LOCUS       sequence-352638-       12948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, 
            Kan and Amp selection markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Jones KR, Belvin BR, Macrina FL, Lewis JP
  TITLE     Sequence and characterization of shuttle vectors for molecular 
            cloning in Porphyromonas, Bacteroides and related bacteria.
  JOURNAL   Mol Oral Microbiol. 2020 Aug;35(4):181-191. doi: 10.1111/omi.12304. 
            Epub 2020 Jul 9.
  PUBMED    32592236
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/omi.12304";
            journalName: "Mol Oral Microbiol"; date: "2020-08"; volume: "35"; 
            issue: "4"; pages: "181-191"
COMMENT     Sequence Label: pG106 - KA
FEATURES             Location/Qualifiers
     source          1..12948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             342..665
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    359..415
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(416..432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(641..660)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     oriT            912..1021
                     /note="incP origin of transfer"
     oriT            1169..1278
                     /note="incP origin of transfer"
     rep_origin      3855..4443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             9111..9119
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(9506..9525)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9507..9525)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9532..9548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9689..10117
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9776..9795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9986..10007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             10461..11255
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10515..10534)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11125..11144
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(11273..11341,11342..12133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11491..11510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12304..12892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg aattcactgg
      421 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      481 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      541 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      601 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      661 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      721 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagtcca tcactcctct
      781 aggcgaagcc catatcagca gagctaagac gtcgtctgga aggcagtaca ccttgatagg
      841 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
      901 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
      961 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     1021 cggctgacgc cgttggatac accaaggaaa gtctacacga acccgacgtc gtctggaagg
     1081 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     1141 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     1201 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     1261 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     1321 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1381 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1441 aattcgagct cggtaccccc gatagcttcc gctattgctt ttttgctcat cggtatttgc
     1501 aacatcatag aaattgcata cctttgttcc tcggttatat gtttgctcat ctgcaacttt
     1561 tttttctttg gacggacaat taaagcaaag atagcaaact ttatccattc agagtgagag
     1621 aaagggggac attgtctctc tttcctctct gaaaaataaa tgtttttatt gcttattatc
     1681 cgcacccaaa aagttgcatt tataagttga actcaagaag tattcacctg taagaagtta
     1741 ctaatgacaa aaaagaaatt gcccgttcgt tttacgggtc agcactttac tattgataaa
     1801 gtgctaataa aagatgcaat aagacaagca aatataagta atcaggatac ggttttagat
     1861 attggggcag gcaaggggtt tcttactgtt catttattaa aaatcgccaa caatgttgtt
     1921 gctattgaaa acgacacagc tttggttgaa catttacgaa aattattttc tgatgcccga
     1981 aatgttcaag ttgtcggttg tgattttagg aattttgcag ttccgaaatt tcctttcaaa
     2041 gtggtgtcaa atattcctta tggcattact tccgatattt tcaaaatcct gatgtttgag
     2101 agtcttggaa attttctggg aggttccatt gtccttcaat tagaacctac acaaaagtta
     2161 ttttcgagga agctttacaa tccatatacc gttttctatc atactttttt tgatttgaaa
     2221 cttgtctatg aggtaggtcc tgaaagtttc ttgccaccgc caactgtcaa atcagccctg
     2281 ttaaacatta aaagaaaaca cttatttttt gattttaagt ttaaagccaa atacttagca
     2341 tttatttcct gtctgttaga gaaacctgat ttatctgtaa aaacagcttt aaagtcgatt
     2401 ttcaggaaaa gtcaggtcag gtcaatttcg gaaaaattcg gtttaaacct taatgctcaa
     2461 attgtttgtt tgtctccaag tcaatggtta aactgttttt tggaaatgct ggaagttgtc
     2521 cctgaaaaat ttcatccttc gtagttcaaa gtcgggtggt tgtcaagatg atttttttgg
     2581 tttggtgtcg tcttttttta agctgccgca taacggctgg caaattggcg atggagcgga
     2641 aacgtaaaag aagttatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg
     2701 cagtatctta aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta
     2761 attaagaagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
     2821 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
     2881 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
     2941 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
     3001 tactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
     3061 gtataaaatt gttgggagta ttccttacca tttaagcaca caaattatta aaaaagtggt
     3121 ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
     3181 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
     3241 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
     3301 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
     3361 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
     3421 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
     3481 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
     3541 ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata ctactgacag
     3601 cttcggggat cgagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3961 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4501 atgtgatcct ctattgcaga tggtcttgtt gcacacccct ctgaagggtt ctatatcatc
     4561 tataccacct cttcagagga gtttctcacg ttatctgaag aagattattc tctgttcgct
     4621 tcttttgcca atttggttgt tcgagaggac tccaattcaa atggcttaag aagcgcaaaa
     4681 ccctctgggg atggctggaa atttggaggt aaaggaaagg ggaaactaga tgccatccac
     4741 atcggctatg aattatcgaa aatcctagga gaaaatcaag attttgagat ctatgtagaa
     4801 tacaatggcg atgggtctta taccgtttgg tatagggaag tccaaaaatc gtctaaaggg
     4861 agtaatccgt aaagactttt aatgtcctca taagagccaa cgaaagagag gggcatacgt
     4921 ctctctcttt caacgtatgc ttgaacgatt gcttactcga tgtgattaaa tagagctggc
     4981 ttgataggtc agctctattt ctgtttttcg ttttgaaaat atgataggca aataccatgg
     5041 atatgacata acagaaatag agctactaag cccaaagcca cgaaccataa cgatgtcaga
     5101 ggatcacgtg gcgctactta gtagctctat ctcacttgca aagttactaa aagagatctg
     5161 cgtgcgagcc taagcgtgag aggacgataa gctcctcgtc tgcgcttatc tgctctatcc
     5221 atatcaagag caggtcaccc tcaatgtgta gctccataca gccctcatag ttccctataa
     5281 gcttgtgagg acgcatttct aagggtatct ccacaccctt agacagcatt tcaatgacat
     5341 ctacgatttt agagcgcctt ttgaggtcgt tcctaaatcg cttaaggtct ttcttaaact
     5401 tggtagtata taccagccta cgcttcctca tttccaaaca aagacgcttc tattgtttct
     5461 cgggtagttg tatccacaga gccggcatag cgaccgctct tagcgtctat gcaggcctct
     5521 gccgtctcgg cattataagg tctatacccc attcggtaga gtaaggtctc aatatagttg
     5581 cttaggcttc tattctcagc ccttgcttca ctgcgtatct gctggagtac actagcatca
     5641 attctaattg acgtggatgc ttttagctca atattatcca tttctattat attctacttg
     5701 attatattca ctgcaaatat agacaaatct cccaaataaa cgtctgtcgg ctgggcgtca
     5761 gttcacgcac caaatgtgtg tgtaatggga gcgacaagaa gttgtccaat aaaatcgttc
     5821 tccgtaactt tgggcaggcg ctagctacga ccatagttat agcacattat aactacatac
     5881 gtagttgtgt gctctccgag gggcatggag gacaccccca gcacccccta aagttgaccc
     5941 aaaagtacaa gcgatgagca aaacaagtat ccatatcgag ccgtgtaaaa tcggcagtag
     6001 tgagcaacat aatcaacgct tgaagcatct tgattatgtg cgacccgacc tctccccaca
     6061 aaacgagagt tgggttggcg tagcagattt gcccgcgcat cttgaacaac tccgaatact
     6121 tgtaaaggag aagacgggca gagcgatgca acagaaagca accccgataa gagagggtgt
     6181 aatagtaatc aatcaaggca cctcgattaa ccaattaagg ggtctcgcaa cggcaataga
     6241 actgcgatgg ggtattaaga cgctacagat atacacccac aaggacgagg ggcatataga
     6301 tagcgatgac acgtggaagc caaacctaca tgcccatatg gtgcttgatt gggtgaacca
     6361 cgacacgggc aagagtatca agcttagcaa gcaagatatg gcggagatgc agacgatggt
     6421 ggcggactgc ctgcaaatgg ttaggggaga gagtagcgat gtcaaacacc tcggggctat
     6481 acaatacaag aatcaagccg aagagctgcg tctccaaacc cttaaagaac aaacgatgaa
     6541 agaagaacag gctcgggagc tggcactagc cgaagctaag cggatggagc aggttgcgtc
     6601 tgagcagatt atcgcagaga ataagaagct tggagggctg tggatggact ggcaaaaatc
     6661 atttgaggcc tctgagaagc gccgagagag cataacggct cgtaagatag aggaggataa
     6721 gctatgggaa gaacttgggt tgaaaggagc ctttaaatcg ttttgggagg aatgcaagga
     6781 ttggtttaga ggtttcggcg atgggctcaa agacttagct ctgctgatat gggcttcgcc
     6841 tagaggagtg atggacaaag acgtgaggtg cacagcggat cggaagaggg ggaaattact
     6901 gctcaatggg catacactag agcaggtaag agcacccaag atagttgtac caagctcccc
     6961 catacaaagg caggcgcaat ccgtcagtga gtgcgctacg aggcaggcta gagatacaga
     7021 cagacgcaga gagaagcgtg ttgaccaaga gagacgagag cgcaggggtc gcaagatttg
     7081 accgcactat gttcttaaag cgtttcccaa gggctttgcc accacttgcg gacggtcggt
     7141 ctacttagtc ctgtctctct cgcacaacgg ctcttgttgt tgctgtttgg gtttctatcc
     7201 ctccactcct gcaccttggc gtattgcggg ctgtcttccg ctagtacgat agagcctttt
     7261 ggtcttccgt taccctctct ccaatctttt cttccctttg cttcctgcaa aatatctcta
     7321 gagcctcttg cgagctttag atgtaagtct tgcggtctcc cattgcgttt gttaggttct
     7381 attcttagcc ccgacctatc ctctatcagc tggcgagggt atgtctctga gctctcctta
     7441 tatgctttta gagcgtctag agcgtcctgc acggagaagt gcctgcccgt gttgcccgtt
     7501 agtgcttcca ttcgtggcac aagctctaga gcgtcccgtt tgagtatgtc aaatggcacg
     7561 ttacacttct tggcatatac tgctaagaac ataatgcagt ggtagcgatg cccaacagcg
     7621 accttttcgt tcgtccacag catatgtagc caccactcat acagccccct atgcacatgc
     7681 cacgtcttac gaggtgcgcc ctctacgatg cgtttttgat accaatctgg gtatagctct
     7741 ttagctcttg cttttgttag cctattcgtg gagtatgccc ctttgtccaa cgcttgcgcc
     7801 tgctcctgcg acacccccga gttaaggtac ttcagtgtag gcttgctagc aaaccgattc
     7861 agctcctcaa tagtgtgtag tggggcatca gcattgtgcc aagctgttac aatgtccccg
     7921 tgcttcgtac gactgcctgg caccctaaac ggctgtataa tgggctgtat ttgcacttgg
     7981 ttgtcctccc ccgtcctaga ggacgctttg ttccatataa gccgtgtaag agctttttta
     8041 atgttggtga gcgtctcgta caccttgggg tataagggca tcggcttagc aagtatgtag
     8101 tacaagtgga tgccatcgcc cgagttgacg atgatattag cttgtggggt catccctatc
     8161 gtctgctgat ggattacctc tgacatctct ggcactccga cctcatcaag gtcaaaggcg
     8221 agggcataca gatagcgggc attgctcttg aacctcctgc gccccacgta ggtggctggg
     8281 gttgttacca caaacttgta ccgccccatc agctcctcta agctatccca accacacacc
     8341 ttgatgtcat cctcgcttgt ggcatcgccc actaccaccc acgtcttaga cctcctgcca
     8401 ttatcattag ccctgcctgc atcaggctct acgctgtaga cagcttgcac caaagcatta
     8461 tagcggaaag gttgagctgg attatagatt gctttcttac ctgctttgga cttagacttt
     8521 ctccctgctt gttgagccct ccaatcgatg tattcttggc acagctcttt ttcaaaagga
     8581 tagatagggc attgcgggtc attatggtca caagaaccaa agattatttg gaagaactca
     8641 aagggtttaa gagatcgata ttcaaaggtc tcattactct ctaagtataa atttttagag
     8701 agaaaaacat cgtgagaaat attagaatta tcagtcatcg ttctacgtca ttttggggtg
     8761 attttttggt gtcgcttcgc tcccccaaaa aatcatacgg ggggcatata ttatcaatat
     8821 attttttttg ccacttgtgg cacaaagata aggaggattt ttgagacctc cttctacgtc
     8881 attttggggt gattttttgg tgtcgcttcg ctcccccaaa aaatcatacg gggggcatat
     8941 attatcaata tatttttttt tgccacttgt ggcacaaaga taaggaggat ttttgagacc
     9001 tccttctacg tcattttggg tggaattttt ggctcgctac gctccccaaa aattcatacg
     9061 ggggggcgta tattatcaat gaagtaaaat ccccacttgt ggtacaaagg taaggaggtt
     9121 ttttgagacc tccttctgcg tcatcgttct acgtcatttt ggggttgatt ttttggggaa
     9181 gaaacaaaga atatcgcaac aagtaggtat tgcatatatt ttttgcaatt ctttatcttt
     9241 gcggtaagac aacctcttaa gagagactta tcttgaatac aaaacactaa acctaagcag
     9301 ctatgaaaac tagaacattc tgcgcaatag caatcgggct attgtctttg gcgttaactt
     9361 cgtgccaaaa caaccttgac ttgttaaagc caagccaaga cactgaacca gaacaaatca
     9421 ctcaattaca tgtgcgcaag ccgaattctg cagatatcca tcacactggc ggccgctcga
     9481 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
     9541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9661 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     9721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
    10141 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
    10201 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
    10261 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
    10321 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
    10381 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
    10441 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
    10501 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    10561 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    10621 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    10681 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    10741 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
    10801 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    10861 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    10921 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    10981 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    11041 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    11101 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    11161 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    11221 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
    11281 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    11341 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    11401 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    11461 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    11521 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    11581 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    11641 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    11701 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    11761 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    11821 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11881 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11941 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    12001 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    12061 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    12121 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    12181 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    12481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    12541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    12601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    12661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12841 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12901 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctca
//
