LOCUS       sequence-352619-        6129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER protein alpha-1-antitrypsin (WT), myc tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos 
            JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
  TITLE     ER-misfolded proteins become sequestered with mitochondria and 
            impair mitochondrial function.
  JOURNAL   Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w.
  PUBMED    34857875
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-2"; volume: "4(1):1350. doi"; pages: " 
            10.1038/s42003-021-02873-w"
COMMENT     Sequence Label: pAT-WT-Myc
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..1129
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             1108..1116
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    1153..1201
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1357..1374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1528..2116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1608..1627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2316..3107,3108..3176))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3281..3329
                     /note="synthetic polyadenylation signal"
     misc_feature    3343..3434
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3383..3402
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        3585..3964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3965..4168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4119..4139
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4165..4189
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5457..5486
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    5518..5639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5529..5548)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5583..5602
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        join(5833..6129,1..61)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5833..6024
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5856..5876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(6041..6129,1..47)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6103..6122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
       61 aaagctcgat tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag
      121 cgttgaaaaa tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga
      181 gggcgaagag agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt
      241 gaatagctgc gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc
      301 tgcactaccc atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg
      361 catcagtaga cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc
      421 tgtgttacag cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac
      481 cagcggcttc ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt
      541 catttgcgcc attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt
      601 gtccgccagc gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga
      661 ggtgcgccac ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg
      721 catcaccgcc gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc
      781 caacatcttc ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga
      841 gcgccggcat cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg
      901 cctggatcag ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca
      961 aggccgctgc gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc
     1021 tcgccggagc gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa
     1081 ccgccggccc agtacacgac cgcgcgctaa ggaggtaggt cgagtttaaa ctctagaacc
     1141 ggtcatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     1201 gttcgaacta gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1261 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1321 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2281 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
     2341 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
     2401 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
     2461 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
     2521 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
     2581 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
     2641 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
     2701 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
     2761 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
     2821 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
     2881 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
     2941 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
     3001 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
     3061 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
     3121 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
     3181 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa
     3241 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc
     3301 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
     3361 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
     3421 cagaacattt ctctggccta actggcctca atattggcca ttagccatat tattcattgg
     3481 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     3541 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
     3601 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3841 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3901 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3961 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
     4021 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4081 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
     4141 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatca ctagaagctt
     4201 cgatgccgtc ttctgtctcg tggggcatcc tcctgctggc aggcctgtgc tgcctggtcc
     4261 ctgtctccct ggctgaggat ccccagggag atgctgccca gaagacagat acatcccacc
     4321 atgatcagga tcacccaacc ttcaacaaga tcacccccaa cctggctgag ttcgccttca
     4381 gcctataccg ccagctggca caccagtcca acagcaccaa tatcttcttc tccccagtga
     4441 gcatcgctac agcctttgca atgctctccc tggggaccaa ggctgacact cacgatgaaa
     4501 tcctggaggg cctgaatttc aacctcacgg agattccgga ggctcagatc catgaaggct
     4561 tccaggaact cctccgtacc ctcaaccagc cagacagcca gctccagctg accaccggca
     4621 atggcctgtt cctcagcgag ggcctgaagc tagtggataa gtttttggag gatgttaaaa
     4681 agttgtacca ctcagaagcc ttcactgtca acttcgggga caccgaagag gccaagaaac
     4741 agatcaacga ttacgtggag aagggtactc aagggaaaat tgtggatttg gtcaaggagc
     4801 ttgacagaga cacagttttt gctctggtga attacatctt ctttaaaggc aaatgggaga
     4861 gaccctttga agtcaaggac accgaggaag aggacttcca cgtggaccag gtgaccaccg
     4921 tgaaggtgcc tatgatgaag cgtttaggca tgtttaacat ccagcactgt aagaagctgt
     4981 ccagctgggt gctgctgatg aaatacctgg gcaatgccac cgccatcttc ttcctgcctg
     5041 atgaggggaa actacagcac ctggaaaatg aactcaccca cgatatcatc accaagttcc
     5101 tggaaaatga agacagaagg tctgccagct tacatttacc caaactgtcc attactggaa
     5161 cctatgatct gaagagcgtc ctgggtcaac tgggcatcac taaggtcttc agcaatgggg
     5221 ctgacctctc cggggtcaca gaggaggcac ccctgaagct ctccaaggcc gtgcataagg
     5281 ctgtgctgac catcgacgag aaagggactg aagctgctgg ggccatgttt ttagaggcca
     5341 tacccatgtc tatccccccc gaggtcaagt tcaacaaacc ctttgtcttc ttaatgattg
     5401 aacaaaatac caagtctccc ctcttcatgg gaaaagtggt gaatcccacc caaaaagaac
     5461 aaaaactcat ctcagaagag gatctgtaat aaggccggcc gcttcgagca gacatgataa
     5521 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     5581 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5641 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     5701 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     5761 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     5821 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     5881 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5941 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6001 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6121 ggccgcctc
//
