LOCUS       sequence-352646-        4494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A4_STOP
FEATURES             Location/Qualifiers
     source          1..4494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2051..2150)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2167..2186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2168..2186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2191..2207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2191..2207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2309..2328)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2320..3129
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2387..2406)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3276..3864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3765..3784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4018..4035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4194..4221)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4313..4399)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4448..4470
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4462..4479
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4463..4479
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc ctctctggtg
      121 agcctggagc tgggactgct gctggccgtg ctggtggtga ccgcaacagc aagcccacct
      181 gcaggactgc tgtctctgct gacaagcgga cagggcgccc tggatcagga ggccctggga
      241 ggactgctga acaccctggc agacagggtg cactgcgcaa atggaccctg cggcaagtgt
      301 ctgtctgtgg aggatgccct gggcctgggc gagccagagg gaagcggact gccaccagga
      361 cctgtgctgg aggcaagata cgtggcaagg ctgtctgccg cagccgtgct gtatctgagc
      421 aaccctgagg gcacctgtga ggatgcaaga gcaggactgt gggcatccca cgcagaccac
      481 ctgctggccc tgctggagtc cccaaaggcc ctgacacccg gcctgtcttg gctgctgcag
      541 aggatgcagg caagagcagc aggacagacc ccaaagatgg catgcgtgga catcccccag
      601 ctgctggagg aggccgtggg agcaggagca ccaggaagcg ccggaggcgt gctggccgcc
      661 ctgctggatc acgtgcggtc tggcagctgt ttccacgccc tgccatcccc ccagtacttt
      721 gtggacttcg tgtttcagca gcacagctcc gaggtgccaa tgaccctggc agagctgagc
      781 gccctgatgc agaggctggg agtgggaagg gaggcacact ccgatcactc tcacaggcac
      841 aggggagcat ctagcaggga ccctgtgcca ctgatctcct ctagcaattc ctctagcgtg
      901 tgggatacag tgtgcctgtc cgccagagac gtgatggcag catatggact gtctgagcag
      961 gcaggagtga cccctgaggc atgggcccag ctgagcccag ccctgctgca gcagcagctg
     1021 tccggagcat gtacatccca gtctaggcct ccagtgcagg atcagctgag ccagtccgag
     1081 cggtacctgt atggctctct ggccaccctg ctgatctgcc tgtgcgccgt gttcggactg
     1141 ctgctgctga cctgcacagg ctgtagaggc gtgacccact acatcctgca gacatttctg
     1201 tccctggcag tgggagcagt gaccggcgac gccgtgctgc acctgacacc caaggtgctg
     1261 ggactgcaca cccacagcga ggagggactg tccccacagc ctacatggag gctgctggca
     1321 atgctggcag gactgtatgc cttctttctg ttcgagaacc tgtttaatct gctgctgcca
     1381 agggaccctg aggatctgga ggacggccct tgcggacact cctctcactc ccacggagga
     1441 cactcccacg gcgtgtctct gcagctggca ccttctgagc tgaggcagcc aaagccacct
     1501 cacgagggat ctagagccga tctggtggcc gaggagagcc cagagctgct gaacccagag
     1561 ccacggagac tgagcccaga gctgaggctg ctgccttaca tgatcaccct gggcgatgcc
     1621 gtgcacaatt tcgcagacgg actggcagtg ggagcagcct ttgccagctc ctggaagacc
     1681 ggactggcca catccctggc cgtgttctgc cacgagctgc cacacgagct gggcgacttt
     1741 gccgccctgc tgcacgcagg actgagcgtg cgccaggccc tgctgctgaa cctggcatcc
     1801 gccctgaccg ccttcgccgg cctgtacgtg gccctggccg tgggcgtgag cgaggagtcc
     1861 gaggcatgga tcctggcagt ggcaacaggc ctgttcctgt atgtggccct gtgcgatatg
     1921 ctgcctgcca tgctgaaggt gcgggaccct agaccatggc tgctgtttct gctgcacaat
     1981 gtgggactgc tgggaggatg gacagtgctg ctgctgctga gcctgtatga ggacgatatc
     2041 accttttaag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2101 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2161 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2221 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     2281 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2341 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2401 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2461 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2521 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2581 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2641 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2701 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2761 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2821 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2881 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2941 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3001 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3061 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3121 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3181 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4141 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4201 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4261 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4321 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     4381 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4441 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
