LOCUS       sequence-352630-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      438..1026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     927..946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1153..1158,1159..1175,1176..1181)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1229..2419
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1581..1600)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(join(2593..2603,1..9))
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(join(2594..2603,1..9))
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
       61 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca aagctggtct
      121 cggattgcta gtaatattgg tggcaaacag gctcttgaga cggttcagcg cctccttcca
      181 gttctctgtc aagcccacgg actcacccca gatcaagttg tagcgattgc tagtaatatt
      241 ggtggcaaac aggctcttga gacggttcag cgcctccttc cagttctctg tcaagcccac
      301 ggactcaccc cagatcaagt tgtagcgata gcctgagacc atatctggtc gagccaccca
      361 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
      421 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      481 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      541 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      601 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      661 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      721 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      781 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      841 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      901 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      961 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1021 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1081 cacatgttct ttcctgcgtt atccacagaa tcaggggata acgcaggaaa gaacatgaat
     1141 taattctcat gtttgacagc ttatcatcga ttagctttaa tgcggtagtt tatcacagtt
     1201 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     1261 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     1321 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     1381 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     1441 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     1501 gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc
     1561 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     1621 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     1681 gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg tgctcaacgg
     1741 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgacc
     1801 catgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     1861 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     1921 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     1981 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     2041 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     2101 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     2161 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     2221 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     2281 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     2341 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     2401 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     2461 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttgatcg
     2521 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     2581 aaataaaata tcccctatag tga
//
