LOCUS       sequence-352648-        5046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A10_STOP
FEATURES             Location/Qualifiers
     source          1..5046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2603..2702)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2719..2738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2720..2738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2743..2759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2743..2759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2861..2880)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2872..3681
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2939..2958)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3828..4416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4317..4336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4570..4587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4746..4773)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4865..4951)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5000..5022
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5014..5031
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5015..5031
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa ggtgcacatg
      121 cacaccaagt tctgcctgat ctgtctgctg acattcatct ttcaccactg caaccactgt
      181 cacgaggagc acgatcacgg accagaggcc ctgcacaggc agcacagagg catgaccgag
      241 ctggagccct ctaagttcag caagcaggcc gccgagaacg agaagaagta ctatatcgag
      301 aagctgtttg agcggtacgg cgagaatggc agactgagct tctttggcct ggagaagctg
      361 ctgaccaacc tgggcctggg cgagaggaag gtggtggaga tcaatcacga ggatctgggc
      421 cacgaccacg tgtcccacct ggacatcctg gccgtgcagg agggcaagca ctttcactcc
      481 cacaaccacc agcactctca caaccacctg aatagcgaga accagaccgt gacaagcgtg
      541 tccaccaagc gcaatcacaa gtgcgatccc gagaaggaga cagtggaggt gtccgtgaag
      601 tctgacgata agcacatgca cgaccacaac caccggctga gacaccacca caggctgcac
      661 caccacctgg atcacaacaa tacccaccac ttccacaatg actctatcac acctagcgag
      721 cgcggcgagc cttccaatga gccatctacc gagacaaaca agacccagga gcagtccgat
      781 gtgaagctgc caaagggcaa gcggaagaag aagggccgca agagcaacga gaattccgaa
      841 gtgatcacac ccggctttcc ccctaatcac gatcagggcg agcagtatga gcacaacagg
      901 gtgcacaagc ccgaccgcgt gcacaatcct ggacactccc acgtgcacct gccagagagg
      961 aacggacacg acccaggaag aggacaccag gacctggatc ctgacaatga gggcgagctg
     1021 aggcacacca ggaagaggga ggcaccacac gtgaagaaca atgccatcat cagcctgaga
     1081 aaggatctga acgaggacga tcaccaccac gagtgtctga atgtgacaca gctgctgaag
     1141 tactatggac acggagcaaa cagcccaatc tccaccgacc tgttcacata cctgtgccct
     1201 gccctgctgt atcagatcga ttcccggctg tgcatcgagc actttgacaa gctgctggtg
     1261 gaggatatca ataaggacaa gaacctggtg ccagaggacg aggcaaacat cggagcatct
     1321 gcctggatct gcggcatcat cagcatcacc gtgatctctc tgctgagcct gctgggcgtg
     1381 atcctggtgc ctatcatcaa tcagggctgt ttcaagtttc tgctgacctt cctggtggcc
     1441 ctggcagtgg gaacaatgag cggcgatgca ctgctgcacc tgctgccaca ctcccaggga
     1501 ggacatgacc actctcacca gcacgcacac ggacacggac actctcacgg acacgagagc
     1561 aacaagttcc tggaggagta cgatgccgtg ctgaagggac tggtggccct gggaggcatc
     1621 tatctgctgt tcatcatcga gcactgcatc aggatgttta agcactacaa gcagcagcgc
     1681 ggcaagcaga agtggtttat gaagcagaat accgaggaga gcacaatcgg ccggaagctg
     1741 tccgaccaca agctgaacaa tacccctgat tctgactggc tgcagctgaa gccactggcc
     1801 ggcacagacg attccgtggt gtctgaggat agactgaacg agaccgagct gacagacctg
     1861 gagggacagc aggagagccc accaaagaat tacctgtgca tcgaggagga gaagatcatc
     1921 gatcactctc acagcgacgg cctgcacacc atccacgagc acgatctgca cgcagcagca
     1981 cacaatcacc acggagagaa caagacagtg ctgagaaagc acaaccacca gtggcaccac
     2041 aagcacagcc accactccca cggaccatgc cactccggat ctgacctgaa ggagaccggc
     2101 atcgccaaca tcgcctggat ggtcatcatg ggcgatggca tccacaattt ctccgacgga
     2161 ctggcaatcg gagcagcctt ttctgccgga ctgaccggag gaatcagcac atccatcgcc
     2221 gtgttctgtc acgagctgcc tcacgagctg ggcgattttg ccgtgctgct gaaggccggc
     2281 atgaccgtga agcaggccat cgtgtacaat ctgctgagcg ccatgatggc ctatatcggc
     2341 atgctgatcg gcacagccgt gggccagtac gccaacaata tcaccctgtg gatcttcgcc
     2401 gtgacagccg gcatgtttct gtatgtggcc ctggtggaca tgctgcccga gatgctgcac
     2461 ggcgatggcg acaacgagga gcacggcttc tgccctgtgg gccagtttat cctgcagaat
     2521 ctgggcctgc tgttcggctt tgccatcatg ctggtcatcg ccctgtacga ggataagatc
     2581 gtgttcgaca tccagtttta agacccagct ttcttgtaca aagttggcat tataagaaag
     2641 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     2701 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2761 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2821 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2881 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2941 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     3001 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     3061 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     3121 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     3181 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     3241 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     3301 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     3361 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     3421 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     3481 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     3541 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     3601 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     3661 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     3721 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3961 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     4621 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4681 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     4741 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4801 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     4861 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4921 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4981 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     5041 gggccc
//
