LOCUS       sequence-352572-       10798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre
            G, Cabantous S
  TITLE     High-content tripartite split-GFP cell-based assays to screen for 
            modulators of small GTPase activation.
  JOURNAL   J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 
            10.1242/jcs.210419.
  PUBMED    29192060
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-01-8"; volume: "131(1). pii: jcs.210419. doi"; 
            pages: " 10.1242/jcs.210419"
COMMENT     Sequence Label: pTRIP TRE-BI GFP10-RHOB/RBD-GFP11
FEATURES             Location/Qualifiers
     source          1..10798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        577..891
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    585..603
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    621..639
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    692..710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    728..746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    763..781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    799..817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     LTR             1905..2085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3613..3632)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3846..3865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3965..3987
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4025..4043)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4111..4215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4216..4284,4285..5076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4434..4453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5247..5835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5736..5755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5989..6006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6076..6096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6081..6410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6261..6396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7544..7609
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7739..7759
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8184..8318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8275..8294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8486..9119
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9166..9291
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9784..10017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10202..10246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10539..10656
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    10710..10791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
       61 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagattag gaggcgtcgg
      121 tgatgccggc ggcggtcacg tactccagca gcaccatgtg gtcgcgcttc tctccggagg
      181 acccaccacc tccagagcca ccgccaccat cgatgcctgt cttctccagc acctgggcct
      241 cctttcgccg ctgcagctca cccatgtagc tgagaatacg gctgttgcac accagcaggc
      301 tcttggtggc ttccagagcc tgctctcgct gggagcaggc tgccagcagc ttgcaggccc
      361 catccctcat ccggatctca tgatctagtt tcctctgcag ctctgtgtcc tccaggctga
      421 gtgccatctg ccggatgtag agcatattga ggtcctccag gatcatggtg gccatatgac
      481 cggtgaattc tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc
      541 caccgtacac gccacctcga cataacgcgt tcactcgagt ttactcccta tcagtgatag
      601 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
      661 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      721 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
      781 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
      841 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggaccgcg
      901 gcaccatggg cgacctgccc gacgaccact acctgtccac ccagaccatc ctgagcaagg
      961 acctgaacat cgatggtggc ggtggctctg gaggtggtgg gtcctccgga gcggccatcc
     1021 gcaagaagct ggtggtggtg ggcgacggcg cgtgtggcaa gacgtgcctg ctgatcgtgt
     1081 tcagtaagga cgagttcccc gaggtgtacg tgcccaccgt cttcgagaac tatgtggccg
     1141 acattgaggt ggacggcaag caggtggagc tggcgctgtg ggacacggcg ggccaggagg
     1201 actacgaccg cctgcggccg ctctcctacc cggacaccga cgtcattctc atgtgcttct
     1261 cggtggacag cccggactcg ctggagaaca tccccgagaa gtgggtcccc gaggtgaagc
     1321 acttctgtcc caatgtgccc atcatcctgg tggccaacaa aaaagacctg cgcagcgacg
     1381 agcatgtccg cacagagctg gcccgcatga agcaggaacc cgtgcgcacg gatgacggcc
     1441 gcgccatggc cgtgcgcatc caagcctacg actacctcga gtgctctgcc aagaccaagg
     1501 aaggcgtgcg cgaggtcttc gagacggcca cgcgcgccgc gctgcagaag cgctacggct
     1561 cccagaacgg ctgcatcaac tgctgcaagg tgctataatc tanagctagc atcgannggn
     1621 ncggatccag cggccgctcg agacctagaa aaacatggag caatcacaag tagcaataca
     1681 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     1741 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     1801 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaaaat
     1861 cgtcgagaga tgctgcatat aagcagctgc tttttgcttg tactgggtct ctctggttag
     1921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     2101 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     2161 gacattataa tagatttagc aggaattgaa ctaggagtgg agcacacagg caaagctgca
     2221 gaagtacttg gaagaagcca ccagagatac tcacgattct gcacatacct ggctaatccc
     2281 agatcctaag gattacatta agtttactaa catttatata atgatttata gtttaaagta
     2341 taaacttatc taatttacta ttctgacaga tattaattaa tcctcaaata tcataagaga
     2401 tgattactat tatccccatt taacacaaga ggaaactgag agggaaagat gttgaagtaa
     2461 ttttcccaca attacagcat ccgttagtta cgactctatg atcttctgac acaaattcca
     2521 tttactcctc accctatgac tcagtcgaat atatcaaagt tatggacatt atgctaagta
     2581 acaaattacc cttttatata gtaaatactg agtagattga gagaagaaat tgtttgcaaa
     2641 cctgaatagc ttcaagaaga agagaagtga ggataagaat aacagttgtc atttaacaag
     2701 ttttaacaag taacttggtt agaaagggat tcaaatgcat aaagcaaggg ataaattttt
     2761 ctggcaacaa gactatacaa tataacctta aatatgactt caaataattg ttggaacttg
     2821 ataaaactaa ttaaatatta ttgaagatta tcaatattat aaatgtaatt tacttttaaa
     2881 aagggaacat agaaatgtgt atcattagag tagaaaacaa tccttattat cacaatttgt
     2941 caaaacaagt ttgttattaa cacaagtaga atactgcatt caattaagtt gactgcagat
     3001 tttgtgtttt gttaaaatta gaaagagata acaacaattt gaattattga aagtaacatg
     3061 taaatagttc tacatacgtt cttttgacat cttgttcaat cattgatcga agttctttat
     3121 cttggaagaa tttgttccaa agactctgaa ataaggaaaa caatctatta tatagtctca
     3181 cacctttgtt ttacttttag tgatttcaat ttaataatgt aaatggttaa aatttattct
     3241 tctctgagat catttcacat tgcagataga aaacctgaga ctggggtaat ttttattaaa
     3301 atctaattta atctcagaaa cacatcttta ttctaacatc aatttttcca gtttgatatt
     3361 atcatataaa gtcagccttc ctcatctgca ggttccacaa caaaaatcca accaactgtg
     3421 gatcaaaaat attgggaaaa aattaaaaat agcaatacaa caataaaaaa atacaaatca
     3481 gaaaaacagc acagtataac aactttattt agcatttaca atctattagg tattataagt
     3541 aatctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     3601 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     3661 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     3721 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     3781 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     3841 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3901 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3961 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4021 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4081 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6061 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     6421 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     6481 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     6541 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     6601 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     6661 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     6721 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     6781 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     6841 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     6901 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     6961 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     7021 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     7081 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     7141 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     7201 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     7261 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     7321 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     7381 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     7441 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     7501 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     7561 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     7621 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     7681 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     7741 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     7801 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     7861 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     7921 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     7981 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     8041 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     8101 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     8161 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     8221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8281 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8341 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8401 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8461 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     8521 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8581 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8641 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8701 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8761 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8821 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8881 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9181 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9241 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9301 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9361 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9421 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9481 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9541 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9601 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9661 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9721 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9781 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9841 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9901 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9961 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10021 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10081 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10141 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10201 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10261 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10321 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10381 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10441 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    10501 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    10561 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    10621 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    10681 gatccacttt ggctgatacg cgtacgtcgg cagtgaaaaa aatgctttat ttgtgaaatt
    10741 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaaca
//
