LOCUS       sequence-35260-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     4-series pFastBac LIC vectors (for insect cell expression)
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac LIC cloning vector (4A)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    339..473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(376..395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     430..449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  502..667
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cagttttgta ataaaaaaac ctataactat tccggattat tcataccgtc ccaccatcgg
       61 gcgcggatcc ttaattaagt acttccaatc caatattgga agtggataac cggtccgaag
      121 cgcgcggaat tcaaaggcct acgtcgacga gctcactagt cgcggccgct ttcgaatcta
      181 gagcctgcag tctcgaggca tgcggtacca agcttgtcga gaagtactag aggatcataa
      241 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      301 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      421 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
      481 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
      541 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
      601 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
      661 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
      721 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
      781 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
      841 ggcgaatggg acgcgccctg tagcggcgca tta
//
