LOCUS       sequence-35264-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     5-series pFastBac Dual LIC vectors (for insect cell expression)
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac Dual His6 TEV LIC cloning vector (5B)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             106..126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    352..486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(389..408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     443..462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  515..680
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gttttgtaat aaaaaaacct ataactattc cggattattc ataccgtccc accatcgggc
       61 gcggatcccg gtccgatggg ttcttctcac catcaccatc accatgaaaa cctgtacttc
      121 caatccaata ttggaagtgg ataacggatc cgaattcaaa ggcctacgtc gacgagctca
      181 ctagtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt cgagaagtac
      241 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      301 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
      481 tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca
      541 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
      601 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
      661 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
      721 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
      781 aaatttttct gtcatctctt cgttattaat gtttgtaatt gact
//
