LOCUS       sequence-352622-        7817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DM Firefly luciferase reporter fused to a KAR2 signal sequence for 
            ER targeting and HDEL for ER retention and fused to eGFP at the 
            C-terminus in yeast plasmid with URA marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos 
            JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
  TITLE     ER-misfolded proteins become sequestered with mitochondria and 
            impair mitochondrial function.
  JOURNAL   Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w.
  PUBMED    34857875
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-2"; volume: "4(1):1350. doi"; pages: " 
            10.1038/s42003-021-02873-w"
COMMENT     Sequence Label: p406 – TDH3-ER-FlucDM-GFP- Ura
FEATURES             Location/Qualifiers
     source          1..7817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..173
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(250..266)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..267)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      269..516
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(285..303)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(534..553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(535..553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(563..580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(563..579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(572..594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      720..1175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(807..826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1017..1038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1306..2109)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2110..2325)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(2349..2368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2468..2490
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2528..2546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2614..2718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2719..2787,2788..3579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2937..2956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3750..4338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4239..4258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4492..4509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4626..4647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4662..4667,4668..4685,4686..4692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4700..4716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4705..4727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4724..4740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4724..4740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4759..4779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4761..4779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4804..5447
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5341..5364
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5633..7279
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDQDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFQVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             join(7316..7318,7319..7321,7322..7817,1..215)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7316..7318,7319..7321,7322..7817,1..215)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7361..7382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7622..7641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
       61 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      121 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      181 ccgggatcac tctcggcatg gacgagctgt acaagaaaaa gacccacgac gaattgtagt
      241 aaagcttatc gataccgtcg acctcgagtc atgtaattag ttatgtcacg cttacattca
      301 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
      361 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
      421 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
      481 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata
      541 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      601 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      661 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
      721 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      781 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      901 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1021 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1081 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1141 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     1201 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
     1261 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     1321 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     1381 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
     1441 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
     1501 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
     1561 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
     1621 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
     1681 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
     1741 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
     1801 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
     1861 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
     1921 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
     1981 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
     2041 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
     2101 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa
     2161 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
     2221 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg
     2281 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt ggtatggtgc
     2341 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     2401 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2461 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     2521 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     2581 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2641 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2701 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2761 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2821 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2881 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2941 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3001 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3061 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3121 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3181 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3241 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3301 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3361 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3421 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3481 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3541 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3601 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3661 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4381 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4441 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4501 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4561 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4621 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4681 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4741 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctcagt
     4801 ttatcattat caatactcgc catttcaaag aatacgtaaa taattaatag tagtgatttt
     4861 cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca
     4921 aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat
     4981 tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa
     5041 gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct
     5101 tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga
     5161 gtgatgcaac ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta
     5221 tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa
     5281 aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga
     5341 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
     5401 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg attctagaac
     5461 tagtatgttt ttcaacagac taagcgctgg caagctgctg gtaccactct ccgtggtcct
     5521 gtacgccctt ttcgtggtaa tattaccttt acagaattct ttccactcct ccaatgtttt
     5581 agttagaggt ttggatcctg acttaggtac tacttattcc ttgctagctg tggaagatgc
     5641 caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga
     5701 gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga
     5761 cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc
     5821 agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa
     5881 tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc
     5941 agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac
     6001 cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc
     6061 gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat
     6121 gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga
     6181 gagcttcgac caggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt
     6241 gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga
     6301 ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca
     6361 ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc aagtcgtgct
     6421 catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc
     6481 tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta
     6541 cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg
     6601 tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga
     6661 aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa
     6721 ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt
     6781 gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa
     6841 ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc
     6901 ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata
     6961 caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat
     7021 cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt
     7081 cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag
     7141 ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa
     7201 aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa
     7261 gggcggcaag atcgccgtga tccccgggat cggtggtggt tctggaggcg gttctatggt
     7321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7501 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     7561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     7801 caaggtgaac ttcaaga
//
