LOCUS       sequence-352592-        5110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans mNeonGreen co-injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW77
FEATURES             Location/Qualifiers
     source          1..5110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    980..1004
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1313..1322
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1872..1896)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2666..2686)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2694..2711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2694..2710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2703..2725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3038..3060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3098..3116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3184..3288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3289..3357,3358..4149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGKEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3507..3526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4320..4908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4809..4828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5035..5057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5054..5070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5054..5070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5090..5110
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 cattttatat ctgagtagta tcctttgctt taaatgtcca taaaactaat tttataatca
       61 ataaaacaac gtttgtaaat caactgagtt tacaagtaga gacattgagg gatactttca
      121 ctatgctaaa gtgataatcg accaaataat aacttcactt tggtatttat tcctgtctta
      181 taaatgttat gtatgaatta aattcatatg catatggatc actctgacaa tttttaataa
      241 tcttccagat caatattgac taccgatgcg ggtggtcttt tgctttgaat tctgctgaac
      301 tttacacccc gaacagcaat gtgtgcttca gcctaaaaaa aagtaagtgt gttaatcagt
      361 gcccccgatt cttcattttt tgcccctctc tcccgtttcg tcggcaaaag aagagaaaat
      421 aaagataagt ctcaagatag gttggtaatc gctaaagtgg ttgtgtggat aagagtagca
      481 aaatggcagg aagagcactt tgcgcgcaca cactgtactc attgttctgg ataaaattct
      541 ctcgttgttt gccgtcggat gtctgcctct ctgcattgag ccggcttctt cactatcttt
      601 agttaaccta aaatgccgtt tcttttctcg tatcaccact atcccgttga ggttctctgc
      661 tctcttcgct ccctaccgcc agcgagcaac tatccgtggg ggcgccttgc tcggaagatg
      721 ggggggaaga aagaagattt ttgctatttg cacttgagaa agagactttt cctgcgtcga
      781 tggtcagaga acagtgtgca gacacttttc agctacctag aattacaatt ggatatcccc
      841 gcctcccaat ccacccaccc agggaaaaag aagggctcgc cgaaaatcaa agttatctcc
      901 aggctcgcgc atcccaccaa gcggttgact tctctccacc acttttcatt ttaaccctcg
      961 atcgtcagac acagaaatga caagtttgta caaaaaagca ggcttaatgg tctccaaggg
     1021 agaggaggac aacatggcct ccctcccagc cacccacgag ctccacatct tcggatccat
     1081 caacggagtc gacttcgaca tggtcggaca aggaaccgga aacccaaacg acggatacga
     1141 ggagctcaac ctcaagtcca ccaaggtaag tttaaacata tatatactaa ctaaccctga
     1201 ttatttaaat tttcagggag acctccaatt ctccccatgg atcctcgtcc cacacatcgg
     1261 atacggattc caccaatacc tcccataccc agacggaatg tccccattcc aagccgccat
     1321 ggtcgacgga tccggatacc aagtccaccg taccatgcaa ttcgaggacg gagcctccct
     1381 caccgtcaac taccgttaca cctacgaggg atcccacatc aaggtaagtt taaacagttc
     1441 ggtactaact aaccatacat atttaaattt tcagggagag gcccaagtca agggaaccgg
     1501 attcccagcc gacggaccag tcatgaccaa ctccctcacc gccgccgact ggtgccgttc
     1561 caagaagacc tacccaaacg acaaggtaag tttaaacatg attttactaa ctaactaatc
     1621 tgatttaaat tttcagacca tcatctccac cttcaagtgg tcctacacca ccggaaacgg
     1681 aaagcgttac cgttccaccg cccgtaccac ctacaccttc gccaagccaa tggccgccaa
     1741 ctacctcaag aaccaaccaa tgtacgtctt ccgtaagacc gagctcaagc actccaagac
     1801 cgagctcaac ttcaaggagt ggcaaaaggc cttcaccgac gtcatgggaa tggacgagct
     1861 ctacaagtag tacccagctt tcttgtacaa agttggtgat atctgagctc cgcatcggcc
     1921 gctgtcatca gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
     1981 aacatccata catgctattt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     2041 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     2101 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     2161 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     2221 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     2281 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
     2341 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
     2401 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg
     2461 tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
     2521 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
     2581 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
     2641 ccaatatacc aaacataact gtttccaact ttattataca tagttgataa ttcactggcc
     2701 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2761 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2821 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     2881 atgtgcggta tttcacaccg catatggtgc actctcagtg caatctgctc tgatgccgca
     2941 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3061 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3541 gggaaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgca
     3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4021 gcagcactgg ggtcagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4141 cattggtaac tgtcagacca agtttactca tatatacttt agatttattt aaaacttcat
     4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4261 taacgttagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4921 gcggcctttt tacggttcct ggtcttttgc tggccttttg ctcacatgtt ctttcctgcg
     4981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccagcgga
     5041 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatc aactttgtat
     5101 agaaaagttg
//
