacgg ccagtgaatt cactcgagtc
       61 ccggggcgat cgcagcggat aacaatttca catcctgcag ggatatcggc gcgcccgcca
      121 gggttttccc agtcgcggcc gctcgcgcac ctggtgccat cgccctgata gacgttaatt
      181 aaagtactgg ccggccacgc gtctcgagag atccggctgc taacaaagcc cgaaaggaag
      241 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      301 gggtcttgag gggttttttg ctgaaaggag gaactatatc cgactagtag cccgcctaat
      361 gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta
      421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      541 ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca gaggaccaat tgggggcacg
      601 agtgggatac atagaactgg acttgaatag cggtaaaatc cttgagagtt ttcgccctga
      661 agagcgtttt ccaatgatga gcactttcaa agttctgcta tgtggagcag tattatcccg
      721 tgtagatgcg gggcaagagc aactcggacg acgaatacac tattcgcaga atgacttggt
      781 tgaatactcc ccagtgacag aaaagcacct tacggacgga atgacggtaa gagaattatg
      841 tagtgcc
//
