LOCUS       sequence-352632-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      380..968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     869..888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1095..1100,1101..1117,1118..1123)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1171..2361
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1523..1542)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2535..2554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2536..2554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2559..2575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2559..2575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta gaccaaagct
       61 ggtctcgaag taatattggt ggcaaacagg ctcttgagac ggttcagcgc ctccttccag
      121 ttctctgtca agcccacgga ctcaccccag atcaagttgt agcgattgct agtaatattg
      181 gtggcaaaca ggctcttgag acggttcagc gcctccttcc agttctctgt caagcccacg
      241 gactcacccc agatcaagtt gtagcgattg cttcctgaga ccatatctgg tcgagccacc
      301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
      361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1021 ctcacatgtt ctttcctgcg ttatccacag aatcagggga taacgcagga aagaacatga
     1081 attaattctc atgtttgaca gcttatcatc gattagcttt aatgcggtag tttatcacag
     1141 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
     1201 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
     1261 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
     1321 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
     1381 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
     1441 gcgaccacac ccgtcctgtg gattctctac gccggacgca tcgtggccgg catcaccggc
     1501 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     1561 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     1621 gggggactgt tgggcgccat ctccttacat gcaccattcc ttgcggcggc ggtgctcaac
     1681 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgccga
     1741 cccatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1801 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1861 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     1921 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     1981 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     2041 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     2101 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     2161 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
     2221 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
     2281 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     2341 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
     2401 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttgat
     2461 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     2521 gaaaataaaa tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2581 tctggcccgt gtctcaaaat
//
