LOCUS       sequence-352625-       11055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-551b-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-551b-3p
FEATURES             Location/Qualifiers
     source          1..11055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    1196..1220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1237..1929
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(1952..1976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2261..2316
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2700..2725
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    3397..3417
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3466..4644
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3697..4635
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4661..4685
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4690..4699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4696..4698,4699..4701,4702..5415)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4741..4762)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(5417..5441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5596..5651
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6012..6028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6012..6028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6025..6047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6036..6052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6060..6066,6067..6084,6085..6090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6105..6126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6340..6359)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6351..7160
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6418..6437)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(7412..8000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7492..7511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8171..8962,8963..9031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8794..8813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9032..9103)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9120..9157
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             9188..9787
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9188..9207)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9684..9704
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9953..10008
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    10987..11007
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag aagggcgaat tcgacccaag
     1201 tttgtacaaa aaagcaggct ggatccaggt gccaccatgg tgagcgagct gattaaggag
     1261 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1321 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag
     1381 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1441 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc
     1501 ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc tacccaggac
     1561 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1621 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacactg
     1681 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1741 ggccacctga tctgcaacct taagaccaca tacagatcca agaaacccgc taagaacctc
     1801 aagatgcccg gcgtctacta tgtggacagg agactggaaa gaatcaagga ggccgacaaa
     1861 gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1921 ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa agtggtacgt
     1981 ctcaagacca gaggtctctg cttctcgcgt gctgaaacca agtatgggtc gcctgaaacc
     2041 aagtatgggt cgcctgaaac caagtatggg tcgcctgaaa ccaagtatgg gtcgccaacc
     2101 agaagagacc tgtgcttcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     2161 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     2221 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     2281 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2341 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     2401 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     2461 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     2521 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     2581 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     2641 tcccgagcgg tgcgttttta tgcttgtagt attgtataat gtttttaaga tccaagcttc
     2701 tgacctcttc tcttcctccc acagggcctc gagacaattg catcgatggt accagtgaaa
     2761 gtctgcaatg aattcgagtt ggcgcgcctg tcattctaaa tctctctttc agcctaaagc
     2821 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     2881 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     2941 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     3001 cgggggaggg acgtaattac atccctgggg gctttgggag ggggctgtcc ccgtgagctc
     3061 ccaggcgcgc ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc
     3121 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     3181 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     3241 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     3301 tacatccctg ggggctttgg gggggggctg tccccgtgag ctcccaggcg cgccaactcg
     3361 gtaccgtatc gatgtcgacg ttaacgctag tgatatcaac tttgtataga aaagttggct
     3421 ccgaattcgc ccttgacagc agagatccag tttggttaat taattggctc cggtgcccgt
     3481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tgtggggagg ggtcggcaat
     3541 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3661 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3721 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3781 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3841 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3901 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3961 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     4021 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     4081 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     4141 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     4201 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     4261 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     4321 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     4381 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     4441 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     4501 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4561 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4621 tttcttccat ttcaggtgtc gtgagaaggg cgaattcgac ccaagtttgt acaaaaaagc
     4681 aggctgaccg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4741 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg cgagggcgag
     4801 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4861 gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt cgcccgctac
     4921 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4981 gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc
     5041 gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc
     5101 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta tatcatggcc
     5161 gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt ggaggacggc
     5221 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5281 ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag
     5341 cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct cggcatggac
     5401 gagctgtaca agtaataccc agctttcttg tacaaagtgg tacgcgtgaa ttcactcctc
     5461 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     5521 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     5581 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     5641 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     5701 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     5761 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     5821 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     5881 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     5941 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     6001 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6061 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     6181 atcgacagta ctaagcttgg tctaataccc aatctctcgt cttatccaga tgttttatac
     6241 gcctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     6301 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     6361 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     6421 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     6481 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     6541 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     6601 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     6661 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     6721 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     6781 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     6841 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     6901 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     6961 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     7021 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     7081 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     7141 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     7201 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     7261 cgttccactg agcgtcagag gtatcactag tattacagag gtaagttata acagtcgcct
     7321 aacccaattg aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8281 catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat
     8341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9061 tttatcaagg ttattgtctc atgagcggat acatatttga atgtaccgaa tttctcgagg
     9121 gcttgtcgac gacggcggtc tccgtcgtca ggatcatgaa ttcgaccgcc tgtctcaagg
     9181 tgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     9241 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9301 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9361 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     9421 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9481 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9541 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9601 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9661 cttcctggag acatccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9721 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9781 tgcctgagct ttgagactac gcgtgaattc actcctcagg tgcaggctgc ctatcagaag
     9841 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     9901 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     9961 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    10021 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    10081 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    10141 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    10201 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    10261 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    10321 ctcttatgga gatccctcga cctgcagccc aagcttgaat tctctagaat taccctgtta
    10381 tccctatgct tggtttaccg agctcttatt ggttttcaaa cttcattgac tgtgcccaac
    10441 gcgcctggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
    10501 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
    10561 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
    10621 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
    10681 gggaaaaagc taggctgaaa gaccggttct tagaatgaca cgcgcctggg agctcacggg
    10741 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
    10801 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
    10861 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
    10921 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag ctaacttctg
    10981 agacgacaac tttgtataga aaagttggct ccgaattcgc ccttgacagc agagatccag
    11041 tttggttaat taatt
//
