LOCUS       sequence-352579-        8666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-LacI in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, 
            Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, 
            Fukagawa T, Shibata S, Hiraoka Y
  TITLE     Transfected plasmid DNA is incorporated into the nucleus via nuclear
            envelope reformation at telophase.
  JOURNAL   Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8.
  PUBMED    35058555
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-01-20"; volume: "5(1):78. doi"; pages: " 
            10.1038/s42003-022-03021-8"
COMMENT     Sequence Label: pGFP-LacI
FEATURES             Location/Qualifiers
     source          1..8666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             340..1134
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(394..413)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1004..1023
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1194..1214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1256..1377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1293..1312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1347..1366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2140..2174
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2665..2681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2665..2681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2678..2700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2689..2705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2713..2719,2720..2737,2738..2743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2758..2779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2896..2913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3067..3655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3147..3166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3826..4617,4618..4686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4449..4468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4687..4791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4817..5272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4954..4975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5166..5185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5399..5421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5413..5430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5414..5430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5560..5622
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        6075..6286
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6299..6567
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(6590..6592,6593..6595,6596..7306)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6590..6592,6593..6595,6596..7306)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6635..6656)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6896..6915)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7243..7264
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7362..7381)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     misc_feature    join(8407..8666,1..315,316..318,319..327)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8574..8591)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
       61 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      181 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      301 aaacacgatg ataatatggc cacaaccatg gcgtccggaa tgattgaaca agatggattg
      361 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      421 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      481 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
      541 tcgtggctgg ccgcgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
      601 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
      661 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
      721 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
      781 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
      841 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
      901 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
      961 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     1021 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     1081 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagtcgac
     1141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgcgtt aactaaactt
     1261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1381 tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctcatct ggtctccctt
     1441 ccgggggaca agacatccct gtttaatatt taaacagcag tgttcccaaa ctgggttctt
     1501 atatcccttg ctctggtcaa ccaggttgca gggtttcctg tcctcacagg aacgaagtcc
     1561 ctaaagaaac agtggcagcc aggtttagcc ccggaattga ctggattcct tttttagggc
     1621 ccattggtat ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     1681 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     1741 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     1801 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     1861 tccctgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     1921 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     1981 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     2041 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     2101 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     2161 ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct tttttccggc
     2221 tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt
     2281 cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg
     2341 ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta
     2401 acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac
     2461 agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga
     2521 actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg
     2581 aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg
     2641 gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2701 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2761 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2821 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2881 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3481 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3601 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3661 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3721 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3781 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3841 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3901 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3961 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4021 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4081 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4141 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4201 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4261 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4321 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4381 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4441 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4501 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4561 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4621 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4681 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4741 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4801 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4861 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4921 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4981 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5041 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5101 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5161 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5221 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     5281 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     5341 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     5401 cagtcacgac gttgtaaaac gacggccagt gagcgcgcct cgttcattca cgtttttgaa
     5461 cccgtggagg acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg
     5521 aagatgctcg acacgctgca gaacacgcag ctagattaac cctagaaaga taatcatatt
     5581 gtgacgtacg ttaaagataa tcatgtgtaa aattgacgca tgtgttttat cggtctgtat
     5641 atcgaggttt atttattaat ttgaatagat attaagtttt attatattta cacttacata
     5701 ctaataataa attcaacaaa caatttattt atgtttattt atttattaaa aaaaacaaaa
     5761 actcaaaatt tcttctataa agtaacaaaa cttttatgag ggacagcccc cccccaaagc
     5821 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     5881 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     5941 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     6001 tgcagacacc tggggggata cggggaaaag gcctccacgg ccaaggatct gcgatcgctc
     6061 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6121 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6181 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6241 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     6301 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     6361 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     6421 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     6481 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     6541 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacta tggtgagcaa
     6601 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6661 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6721 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6781 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6841 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6901 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6961 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     7021 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     7081 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     7141 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     7201 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     7261 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtcta gagctagcga
     7321 attcgaattt aaatcggata tggtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     7381 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     7441 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     7501 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     7561 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     7621 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     7681 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     7741 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     7801 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     7861 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     7921 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     7981 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgctaatgct
     8041 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     8101 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     8161 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     8221 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     8281 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     8341 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt aagatccgcg
     8401 gccgcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     8461 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     8521 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     8581 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     8641 aagacaaaca acgtctgtag cgaccc
//
