LOCUS       sequence-352623-        9557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Myorg-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9557)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV CDHblast OST-Myorg-V5
FEATURES             Location/Qualifiers
     source          1..9557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4306..4347
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4458..4669
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4682..4950
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4982..5380
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5404..5992
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5457..5477)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5875..5886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6066..6299
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6371..6492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6408..6427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6462..6481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6532..6667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6700..6716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6700..6716)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6713..6735)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6724..6740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6748..6754,6755..6772,6773..6778))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6793..6814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6931..6948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7102..7690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7861..8652,8653..8721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8484..8503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8722..8826)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8894..8912
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8950..8972)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9072..9091
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9285..9307
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9299..9316
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9300..9316
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9331..9557
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagagctagc gaattgggat ccagtaccct tcaccatgtc ccagaacctt
     2101 caggagacaa gccaggccta cccccgacat cgacctggta gccacgcagg ccctaaaagt
     2161 ctcaaagtta ccccacgagc aaccatgtac actttcctgc ctgacaattt ttcacctgcc
     2221 aaacccaagc ccactaaaga gctgagacct ctgctgtgct ccgcggtact gggactgcta
     2281 ctggtgttgg ccgcagttgt ggcctggtgc tactacagtg cctccctacg caaagctgaa
     2341 cgcctgcgcg cggagctgct ggacctgaac cgtggcggct tctccatccg taaccagaag
     2401 ggtgagcaag tcttccgctt ggccttccgt tccggtgctc tagaccttga ctcctgcagc
     2461 cgggatggtg cgctgcttgg ctgctcccga gcggccgacg gacgcccgtt gcacttcttt
     2521 attcagacag tacgacctaa ggacactgta atgtgctacc gcgtgcgctg ggaggaagct
     2581 gtgccagggc gtgctgtgga gcacgcgatg tttctgggag atgctgcggc gcactggtat
     2641 ggcggcgcag agatgaggac gcaacactgg cccatccgcc tagatggcca gcaagaaccg
     2701 cagccatttg tcacgagcga cgtctattca tctgacgccg cattcggggg catccttgag
     2761 cgctactggt tatcttcacg cgccgctgcc atcaaagtca atgattcagt gcccttccac
     2821 ctgggctgga atagcacgga gcgttcgatg agactgcagg cacgctacca cgacacatct
     2881 tacaagccac cagctggccg tactgccgcg ccagagctca gctaccgcgt gtgcgtgggc
     2941 tcagatgtca cttccatcca caagtacatg gttcgacgtt acttcaacaa gccatccagg
     3001 gtaccagcgt cagaggcctt ccgagacccc atttggtcca cgtgggcact gcatgggcgc
     3061 gctgtggacc agaacaaggt gctgcaattc gcccagcaga tccgccagca ccgctttaat
     3121 agcagccacc tggaaatcga tgacatgtac acgcccgcct atggcgactt taatttcgac
     3181 gagggcaagt ttcccaacgc cagtgacatg ttccgccgcc tgcgagatgc cggcttccgc
     3241 gtcacacttt gggtgcatcc gtttgtcaac tataactcgt cgagcttcgg cgaaggtgtg
     3301 gagcgcgagc tgttcgtgcg cgagcccacg ggccggctgc ccgcgctggt gcgctggtgg
     3361 aacggtattg gcgcagtgct ggacttcacg cacccagagg cccgagagtg gttccaggga
     3421 cacctacggc gcctgcgctt gcgctacaac gtgacctcct ttaagttcga cgcgggtgag
     3481 gtcagctacc tgcccaggga cttcagcacc tacaggcctc tgtctgaccc cagtgtgtgg
     3541 agcaggcggt acaccgagat ggcggagccc ttcttctcgc tagccgaggt ccgcgtgggg
     3601 taccagtcac agaacatctc ctgcttcttc cgtctagtgg accgcgactc ggtatggggc
     3661 tacgacctgg ggctgcgctc tcttatccct gcggtgctca ccgtcagtat gctgggctat
     3721 ccgttcatct tgcccgatat gataggtggc aacgcggtgc cggagcgcac agccggccgc
     3781 caagatgggc cggggccaga gcgcgagctc tacgtgcgct ggctggaggt ggccgccttc
     3841 atgcctgcca tgcagttttc aatccctcct tggcagtacg acgcagaagt ggtagccatc
     3901 gcacacaaat tcgctgctct gcgcgcctcc ctcgtggcgc cactgctgct agagcttgct
     3961 ggtgagatca ccgacactgg cgaccccatc gtgcgccccc tgtggtggat cgcaccgggg
     4021 gatgaaacgg cccatcgcat tgactcacag tttctcatcg gggacacgct gttggtggcg
     4081 ccggtgctag agcctggtaa gcaagagcgt gatgtctacc tgcctgccgg caaatggcga
     4141 agctacaagg gtgaactttt tgacaagacg ccggtgctgc tcacggatta ccctgtcgac
     4201 ctggatgagg tcgcctactt tacctgggcg tccaagggtc aagacaattc tgcagatatc
     4261 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     4321 aaccctctcc tcggtctcga ttctacgcgt accggtgtat acgcggccgc taaatagttg
     4381 aatttaaata gatctccagc acagtggtaa atagttgatt cgaacaagga tctgcgatcg
     4441 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4501 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4561 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4621 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     4681 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     4741 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     4801 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     4861 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     4921 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgcccatggg
     4981 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     5041 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     5101 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     5161 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     5221 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     5281 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     5341 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcgcgtc
     5401 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5461 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5521 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5581 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5641 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5701 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5761 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5821 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5881 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5941 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     6001 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6061 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     6121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6301 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6361 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6481 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     6541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6601 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6661 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     6721 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6781 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6841 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6901 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7081 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7621 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7681 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7741 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7801 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7861 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7921 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7981 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8041 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8101 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8161 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8221 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8281 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8341 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8401 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8461 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8521 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8581 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8641 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8701 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8761 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8821 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8881 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8941 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9001 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9061 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9121 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     9181 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     9241 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     9301 taaaacgacg gccagtgcca agctgacgcg tgtagtctta tgcaatactc ttgtagtctt
     9361 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     9421 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     9481 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     9541 tagctcgata cataaac
//
