LOCUS       sequence-35258-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     2-series polycistronic destination vectors
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET empty polycistronic destination vector (2E)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(186..203)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(186..202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(259..278)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(260..278)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             515..706
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(700..722)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggctttgtta gcagccggat ctctcgagac gcgtggccgg ccagtacttt aattaacgtc
       61 tatcagggcg atggcaccag gtgcgcgagc ggccgcgact gggaaaaccc tggcgggcgc
      121 gccgatatcc ctgcaggatg tgaaattgtt atccgctgcg atcgccccgg gactcgagtg
      181 aattcactgg ccgtcgtttt acaggatctt gtacagggcc cggatccgag gccttctaga
      241 gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgatgcatgg
      301 tgccaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
      361 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
      421 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
      481 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagatt
//
