LOCUS       sequence-352586-       10034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing optimized Cas9 and NAT marker. Compatible with 
            CTG-clade yeast species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
  TITLE     A CRISPR/Cas9 method facilitates efficient oligo-mediated gene 
            editing in Debaryomyces hansenii.
  JOURNAL   Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 
            10.1093/synbio/ysab031. eCollection 2021.
  PUBMED    34746438
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-10-12"; volume: "6(1):ysab031. doi"; pages: " 
            10.1093/synbio/ysab031. eCollection 2021"
COMMENT     Sequence Label: pDIV488
FEATURES             Location/Qualifiers
     source          1..10034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..248
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1974..6077
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1974..6077
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6090..6110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6114..6134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6138..6158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6159..6224
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6305..6323)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      6335..6523
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    6563..6596
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6651..6994
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7573..7770
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    7833..7866
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(7959..8547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8039..8058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8718..9509,9510..9578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9341..9360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9579..9683)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(9720..10034,1..189)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
       61 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
      121 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
      181 caagcgatcc gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
      241 cacgaggccc tttgaatgcg tgcgatgggc ttagttagcc ttgcttgtga ctttgcagct
      301 ttatccatga acggatcata cgattgtatt tctgaatttg tcatagtgac tttaagttgt
      361 tctttatttg gtatgattct attttttagg actttctaaa aaatcaattt aaattttgga
      421 aaaccatttg tggtttttct taaatgattg caaaattatt aaaaaatcgt taaaaaaata
      481 ttcagtgtaa aaagactagc attcatgatc tcaacatctt tggataatat cagaatgaga
      541 aagaacagat acgcagtacg ttttttggtg agctctttgc acttctttag ttctttccat
      601 caatatcagt tttttaaact tttaggacta aaagtgatgt ttaacttcaa aatgtttaaa
      661 attttgttct tcccgacgtt cattaagaat actaatacac tttaataatt attttaatat
      721 tttgttctaa ataatgactt ttaattaaaa aagataaaat ataaaaacat cataataact
      781 caccagaggt taagaacaaa aaaacaaatt agatatctgc taatccaata tagttaaatc
      841 aatctttcct tggtataatg ggtatattac atatatttca aggaccgaca ctcctaccaa
      901 atatctaaaa tttaccatat taacataaca tgtatataaa cgtcaaatca taatcagcac
      961 tagcggaatg cgtgcgatcg cgtgcattcg gaaagcaagc aatcgtagcc ccagcaaagc
     1021 actatagtag gtgtttgctc tttttatttc ctttgctcta cgcactgtaa tacaggattg
     1081 atgaaagcag tatagaaaat cgcctcgaag cctcgtgaat cagtagatct atttacattg
     1141 ttcgtctcgt gcggtttttc accagtgtca tgagcgagcg aacttggtcg acatcattaa
     1201 aaaaaactgc ggctttcgtg tcagagtcgc tgtgtatcac ttcgacgtag gtaatcagct
     1261 cttaagcttg aagtgtggat cggaattttc ttctcctcct tcattagtca tcttccatga
     1321 aagagtgact tttgactcac acgaaggaaa atgtgcgcaa attacaaatc acctacaagc
     1381 cacaagacac attcatcaaa ggcaccaaac aagacattca agtggaccat gataaaagcc
     1441 aaggtttgaa ggcatattct gtatacttca atcttggttt gctcggccca agtcccggcg
     1501 attagcggcg cccggtggcc cggctggata gatttcaatt ggcacacgca caattatctc
     1561 cacaattgca ttgcatacac ctttacacca caactcggcg acctcggcca gcctctgaaa
     1621 acacatagcc aaaaacgaag aagggacagg cattttccct tcattgccta gtatggcata
     1681 atacgtcacc aaaccatgca ttatggattg ttaagagcta atcttcctgg tgaaaaaccg
     1741 ccacaaattg accatgacag tgcttgctcg gagttttcag cggagggcgt gcgcccgggc
     1801 aaacatagtg cattgtgatg taggcggcaa atatatgccg gatacgcgct tatggagcgg
     1861 attgccccgg cggagaaaag tatataagta gccagcgcaa ggcccccatt tctagagaat
     1921 ttttcccttc aattgattac aacttcaatt aactacaaac aaaactatca atcatggata
     1981 aaaagtatag tattggttta gatattggta ctaactctgt gggttgggca gttatcactg
     2041 acgaatataa agttccatca aagaaattta aggtgttagg taacactgac agacactcaa
     2101 taaaaaagaa tcttatcggt gctcttttgt tcgactccgg tgaaactgcc gaggctacac
     2161 gtttaaaaag aacagcaaga agaagatata cccgtagaaa aaatagaata tgttatttac
     2221 aagaaatctt ttctaatgaa atggctaaag ttgatgattc ctttttccat agattggaag
     2281 agtcattttt ggttgaagaa gacaaaaagc atgagagaca tccaatcttt gggaatatag
     2341 ttgatgaagt ggcttaccat gaaaaatatc ctaccattta tcatttaaga aagaaattgg
     2401 tagattcaac tgataaagct gaccttagat taatctattt agcacttgcc catatgatta
     2461 aatttagagg tcattttttg attgaaggtg atttgaaccc agataattct gacgtggata
     2521 aattatttat tcaattagtc caaacctaca accaattatt tgaggaaaat ccaattaatg
     2581 ctagtggtgt cgatgccaaa gctatattat cagccagatt atcaaaatct agacgtttgg
     2641 aaaatttgat tgcccaattg ccaggagaaa aaaagaatgg attatttgga aacttgatcg
     2701 cattatcatt gggtttgaca ccaaatttta aatctaattt tgatttagct gaagatgcta
     2761 aattacaatt atcaaaagac acctatgacg acgatttgga caatttactt gctcaaattg
     2821 gtgatcaata tgcagatttg ttcttagctg ctaaaaactt atctgatgct attttgttgt
     2881 ctgatatttt gagagtgaac acagaaataa ccaaagctcc attatcagca tctatgatca
     2941 aacgttatga tgaacaccat caggatttga ctttattgaa agctttggtg agacaacaat
     3001 tgccagagaa gtataaagaa atctttttcg atcaatctaa aaacgggtat gcaggttata
     3061 ttgatggggg tgcctcccaa gaggaatttt acaaatttat aaaacctatt ttagaaaaga
     3121 tggatgggac tgaggaactt ttggtcaaat tgaacagaga agatttgtta cgtaaacaga
     3181 gaacttttga taatggtagt atacctcacc aaattcattt gggtgagttg catgcaattt
     3241 taagaagaca agaagatttt tatccatttt taaaagataa tagagaaaaa atcgagaaaa
     3301 ttttaacctt tagaattcca tactatgttg ggcctttggc tagaggtaat tcaagatttg
     3361 cctggatgac acgtaaatca gaagaaacta ttaccccttg gaattttgaa gaggttgttg
     3421 ataaaggagc atcagcacag agttttattg aaagaatgac caatttcgat aaaaacttac
     3481 caaatgaaaa agttttacca aaacattcct tgttatacga atattttact gtttacaatg
     3541 aacttacaaa ggttaaatat gttactgaag gtatgcgtaa gccagccttt ttatctggag
     3601 aacagaaaaa ggcaatagtt gatttattgt ttaaaacaaa tagaaaagtt actgttaaac
     3661 aattaaaaga agattacttt aagaaaattg aatgttttga ttcagttgaa atcagtggtg
     3721 ttgaagacag atttaatgct agtttaggaa cttaccatga tttacttaaa attatcaaag
     3781 ataaagattt cttggataac gaagaaaatg aagacatttt agaagacatt gttttaacct
     3841 taactttatt cgaagataga gagatgattg aagaacgttt gaagacttat gcacatttgt
     3901 ttgacgataa agtgatgaaa cagttgaaaa gaagacgtta tactggatgg ggtagattgt
     3961 ctcgtaaatt gatcaatgga attagagata aacaaagtgg taaaactatc ttggactttt
     4021 tgaaatctga cggatttgct aatagaaatt tcatgcaatt gatccacgac gatagtttga
     4081 catttaaaga agacatccaa aaggcccaag tgagtgggca aggtgattca ttacatgaac
     4141 atattgcaaa tttagccgga tctcctgcta ttaagaaagg gatattacaa actgttaaag
     4201 ttgtggatga attagtgaaa gtaatgggaa gacataaacc tgaaaacatt gtcattgaga
     4261 tggcaagaga aaatcaaact acacaaaaag gacagaaaaa tagtagagaa cgtatgaaaa
     4321 gaatagaaga gggtattaaa gaattgggta gtcaaatatt gaaagaacac ccagtggaaa
     4381 atacccagtt gcaaaatgaa aaattatatc tttactacct tcaaaatgga cgtgatatgt
     4441 atgttgatca ggaattagat ataaatagac tttcagatta tgatgtagat catatagttc
     4501 cacaatcttt cttgaaagat gattccatag acaataaagt attaactaga agtgataaaa
     4561 atagaggtaa aagtgataat gtcccaagtg aggaagtcgt caaaaagatg aaaaattact
     4621 ggcgtcaact tttgaatgct aaattaatta ctcaaagaaa atttgataat ttgactaaag
     4681 cagaaagagg tgggctttct gaattagata aagccgggtt cattaaaaga caattggtcg
     4741 aaactagaca aattactaaa catgttgccc aaattttaga ttcccgtatg aacactaagt
     4801 atgacgaaaa tgataagtta atacgtgagg ttaaagtcat tactttaaaa tcaaaacttg
     4861 tctctgattt cagaaaggat ttccaattct ataaagttag agaaattaat aattatcatc
     4921 atgctcatga tgcatatttg aatgctgtag ttggaactgc tttaatcaag aaatacccta
     4981 aattagaatc tgaatttgta tatggtgatt acaaagtcta tgatgttaga aagatgattg
     5041 ctaaatcaga acaagaaatt ggtaaagcta cagctaaata cttcttttac tctaacatta
     5101 tgaatttctt taaaacagaa attactttgg caaacggtga aattagaaaa agacctctta
     5161 ttgaaacaaa tggtgagact ggagagatag tttgggacaa agggcgtgat ttcgctactg
     5221 ttagaaaagt tttatcaatg ccacaagtta acattgtaaa gaaaacagag gttcaaactg
     5281 gtggtttctc aaaagaaagt attttgccta aaagaaatag tgataaattg attgccagaa
     5341 aaaaggattg ggatccaaag aaatatggtg gtttcgactc accaaccgta gcctattctg
     5401 ttttggttgt ggcaaaggtt gaaaagggta aaagtaaaaa gcttaaatca gtaaaagaac
     5461 ttttgggtat tacaataatg gaaagaagtt cctttgaaaa gaaccctatt gattttttgg
     5521 aagctaaagg ttataaggaa gtaaagaagg acttaataat caaattgcct aaatattctt
     5581 tatttgaatt agaaaatggg agaaaaagaa tgttggcttc tgctggagaa ttgcaaaagg
     5641 gtaatgaatt agcattgcct tccaaatatg ttaacttctt gtatttagct tcacactatg
     5701 aaaagttgaa agggtcacca gaagataacg agcaaaaaca attatttgtt gaacaacaca
     5761 aacactactt agatgagatt atagaacaaa ttagtgaatt cagtaaaaga gtgatattag
     5821 ctgatgcaaa tttagataaa gttttgtcag cctataacaa acatagagat aagccaatta
     5881 gagaacaagc agaaaacatt attcacttat ttacccttac caatttagga gcacctgctg
     5941 ctttcaagta ttttgataca acaattgatc gtaaaagata tacctcaaca aaagaagtct
     6001 tagacgccac cttaattcat caatcaatca ctggattgta tgagacaaga attgatttgt
     6061 ctcaattggg tggtgatgaa ggggctgatc ctaagaagaa aagaaaagtt gatccaaaga
     6121 aaaagcgtaa ggtggatcct aagaaaaaga gaaaggttga ctacaaagac catgacggtg
     6181 attataaaga tcatgacatc gactacaagg atgacgatga caagtgataa tgactgcaga
     6241 gatccatcga cctgccgcca agctaattcc gggcgaattt ctgtcgagtc atgtaattag
     6301 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
     6361 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
     6421 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
     6481 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaatcgcgt gcattcaacg
     6541 ctgcaggtcg acaaccctta atataacttc gtataatgta tgctatacga agttattagg
     6601 tctagagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     6661 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     6721 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     6781 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     6841 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     6901 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     6961 acagttctca catcacatcc gaacataaac aaccatggga accaccttgg atgacactgc
     7021 ttatcgatac cgaacatctg tacctggtga tgctgaagct atcgaagccc ttgacgggtc
     7081 attcacaact gacaccgtct ttagagttac agctactggg gatgggttta cattgcgaga
     7141 agtaccagta gatcctcctt tgaccaaagt attcccagat gacgaaagtg acgatgaatc
     7201 cgatgacggt gaggacggtg acccagactc aagaacattt gtcgcttatg gagacgacgg
     7261 tgatttggcc ggtttcgtag ttgtatccta ttctggatgg aaccgaagac ttactgtcga
     7321 ggatatagag gttgccccag aacatcgtgg gcacggtgtt gggcgagccc ttatgggatt
     7381 agctacagaa tttgcccgtg aacgtggggc tggacattta tggttagaag tcaccaatgt
     7441 taatgcacca gctatacatg catatcgaag aatggggttc acactttgtg gattggatac
     7501 agctttatac gatggtacag cctcagacgg agaacaagct ttatacatgt ctatgccttg
     7561 tccataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     7621 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     7681 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtt
     7741 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     7801 gtgtcgaaaa cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga
     7861 agttattagg tgatatcaga tccactagtg gccattaaga cctgagcaaa aggccagcaa
     7921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtccttttca
     9721 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
     9781 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
     9841 ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt
     9901 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
     9961 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
    10021 atatatatgt aaag
//
