LOCUS       sequence-352587-       10804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing optimized Cas9 and NAT marker. Compatible with 
            CTG-clade yeast species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
  TITLE     A CRISPR/Cas9 method facilitates efficient oligo-mediated gene 
            editing in Debaryomyces hansenii.
  JOURNAL   Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 
            10.1093/synbio/ysab031. eCollection 2021.
  PUBMED    34746438
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-10-12"; volume: "6(1):ysab031. doi"; pages: " 
            10.1093/synbio/ysab031. eCollection 2021"
COMMENT     Sequence Label: pDIV489
FEATURES             Location/Qualifiers
     source          1..10804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1472..5575
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1472..5575
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5588..5608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5612..5632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5636..5656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5657..5722
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5803..5821)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      5833..6021
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     terminator      7630..7827
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    7890..7923
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7993..8026)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8496..8513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8496..8512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8611..8628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8782..9370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8862..8881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10283,10284..10352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10115..10134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10353..10444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10486..10505
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10511..10530
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    10737..10770
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tttaatgggc ttagttagcc ttgcttgtga ctttgcagct ttatccatga acggatcata
       61 cgattgtatt tctgaatttg tcatagtgac tttaagttgt tctttatttg gtatgattct
      121 attttttagg actttctaaa aaatcaattt aaattttgga aaaccatttg tggtttttct
      181 taaatgattg caaaattatt aaaaaatcgt taaaaaaata ttcagtgtaa aaagactagc
      241 attcatgatc tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg
      301 ttttttggtg agctctttgc acttctttag ttctttccat caatatcagt tttttaaact
      361 tttaggacta aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt
      421 cattaagaat actaatacac tttaataatt attttaatat tttgttctaa ataatgactt
      481 ttaattaaaa aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa
      541 aaaacaaatt agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg
      601 ggtatattac atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat
      661 taacataaca tgtatataaa cgtcaaatca taatcagcac tagcggaatg cgtgcgatcg
      721 cgtgcattca gcaattccaa aatcccatta gaggggttct tcacgattat ctatttgatc
      781 actctcgcct aaactataaa tatagctacc tttttatcaa tatacatgta cttccgaaca
      841 tcggccctta tctgttatta atctattaat aaacccttaa caatttcctg ttgctccttg
      901 taaaccagtc ctatatgtcc aacttgcccg gattggtctg atcttccgat ctaacaatca
      961 cgtcttacta atattataat gccttggtaa cacgtctcgc gtttacgcgt atgcccttta
     1021 ggaattttgt aaacaaactg ccccgtagat tacaaacaag agagtgaatt ccaccctcaa
     1081 ctatagacct aggagccatt tttctatata actactatct gtataaggat ggggcctcct
     1141 gggacatgtt caattgaatt ttacgcttcg aaccatacac ataacacgta aaaagctcta
     1201 atattgggct tcttaaatgg aaagttgtag tatagatttt acgcgatgat ccaattcgcg
     1261 ttttgtgtag ggaattgctt ctacatcaag caaaatttac aatgctttta tgtatacggc
     1321 cgtagacgga atagacaaca ttgtataatc aggaattgat ataaaatagt tgatatccca
     1381 ctattctaat aacttcattt tttattaatt agtatttgat aatataaaat ttcattagtt
     1441 gctttcaaac attattcaga caatctgtca tatggataaa aagtatagta ttggtttaga
     1501 tattggtact aactctgtgg gttgggcagt tatcactgac gaatataaag ttccatcaaa
     1561 gaaatttaag gtgttaggta acactgacag acactcaata aaaaagaatc ttatcggtgc
     1621 tcttttgttc gactccggtg aaactgccga ggctacacgt ttaaaaagaa cagcaagaag
     1681 aagatatacc cgtagaaaaa atagaatatg ttatttacaa gaaatctttt ctaatgaaat
     1741 ggctaaagtt gatgattcct ttttccatag attggaagag tcatttttgg ttgaagaaga
     1801 caaaaagcat gagagacatc caatctttgg gaatatagtt gatgaagtgg cttaccatga
     1861 aaaatatcct accatttatc atttaagaaa gaaattggta gattcaactg ataaagctga
     1921 ccttagatta atctatttag cacttgccca tatgattaaa tttagaggtc attttttgat
     1981 tgaaggtgat ttgaacccag ataattctga cgtggataaa ttatttattc aattagtcca
     2041 aacctacaac caattatttg aggaaaatcc aattaatgct agtggtgtcg atgccaaagc
     2101 tatattatca gccagattat caaaatctag acgtttggaa aatttgattg cccaattgcc
     2161 aggagaaaaa aagaatggat tatttggaaa cttgatcgca ttatcattgg gtttgacacc
     2221 aaattttaaa tctaattttg atttagctga agatgctaaa ttacaattat caaaagacac
     2281 ctatgacgac gatttggaca atttacttgc tcaaattggt gatcaatatg cagatttgtt
     2341 cttagctgct aaaaacttat ctgatgctat tttgttgtct gatattttga gagtgaacac
     2401 agaaataacc aaagctccat tatcagcatc tatgatcaaa cgttatgatg aacaccatca
     2461 ggatttgact ttattgaaag ctttggtgag acaacaattg ccagagaagt ataaagaaat
     2521 ctttttcgat caatctaaaa acgggtatgc aggttatatt gatgggggtg cctcccaaga
     2581 ggaattttac aaatttataa aacctatttt agaaaagatg gatgggactg aggaactttt
     2641 ggtcaaattg aacagagaag atttgttacg taaacagaga acttttgata atggtagtat
     2701 acctcaccaa attcatttgg gtgagttgca tgcaatttta agaagacaag aagattttta
     2761 tccattttta aaagataata gagaaaaaat cgagaaaatt ttaaccttta gaattccata
     2821 ctatgttggg cctttggcta gaggtaattc aagatttgcc tggatgacac gtaaatcaga
     2881 agaaactatt accccttgga attttgaaga ggttgttgat aaaggagcat cagcacagag
     2941 ttttattgaa agaatgacca atttcgataa aaacttacca aatgaaaaag ttttaccaaa
     3001 acattccttg ttatacgaat attttactgt ttacaatgaa cttacaaagg ttaaatatgt
     3061 tactgaaggt atgcgtaagc cagccttttt atctggagaa cagaaaaagg caatagttga
     3121 tttattgttt aaaacaaata gaaaagttac tgttaaacaa ttaaaagaag attactttaa
     3181 gaaaattgaa tgttttgatt cagttgaaat cagtggtgtt gaagacagat ttaatgctag
     3241 tttaggaact taccatgatt tacttaaaat tatcaaagat aaagatttct tggataacga
     3301 agaaaatgaa gacattttag aagacattgt tttaacctta actttattcg aagatagaga
     3361 gatgattgaa gaacgtttga agacttatgc acatttgttt gacgataaag tgatgaaaca
     3421 gttgaaaaga agacgttata ctggatgggg tagattgtct cgtaaattga tcaatggaat
     3481 tagagataaa caaagtggta aaactatctt ggactttttg aaatctgacg gatttgctaa
     3541 tagaaatttc atgcaattga tccacgacga tagtttgaca tttaaagaag acatccaaaa
     3601 ggcccaagtg agtgggcaag gtgattcatt acatgaacat attgcaaatt tagccggatc
     3661 tcctgctatt aagaaaggga tattacaaac tgttaaagtt gtggatgaat tagtgaaagt
     3721 aatgggaaga cataaacctg aaaacattgt cattgagatg gcaagagaaa atcaaactac
     3781 acaaaaagga cagaaaaata gtagagaacg tatgaaaaga atagaagagg gtattaaaga
     3841 attgggtagt caaatattga aagaacaccc agtggaaaat acccagttgc aaaatgaaaa
     3901 attatatctt tactaccttc aaaatggacg tgatatgtat gttgatcagg aattagatat
     3961 aaatagactt tcagattatg atgtagatca tatagttcca caatctttct tgaaagatga
     4021 ttccatagac aataaagtat taactagaag tgataaaaat agaggtaaaa gtgataatgt
     4081 cccaagtgag gaagtcgtca aaaagatgaa aaattactgg cgtcaacttt tgaatgctaa
     4141 attaattact caaagaaaat ttgataattt gactaaagca gaaagaggtg ggctttctga
     4201 attagataaa gccgggttca ttaaaagaca attggtcgaa actagacaaa ttactaaaca
     4261 tgttgcccaa attttagatt cccgtatgaa cactaagtat gacgaaaatg ataagttaat
     4321 acgtgaggtt aaagtcatta ctttaaaatc aaaacttgtc tctgatttca gaaaggattt
     4381 ccaattctat aaagttagag aaattaataa ttatcatcat gctcatgatg catatttgaa
     4441 tgctgtagtt ggaactgctt taatcaagaa ataccctaaa ttagaatctg aatttgtata
     4501 tggtgattac aaagtctatg atgttagaaa gatgattgct aaatcagaac aagaaattgg
     4561 taaagctaca gctaaatact tcttttactc taacattatg aatttcttta aaacagaaat
     4621 tactttggca aacggtgaaa ttagaaaaag acctcttatt gaaacaaatg gtgagactgg
     4681 agagatagtt tgggacaaag ggcgtgattt cgctactgtt agaaaagttt tatcaatgcc
     4741 acaagttaac attgtaaaga aaacagaggt tcaaactggt ggtttctcaa aagaaagtat
     4801 tttgcctaaa agaaatagtg ataaattgat tgccagaaaa aaggattggg atccaaagaa
     4861 atatggtggt ttcgactcac caaccgtagc ctattctgtt ttggttgtgg caaaggttga
     4921 aaagggtaaa agtaaaaagc ttaaatcagt aaaagaactt ttgggtatta caataatgga
     4981 aagaagttcc tttgaaaaga accctattga ttttttggaa gctaaaggtt ataaggaagt
     5041 aaagaaggac ttaataatca aattgcctaa atattcttta tttgaattag aaaatgggag
     5101 aaaaagaatg ttggcttctg ctggagaatt gcaaaagggt aatgaattag cattgccttc
     5161 caaatatgtt aacttcttgt atttagcttc acactatgaa aagttgaaag ggtcaccaga
     5221 agataacgag caaaaacaat tatttgttga acaacacaaa cactacttag atgagattat
     5281 agaacaaatt agtgaattca gtaaaagagt gatattagct gatgcaaatt tagataaagt
     5341 tttgtcagcc tataacaaac atagagataa gccaattaga gaacaagcag aaaacattat
     5401 tcacttattt acccttacca atttaggagc acctgctgct ttcaagtatt ttgatacaac
     5461 aattgatcgt aaaagatata cctcaacaaa agaagtctta gacgccacct taattcatca
     5521 atcaatcact ggattgtatg agacaagaat tgatttgtct caattgggtg gtgatgaagg
     5581 ggctgatcct aagaagaaaa gaaaagttga tccaaagaaa aagcgtaagg tggatcctaa
     5641 gaaaaagaga aaggttgact acaaagacca tgacggtgat tataaagatc atgacatcga
     5701 ctacaaggat gacgatgaca agtgataatg actgcagaga tccatcgacc tgccgccaag
     5761 ctaattccgg gcgaatttct gtcgagtcat gtaattagtt atgtcacgct tacattcacg
     5821 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     5881 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5941 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     6001 gaaggttttg ggacgctcga aatcgcgtgc attcaacgct gcaggtcgac aacccttaat
     6061 ataacttgga aagcaagcaa tcgtagcccc agcaaagcac tatagtaggt gtttgctctt
     6121 tttatttcct ttgctctacg cactgtaata caggattgat gaaagcagta tagaaaatcg
     6181 cctcgaagcc tcgtgaatca gtagatctat ttacattgtt cgtctcgtgc ggtttttcac
     6241 cagtgtcatg agcgagcgaa cttggtcgac atcattaaaa aaaactgcgg ctttcgtgtc
     6301 agagtcgctg tgtatcactt cgacgtaggt aatcagctct taagcttgaa gtgtggatcg
     6361 gaattttctt ctcctccttc attagtcatc ttccatgaaa gagtgacttt tgactcacac
     6421 gaaggaaaat gtgcgcaaat tacaaatcac ctacaagcca caagacacat tcatcaaagg
     6481 caccaaacaa gacattcaag tggaccatga taaaagccaa ggtttgaagg catattctgt
     6541 atacttcaat cttggtttgc tcggcccaag tcccggcgat tagcggcgcc cggtggcccg
     6601 gctggataga tttcaattgg cacacgcaca attatctcca caattgcatt gcatacacct
     6661 ttacaccaca actcggcgac ctcggccagc ctctgaaaac acatagccaa aaacgaagaa
     6721 gggacaggca ttttcccttc attgcctagt atggcataat acgtcaccaa accatgcatt
     6781 atggattgtt aagagctaat cttcctggtg aaaaaccgcc acaaattgac catgacagtg
     6841 cttgctcgga gttttcagcg gagggcgtgc gcccgggcaa acatagtgca ttgtgatgta
     6901 ggcggcaaat atatgccgga tacgcgctta tggagcggat tgccccggcg gagaaaagta
     6961 tataagtagc cagcgcaagg cccccatttc tagagaattt ttcccttcaa ttgattacaa
     7021 cttcaattaa ctacaaacaa aacaaacaac catgggaacc accttggatg acactgctta
     7081 tcgataccga acatctgtac ctggtgatgc tgaagctatc gaagcccttg acgggtcatt
     7141 cacaactgac accgtcttta gagttacagc tactggggat gggtttacat tgcgagaagt
     7201 accagtagat cctcctttga ccaaagtatt cccagatgac gaaagtgacg atgaatccga
     7261 tgacggtgag gacggtgacc cagactcaag aacatttgtc gcttatggag acgacggtga
     7321 tttggccggt ttcgtagttg tatcctattc tggatggaac cgaagactta ctgtcgagga
     7381 tatagaggtt gccccagaac atcgtgggca cggtgttggg cgagccctta tgggattagc
     7441 tacagaattt gcccgtgaac gtggggctgg acatttatgg ttagaagtca ccaatgttaa
     7501 tgcaccagct atacatgcat atcgaagaat ggggttcaca ctttgtggat tggatacagc
     7561 tttatacgat ggtacagcct cagacggaga acaagcttta tacatgtcta tgccttgtcc
     7621 ataatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
     7681 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
     7741 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgttaat
     7801 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg
     7861 tcgaaaacga gctctcgaga acccttaata taacttcgta taatgtatgc tatacgaagt
     7921 tattaggtga tatcagatcc actagtggcc attaagacct cagcgcggcc gcaaatttaa
     7981 ataaaatgaa gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa
     8041 taagggcgac acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt
     8101 gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc
     8161 tttattattt tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat
     8221 acaaaaaatc ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag
     8281 caacgtatct tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca
     8341 tatatcaagc aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact
     8401 ccccccataa aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact
     8461 cgttatcaga accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga
     8521 aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc
     8581 tggcagttcc ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9181 agagttcttg aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg
     9241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc
     9421 gcgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg
     9481 taatgctctg cttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10321 ataagggcga cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc
    10381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10441 caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt
    10501 atacctgaat atggctcata acaccccttg tttgcctggc ggcagtagcg cggtggtccc
    10561 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac
    10621 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
    10681 actgggcctt tcgcccgggc taattatggg gtgtcgccct tattcgactc tatagtgaag
    10741 ttcctattct ctagaaagta taggaacttc tgaagtgggg atttaaatgc ggccgcgctg
    10801 aggg
//
