LOCUS       sequence-35261-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     4-series pFastBac LIC vectors (for insect cell expression)
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac His6 TEV LIC cloning vector (4B)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..128
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    335..469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(372..391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     426..445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  498..663
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cagttttgta ataaaaaaac ctataactat tccggattat tcataccgtc ccaccatcgg
       61 gcgcggatcc cggtccgatg ggttcttctc accatcacca tcaccatgaa aacctgtact
      121 tccaatccaa tattggaagt ggataacgga tccgaattcg agcgccgtcg acaagcttgc
      181 ggccgcactc gaggcatgcg gtaccaagct tgtcgagaag tactagagga tcataatcag
      241 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      301 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      421 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
      481 tgagcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
      541 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
      601 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
      661 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
      721 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
      781 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatgg
//
