LOCUS       sequence-352571-        9105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the
            control of EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, 
            Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, 
            Fukagawa T, Shibata S, Hiraoka Y
  TITLE     Transfected plasmid DNA is incorporated into the nucleus via nuclear
            envelope reformation at telophase.
  JOURNAL   Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8.
  PUBMED    35058555
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-01-20"; volume: "5(1):78. doi"; pages: " 
            10.1038/s42003-022-03021-8"
COMMENT     Sequence Label: pAID1.2-EF1a-NmClover3-hsLem2
FEATURES             Location/Qualifiers
     source          1..9105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             24..1748
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             1749..1802
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2229..2248)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    join(2285..2859,2860..2862,2863..2871)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2452..2469)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2679..2698
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2884..2886,2887..2889,2890..3600)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2929..2950)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3537..3558
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3601..3804
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     polyA_signal    5367..5488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5404..5423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5458..5477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5642..6230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5722..5741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6457..7248,7249..7317))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    7422..7470
                     /note="synthetic polyadenylation signal"
     misc_feature    7484..7575
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7524..7543
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        7595..7805
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7617..7813
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7664..7799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7900..9078
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8131..9069
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9026..9046
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tcttgtccag aggcgctgcc accatgacat actttcctga agaggtcgtc gaacacattt
       61 ttagcttcct gcctgcacag agagatagaa acacagtgag cctggtctgc aaagtgtggt
      121 acgagatcga acgcctgagc cggagaggag tgttcgtcgg caactgctat gctgtgagag
      181 caggcagggt cgccgctagg tttccaaatg tgcgcgcact gaccgtcaag gggaaacccc
      241 acttcgccga ctttaacctg gtgccccctg attggggagg atacgccggc ccttggatcg
      301 aggcagccgc tcgcggctgt catggactgg aggaactgcg catgaagcga atggtggtct
      361 ctgacgaaag tctggagctg ctggctcgga gcttccctag gtttcgcgca ctggtgctga
      421 tttcttgcga aggcttcagc accgatggac tggcagccgt ggcctcccac tgtaagctgc
      481 tgcgggagct ggacctccag gagaatgaag tggaggatag aggccccaga tggctgtctt
      541 gcttcccaga ctcatgtacc agcctggtgt ccctgaactt tgcctgcatc aaaggcgaag
      601 tgaatgctgg gtccctggag cggctggtct caagaagccc caacctgagg tctctgcggc
      661 tgaaccggag cgtgagcgtg gacactctgg ctaagattct gctgagaacc cctaacctgg
      721 aggatctggg aaccggcaat ctgacagacg atttccagac agaatcctac tttaaactga
      781 cttctgccct ggagaagtgt aaaatgctga ggagtctgtc aggattctgg gatgcttcac
      841 ccgtgtgcct gagctttatc taccctctgt gtgcacagct gacaggcctg aacctgagct
      901 atgcaccaac cctggacgcc agtgatctga caaagatgat ctcacgctgc gtgaaactcc
      961 agcgactgtg ggtgctggac tgtatttccg ataaggggct ccaggtggtc gccagctcct
     1021 gcaaggacct ccaggagctg agagtgttcc catctgattt ttacgtggcc ggatatagtg
     1081 ctgtcactga ggaaggcctg gtggcagtct cactgggatg cccaaagctg aacagcctgc
     1141 tgtatttctg tcatcagatg actaatgctg cactggtgac cgtcgccaag aactgcccta
     1201 atttcacccg atttcggctg tgtattctgg aaccaggcaa acccgacgtg gtcacatccc
     1261 agccactgga tgaagggttt ggagctatcg tgagagagtg caagggactc cagaggctga
     1321 gcatttccgg cctgctgaca gacaaagtgt tcatgtacat cggcaagtat gctaagcagc
     1381 tggagatgct gagcattgca tttgccggag actccgataa gggcatgatg cacgtgatga
     1441 acgggtgtaa gaatctgcga aaactggaaa tccgggacag ccctttcggg gatgccgctc
     1501 tgctgggaaa ctttgccaga tacgagacaa tgaggagcct gtggatgtct agttgcaatg
     1561 tgactctgaa gggctgtcag gtcctggcta gtaaaatgcc tatgctgaac gtggaagtca
     1621 ttaatgagcg ggacgggtct aacgaaatgg aggaaaatca tggcgacctg ccaaaggtgg
     1681 agaaactgta tgtgtatcgg accaccgcag gggcaagaga tgatgctccc aactttgtga
     1741 agattctgga gggccgcggc agcctgctga cctgcggcga cgtggaggag aaccccggcc
     1801 ccatgaaaac atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga
     1861 agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa
     1921 caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg
     1981 cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg
     2041 tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt
     2101 gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg ttaatagaaa
     2161 tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa tatacccgaa
     2221 attaaggatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgaggga
     2281 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2341 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2401 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     2461 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     2521 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     2581 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     2641 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     2701 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     2761 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     2821 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgtgcgat
     2881 aacatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2941 gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg cgatgccacc
     3001 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3061 accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc cgaccacatg
     3121 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3181 tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3241 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3301 cacaagctgg agtacaactt caacagccac tacgtctata tcacggccga caagcagaag
     3361 aactgcatca aggctaactt caagatccgc cacaacgttg aggacggcag cgtgcagctc
     3421 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3481 cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     3541 gtcctgctgg agttcgtgac cgccgccggg attacacatg gcatggacga gctgtacaag
     3601 aaggagaaga gtgcttgtcc taaagatcca gccaaacctc cggccaaggc acaagttgtg
     3661 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc
     3721 ggtggcccgg aggcggcggc gttcgtgaag gtatcaatgg acggagcacc gtacttgagg
     3781 aaaatcgatt tgaggatgta taaaggagct ggtgcaggcg ctggagcggg tgccgatatg
     3841 gccggcctgt cggacctgga actgcggcgg gagctgcagg ccctgggctt ccagccagga
     3901 cccatcaccg acaccacccg ggatgtctac cgcaacaagc tgcgccgcct gcggggcgag
     3961 gcccggctgc gcgacgagga gcggctgcgg gaggaggccc ggccgcgggg cgaggagcgg
     4021 ttacgggaag aggcccggtt acgcgaggat gcgccgctgc gcgcccggcc cgccgcggcc
     4081 tctccgcggg cggagccctg gctctcccag ccggcctcgg gctcggccta cgcgacccct
     4141 ggggcctacg gtgatatccg gccctccgcg gcttcctggg tagggagccg cggcctcgcc
     4201 tatcctgccc gcccggcgca actcaggcgc cgcgcctcgg tccggggcag ctccgaggag
     4261 gacgaggacg cccggacgcc cgacagggcc acgcagggcc cgggtctcgc ggcccgccgc
     4321 tggtgggcag cgtctcccgc cccggcgcgg ctgccttcct ccctcctcgg tcccgacccg
     4381 cgcccgggcc tgcgggcgac tcgagcgggc cctgctggcg cggcgagggc ccggcctgag
     4441 gtggggcgcc ggctggagcg ctggctctct cggcttctgc tctgggccag cctagggcta
     4501 ctgctcgtct tcctgggcat cctttgggtg aagatgggca agccctcagc gccgcaggag
     4561 gcggaggaca acatgaagtt attgccagtg gactgtgaga gaaaaacaga tgagttctgt
     4621 caggccaagc agaaggcagc cttgctggag ctgctgcatg aactctacaa tttcctggcc
     4681 atccaagctg gtaattttga gtgtggaaat ccagagaatc taaaaagcaa atgcattcct
     4741 gttatggaag cccaagaata tatagccaat gtgaccagca gctcctccgc caagtttgaa
     4801 gccgcactga cctggatact gagcagtaac aaggacgtgg gcatctggtt gaaaggagaa
     4861 gaccagtctg aattggtgac gactgtggac aaggtggtct gcctggaatc tgcccacccc
     4921 cgcatgggtg ttggctgccg cctgagccgg gccttgctca ctgctgtcac caacgtgctc
     4981 atcttcttct ggtgcttggc ttttttgtgg gggctcctaa ttctcctaaa atatcggtgg
     5041 cgaaagttag aagaggagga acaagccatg tatgagatgg tgaagaagat tatagacgtg
     5101 gtccaggacc attacgtgga ctgggagcag gacatggagc gctatccata tgtaggcatc
     5161 ctgcacgtgc gcgacagctt gatccctcca cagagccgga ggcgcatgaa gcgtgtctgg
     5221 gaccgagctg tggagttcct ggcctccaac gaatcccgga tccagacgga gtcccaccgc
     5281 gttgcaggag aggacatgct ggtgtggaga tggactaagc cctcttcctt ctctgactca
     5341 gagcgataaa tccaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5461 gtttgtccaa actcatcaat gtatcttagc gctcggtcgt tcggctgcgg cgagcggtat
     5521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag cccctgcgca acgtggcagg
     6421 aagcgcgcgc tggcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     6481 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     6541 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     6601 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     6661 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     6721 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     6781 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     6841 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     6901 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     6961 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     7021 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     7081 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     7141 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     7201 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     7261 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     7321 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     7381 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     7441 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     7501 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     7561 ccagaacatt tctctggcct aaccaggctc cccagcaggc agaagtatgc aaagcatgca
     7621 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7801 aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     7861 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     7921 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     7981 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     8041 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     8101 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     8161 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     8221 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     8281 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     8341 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     8401 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     8461 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     8521 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     8581 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     8641 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     8701 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     8761 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     8821 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     8881 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     8941 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     9001 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     9061 ccatttcagg tgtcgtgaat tgcggaaggc cgtcaaggcc acgtg
//
