LOCUS       sequence-35263-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     5-series pFastBac Dual LIC vectors (for insect cell expression)
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac Dual LIC cloning vector (5A)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    325..459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(362..381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     416..435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  488..653
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gttttgtaat aaaaaaacct ataactattc cggattattc ataccgtccc accatcgggc
       61 gcggatcctt aattaagtac ttccaatcca atattggaag tggataaccg gtccgaagcg
      121 cgcggaattc aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga
      181 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
      241 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      301 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      421 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
      481 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
      541 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
      601 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
      661 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
      721 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
      781 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
      841 gccctgtagc
//
