LOCUS       sequence-352631-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..23)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5..23)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(28..44)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..44)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      452..1040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     941..960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1167..1172,1173..1189,1190..1195)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1243..2433
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1595..1614)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
       61 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
      121 accaaagctg gtctcgagcc agtaatattg gtggcaaaca ggctcttgag acggttcagc
      181 gcctccttcc agttctctgt caagcccacg gactcacccc agatcaagtt gtagcgattg
      241 ctagtaatat tggtggcaaa caggctcttg agacggttca gcgcctcctt ccagttctct
      301 gtcaagccca cggactcacc ccagatcaag ttgtagcgat tgcaagttga gaccatatct
      361 ggtcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
      421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1081 gctggccttt tgctcacatg ttctttcctg cgttatccac agaatcaggg gataacgcag
     1141 gaaagaacat gaattaattc tcatgtttga cagcttatca tcgattagct ttaatgcggt
     1201 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     1261 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     1321 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     1381 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     1441 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     1501 tacgcgatca tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcc
     1561 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     1621 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     1681 ggccccgtgg ccgggggact gttgggcgcc atctccttac atgcaccatt ccttgcggcg
     1741 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     1801 ggagagcgcc gacccatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     1861 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     1921 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     1981 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     2041 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     2101 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     2161 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     2221 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     2281 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     2341 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     2401 cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     2461 gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     2521 ccaacccttg atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     2581 gcctgattgg gagaaaataa aat
//
