LOCUS       sequence-352651-        3741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35A2_STOP
FEATURES             Location/Qualifiers
     source          1..3741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1298..1397)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1414..1433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1415..1433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1438..1454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1438..1454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1556..1575)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1567..2376
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1634..1653)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2523..3111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3012..3031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3265..3282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3441..3468)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3560..3646)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3695..3717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3709..3726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3710..3726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cgccgtggga
      121 gcaggaggaa gcaccgcagc accaggccct ggcgccgtgt ccgccggcgc cctggagcct
      181 ggaacagcat ctgccgcaca ccggagactg aagtacatca gcctggccgt gctggtggtg
      241 cagaacgcct ctctgatcct gagcatcaga tatgcccgca ccctgccagg cgacaggttc
      301 tttgccacca cagccgtggt catggccgag gtgctgaagg gcctgacatg cctgctgctg
      361 ctgtttgccc agaagcgcgg caatgtgaag cacctggtgc tgttcctgca cgaggccgtg
      421 ctggtgcagt acgtggatac cctgaagctg gccgtgccat ccctgatcta cacactgcag
      481 aacaatctgc agtatgtggc catctctaac ctgcccgccg ccacctttca ggtgacatat
      541 cagctgaaga tcctgaccac agccctgttc agcgtgctga tgctgaatcg gagcctgtcc
      601 agactgcagt gggcctccct gctgctgctg tttaccggcg tggccatcgt gcaggcacag
      661 caggcaggag gaggaggacc acggcccctg gaccagaacc ccggcgccgg cctggccgcc
      721 gtggtggcct cttgtctgag ctccggcttt gccggcgtgt acttcgagaa gatcctgaag
      781 ggctctagcg gctccgtgtg gctgcggaat ctgcagctgg gcctgttcgg aaccgccctg
      841 ggactggtgg gactgtggtg ggccgagggc accgccgtgg ccacaagagg cttctttttc
      901 ggatacacac cagccgtgtg gggagtggtg ctgaaccagg cctttggcgg actgctggtg
      961 gcagtggtgg tgaagtatgc cgacaatatc ctgaagggct tcgccacctc tctgagcatc
     1021 gtgctgtcca cagtggcctc tatcaggctg tttggcttcc acgtggatcc tctgtttgca
     1081 ctgggagcag gactggtcat cggagccgtg tacctgtata gcctgccaag gggagcagca
     1141 aaggcaatcg cctccgcctc tgccagcgcc tccggccctt gcgtgcacca gcagccccct
     1201 ggccagccac cccctccaca gctgtcctct cacagaggcg atctgatcac cgagcctttc
     1261 ctgccaaagc tgctgacaaa ggtgaagggc tcctaagacc cagctttctt gtacaaagtt
     1321 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1381 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1441 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1501 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1561 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1621 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1681 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1741 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1801 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1861 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1921 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1981 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2041 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2101 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2161 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2221 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2281 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2341 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2401 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2461 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3421 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3481 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3541 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3601 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3661 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3721 gccagtctta agctcgggcc c
//
