LOCUS       sequence-352647-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Soriano P.
  TITLE     Generalized lacZ expression with the ROSA26 Cre reporter strain.
  JOURNAL   Nat Genet. 1999 Jan;21(1):70-1.
  PUBMED    9916792
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "1999-01"; volume: "21(1)"; pages: "70-1"
COMMENT     Sequence Label: pROSA26-1
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    626..1274
                     /label=HA-L
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    1075..5415
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        5465..5964
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5500..5523)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5897..5916
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5991..6563
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          6640..6705
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(6773..6790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6779..7003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7065..7085)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7065..7083)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7104..7120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7104..7120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7117..7139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7128..7144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7152..7158,7159..7176,7177..7182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7197..7218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7335..7352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7506..8094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7586..7605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8265..9056,9057..9125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8888..8907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9126..9230)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9257..9712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9344..9363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9554..9575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9838..9860
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9852..9869
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9853..9869
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9879..9898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9879..9897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9916..9920,1..1078)
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
ORIGIN
        1 aggccctccg agcgtggtgg agccgttctg tgagacagcc gggtacgagt cgtgacgctg
       61 gaaggggcaa gcgggtggtg ggcaggaatg cggtccgccc tgcagcaacc ggagggggag
      121 ggagaaggga gcggaaaagt ctccaccgga cgcggccatg gctcgggggg gggggggcag
      181 cggaggagcg cttccggccg acgtctcgtc gctgattggc ttcttttcct cccgccgtgt
      241 gtgaaaacac aaatggcgtg ttttggttgg cgtaaggcgc ctgtcagtta acggcagccg
      301 gagtgcgcag ccgccggcag cctcgctctg cccactgggt ggggcgggag gtaggtgggg
      361 tgaggcgagc tggacgtgcg ggcgcggtcg gcctctggcg gggcggggga ggggagggag
      421 ggtcagcgaa agtagctcgc gcgcgagcgg ccgcccaccc tccccttcct ctgggggagt
      481 cgttttaccc gccgccggcc gggcctcgtc gtctgattgg ctctcggggc ccagaaaact
      541 ggcccttgcc attggctcgt gttcgtgcaa gttgagtcca tccgccggcc agcgggggcg
      601 gcgaggaggc gctcccaggt tccggccctc ccctcggccc cgcgccgcag agtctggccg
      661 cgcgcccctg cgcaacgtgg caggaagcgc gcgctggggg cggggacggg cagtagggct
      721 gagcggctgc ggggcgggtg caagcacgtt tccgacttga gttgcctcaa gaggggcgtg
      781 ctgagccaga cctccatcgc gcactccggg gagtggaggg aaggagcgag ggctcagttg
      841 ggctgttttg gaggcaggaa gcacttgctc tcccaaagtc gctctgagtt gttatcagta
      901 agggagctgc agtggagtag gcggggagaa ggccgcaccc ttctccggag gggggagggg
      961 agtgttgcaa tacctttctg ggagttctct gctgcctcct ggcttctgag gaccgccctg
     1021 ggcctgggag aatcccttcc ccctcttccc tcgtgatctg caactccagt ctttctagaa
     1081 gatgggcggg agtcttctgg gcaggcttaa aggctaacct ggtgtgtggg cgttgtcctg
     1141 caggggaatt gaacaggtgt aaaattggag ggacaagact tcccacagat tttcggtttt
     1201 gtcgggaagt tttttaatag gggcaaataa ggaaaatggg aggataggta gtcatctggg
     1261 gttttatgca gcaaaactac aggttattat tgcttgtgat ccgcctcgga gtattttcca
     1321 tcgaggtaga ttaaagacat gctcacccga gttttatact ctcctgcttg agatccttac
     1381 tacagtatga aattacagtg tcgcgagtta gactatgtaa gcagaatttt aatcattttt
     1441 aaagagccca gtacttcata tccatttctc ccgctccttc tgcagcctta tcaaaaggta
     1501 ttttagaaca ctcattttag ccccattttc atttattata ctggcttatc caacccctag
     1561 acagagcatt ggcattttcc ctttcctgat cttagaagtc tgatgactca tgaaaccaga
     1621 cagattagtt acatacacca caaatcgagg ctgtagctgg ggcctcaaca ctgcagttct
     1681 tttataactc cttagtacac tttttgttga tcctttgcct tgatccttaa ttttcagtgt
     1741 ctatcacctc tcccgtcagg tggtgttcca catttgggcc tattctcagt ccagggagtt
     1801 ttacaacaat agatgtattg agaatccaac ctaaagctta actttccact cccatgaatg
     1861 cctctctcct ttttctccat ttataaactg agctattaac cattaatggt ttccaggtgg
     1921 atgtctcctc ccccaatatt acctgatgta tcttacatat tgccaggctg atattttaag
     1981 acattaaaag gtatatttca ttattgagcc acatggtatt gattactgct tactaaaatt
     2041 ttgtcattgt acacatctgt aaaaggtggt tccttttgga atgcaaagtt caggtgtttg
     2101 ttgtctttcc tgacctaagg tcttgtgagc ttgtattttt tctatttaag cagtgctttc
     2161 tcttggactg gcttgactca tggcattcta cacgttattg ctggtctaaa tgtgattttg
     2221 ccaagcttct tcaggaccta taattttgct tgacttgtag ccaaacacaa gtaaaatgat
     2281 taagcaacaa atgtatttgt gaagcttggt ttttaggttg ttgtgttgtg tgtgcttgtg
     2341 ctctataata atactatcca ggggctggag aggtggctcg gagttcaaga gcacagactg
     2401 ctcttccaga agtcctgagt tcaattccca gcaaccacat ggtggctcac aaccatctgt
     2461 aatgggatct gatgccctct tctggtgtgt ctgaagacca caagtgtatt cacattaaat
     2521 aaataaatcc tccttcttct tctttttttt ttttttaaag agaatactgt ctccagtaga
     2581 atttactgaa gtaatgaaat actttgtgtt tgttccaata tggtagccaa taatcaaatt
     2641 actctttaag cactggaaat gttaccaagg aactaatttt tatttgaagt gtaactgtgg
     2701 acagaggagc cataactgca gacttgtggg atacagaaga ccaatgcaga ctttaatgtc
     2761 ttttctctta cactaagcaa taaagaaata aaaattgaac ttctagtatc ctatttgttt
     2821 aaactgctag ctttacttaa cttttgtgct tcatctatac aaagctgaaa gctaagtctg
     2881 cagccattac taaacatgaa agcaagtaat gataattttg gatttcaaaa atgtagggcc
     2941 agagtttagc cagccagtgg tggtgcttgc ctttatgcct ttaatcccag cactctggag
     3001 gcagagacag gcagatctct gagtttgagc ccagcctggt ctacacatca agttctatct
     3061 aggatagcca ggaatacaca cagaaaccct gttggggagg ggggctctga gatttcataa
     3121 aattataatt gaagcattcc ctaatgagcc actatggatg tggctaaatc cgtctacctt
     3181 tctgatgaga tttgggtatt attttttctg tctctgctgt tggttgggtc ttttgacact
     3241 gtgggctttc tttaaagcct ccttcctgcc atgtggtctc ttgtttgcta ctaacttccc
     3301 atggcttaaa tggcatggct ttttgccttc taagggcagc tgctgagatt tgcagcctga
     3361 tttccagggt ggggttggga aatctttcaa acactaaaat tgtcctttaa tttttttttt
     3421 aaaaaatggg ttatataata aacctcataa aatagttatg aggagtgagg tggactaata
     3481 ttaaatgagt ccctccccta taaaagagct attaaggctt tttgtcttat acttaacttt
     3541 ttttttaaat gtggtatctt tagaaccaag ggtcttagag ttttagtata cagaaactgt
     3601 tgcatcgctt aatcagattt tctagtttca aatccagaga atccaaattc ttcacagcca
     3661 aagtcaaatt aagaatttct gacttttaat gttaatttgc ttactgtgaa tataaaaatg
     3721 atagcttttc ctgaggcagg gtctcactat gtatctctgc ctgatctgca acaagatatg
     3781 tagactaaag ttctgcctgc ttttgtctcc tgaatactaa ggttaaaatg tagtaatact
     3841 tttggaactt gcaggtcaga ttcttttata ggggacacac taagggagct tgggtgatag
     3901 ttggtaaaat gtgtttcaag tgatgaaaac ttgaattatt atcaccgcaa cctacttttt
     3961 aaaaaaaaaa gccaggcctg ttagagcatg cttaagggat ccctaggact tgctgagcac
     4021 acaagagtag ttacttggca ggctcctggt gagagcatat ttcaaaaaac aaggcagaca
     4081 accaagaaac tacagttaag gttacctgtc tttaaaccat ctgcatatac acagggatat
     4141 taaaatattc caaataatat ttcattcaag ttttccccca tcaaattggg acatggattt
     4201 ctccggtgaa taggcagagt tggaaactaa acaaatgttg gttttgtgat ttgtgaaatt
     4261 gttttcaagt gatagttaaa gcccatgaga tacagaacaa agctgctatt tcgaggtctc
     4321 ttggtttata ctcagaagca cttctttggg tttccctgca ctatcctgat catgtgctag
     4381 gcctacctta ggctgattgt tgttcaaata aacttaagtt tcctgtcagg tgatgtcata
     4441 tgatttcata tatcaaggca aaacatgtta tatatgttaa acatttgtac ttaatgtgaa
     4501 agttaggtct ttgtgggttt gatttttaat tttcaaaacc tgagctaaat aagtcatttt
     4561 tacatgtctt acatttggtg gaattgtata attgtggttt gcaggcaaga ctctctgacc
     4621 tagtaaccct acctatagag cactttgctg ggtcacaagt ctaggagtca agcatttcac
     4681 cttgaagttg agacgttttg ttagtgtata ctagtttata tgttggagga catgtttatc
     4741 cagaagatat tcaggactat ttttgactgg gctaaggaat tgattctgat tagcactgtt
     4801 agtgagcatt gagtggcctt taggcttgaa ttggagtcac ttgtatatct caaataatgc
     4861 tggccttttt taaaaagccc ttgttcttta tcaccctgtt ttctacataa tttttgttca
     4921 aagaaatact tgtttggatc tccttttgac aacaatagca tgttttcaag ccatattttt
     4981 tttccttttt tttttttttt ttggtttttc gagacagggt ttctctgtat agccctggct
     5041 gtcctggaac tcactttgta gaccaggctg gcctcgaact cagaaatccg cctgcctctg
     5101 cctcctgagt gccgggatta aaggcgtgca ccaccacgcc tggctaagtt ggatattttg
     5161 ttatataact ataaccaata ctaactccac tgggtggatt tttaattcag tcagtagtct
     5221 taagtggtct ttattggccc ttcattaaaa tctactgttc actctaacag aggctgttgg
     5281 tactagtggc acttaagcaa cttcctacgg atatactagc agattaaggg tcagggatag
     5341 aaactagtct agcgttttgt atacctacca gctttatact accttgttct gatagaaata
     5401 tttcaggaca tctagcttat cgataccgtc gacggtatcg ataagcttga tatcgaattc
     5461 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5521 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5581 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5641 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5701 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5761 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     5821 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5881 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5941 tcttcctcat ctccgggcct ttcgacctgc aggtcctcgc catggatcct gatgatgttg
     6001 ttgattcttc taaatctttt gtgatggaaa acttttcttc gtaccacggg actaaacctg
     6061 gttatgtaga ttccattcaa aaaggtatac aaaagccaaa atctggtaca caaggaaatt
     6121 atgacgatga ttggaaaggg ttttatagta ccgacaataa atacgacgct gcgggatact
     6181 ctgtagataa tgaaaacccg ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc
     6241 caggactgac gaaggttctc gcactaaaag tggataatgc cgaaactatt aagaaagagt
     6301 taggtttaag tctcactgaa ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa
     6361 ggttcggtga tggtgcttcg cgtgtagtgc tcagccttcc cttcgctgag gggagttcta
     6421 gcgttgaata tattaataac tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta
     6481 attttgaaac ccgtggaaaa cgtggccaag atgcgatgta tgagtatatg gctcaagcct
     6541 gtgcaggaaa tcgtgtcagg cgatctcttt gtgaaggaac cttacttctg tggtgtgaca
     6601 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     6661 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     6721 actgatgaat gggagcagtg gtggaatgca gatcctagag ctcgctgatc agcctcgact
     6781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7021 accagctggg gctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta
     7081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9241 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9481 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9541 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9601 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9661 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc
     9721 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9781 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9841 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     9901 aattggagct ccaccgcggc
//
