LOCUS       sequence-352665-       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human STAT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-STAT2
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3053..3641
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3106..3126)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3524..3535)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3644..3660)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3645..3661)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4166..4346
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4372..4389)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4378..4602
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4648..5076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4735..4754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4945..4966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5085..5105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5090..5419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5270..5405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5332..5351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5467..5514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5533..5907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6037..6158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6074..6093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6128..6147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6207..6223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6207..6223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6220..6242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6231..6247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6255..6261,6262..6279,6280..6285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6300..6321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6438..6455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6609..7197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6689..6708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7368..8159,8160..8228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7991..8010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8229..8333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8408..8427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8599..8978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8980..9178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9196..9376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9423..9548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10041..10274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10459..10503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10801..10918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatggcgca
      481 gtgggaaatg ctgcagaatc ttgacagccc ctttcaggat cagctgcacc agctttactc
      541 gcacagcctc ctgcctgtgg acattcgaca gtacttggct gtctggattg aagaccagaa
      601 ctggcaggaa gctgcacttg ggagtgatga ttccaaggct accatgctat tcttccactt
      661 cttggatcag ctgaactatg agtgtggccg ttgcagccag gacccagagt ccttgttgct
      721 gcagcacaat ttgcggaaat tctgccggga cattcagccc ttttcccagg atcctaccca
      781 gttggctgag atgatcttta acctccttct ggaagaaaaa agaattttga tccaggctca
      841 gagggcccaa ttggaacaag gagagccagt tctcgaaaca cctgtggaga gccagcaaca
      901 tgagattgaa tcccggatcc tggatttaag ggctatgatg gagaagctgg taaaatccat
      961 cagccaactg aaagaccagc aggatgtctt ctgcttccga tataagatcc aggccaaagg
     1021 gaagacaccc tctctggacc cccatcagac caaagagcag aagattctgc aggaaactct
     1081 caatgaactg gacaaaagga gaaaggaggt gctggatgcc tccaaagcac tgctaggccg
     1141 attaactacc ctaatcgagc tactgctgcc aaagttggag gagtggaagg cccagcagca
     1201 aaaagcctgc atcagagctc ccattgacca cgggttggaa cagctggaga catggttcac
     1261 agctggagca aagctgttgt ttcacctgag gcagctgctg aaggagctga agggactgag
     1321 ttgcctggtt agctatcagg atgaccctct gaccaaaggg gtggacctac gcaacgccca
     1381 ggtcacagag ttgctacagc gtctgctcca cagagccttt gtggtagaaa cccagccctg
     1441 catgccccaa actccccatc gacccctcat cctcaagact ggcagcaagt tcaccgtccg
     1501 aacaaggctg ctggtgagac tccaggaagg caatgagtca ctgactgtgg aagtctccat
     1561 tgacaggaat cctcctcaat tacaaggctt ccggaagttc aacattctga cttcaaacca
     1621 gaaaactttg acccccgaga aggggcagag tcagggtttg atttgggact ttggttacct
     1681 gactctggtg gagcaacgtt caggtggttc aggaaagggc agcaataagg ggccactagg
     1741 tgtgacagag gaactgcaca tcatcagctt cacggtcaaa tatacctacc agggtctgaa
     1801 gcaggagctg aaaacggaca ccctccctgt ggtgattatt tccaacatga accagctctc
     1861 aattgcctgg gcttcagttc tctggttcaa tttgctcagc ccaaaccttc agaaccagca
     1921 gttcttctcc aaccccccca aggctccctg gagcttgctg ggccctgctc tcagttggca
     1981 gttctcctcc tatgttggcc gaggcctcaa ctcagaccag ctgagcatgc tgagaaacaa
     2041 gctgttcggg cagaactgta ggactgagga tccattattg tcctgggctg acttcactaa
     2101 gcgagagagc cctcctggca agttaccatt ctggacatgg ctggacaaaa ttctggagtt
     2161 agtacatgac cacctgaagg atctctggaa tgatggacgc atcatgggct ttgtgagtcg
     2221 gagccaggag cgccggctgc tgaagaagac catgtctggc acctttctac tgcgcttcag
     2281 tgaatcgtca gaagggggca ttacctgctc ctgggtggag caccaggatg atgacaaggt
     2341 gctcatctac tctgtgcaac cgtacacgaa ggaggtgctg cagtcactcc cgctgactga
     2401 aatcatccgc cattaccagt tgctcactga ggagaatata cctgaaaacc cactgcgctt
     2461 cctctatccc cgaatccccc gggatgaagc ttttgggtgc tactaccagg agaaagttaa
     2521 tctccaggaa cggaggaaat acctgaaaca caggctcatt gtggtctcta atagacaggt
     2581 ggatgaactg caacaaccgc tggagcttaa gccagagcca gagctggagt cattagagct
     2641 ggaactaggg ctggtgccag agccagagct cagcctggac ttagagccac tgctgaaggc
     2701 agggctggat ctggggccag agctagagtc tgtgctggag tccactctgg agcctgtgat
     2761 agagcccaca ctatgcatgg tatcacaaac agtgccagag ccagaccaag gacctgtatc
     2821 acagccagtg ccagagccag atttgccctg tgatctgaga catttgaaca ctgagccaat
     2881 ggaaatcttc agaaactgtg taaagattga agaaatcatg ccgaatggtg acccactgtt
     2941 ggctggccag aacaccgtgg atgaggttta cgtctcccgc cccagccact tctacactga
     3001 tggacccttg atgccttctg acttctagtt cgaagatatc aagcttatcg ataatcaacc
     3061 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3121 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3181 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3241 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3301 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3361 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3421 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3481 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3541 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3601 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     3661 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     3721 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     3781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3841 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     3901 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     3961 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     4021 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     4081 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     4141 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     4201 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4261 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4321 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     4381 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4441 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4501 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4561 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4621 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5041 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5101 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5161 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5221 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5281 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5341 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     5401 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     5461 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     5521 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     5581 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     5641 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     5701 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     5761 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     5821 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     5881 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     5941 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6001 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6181 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6241 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6301 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6361 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6421 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6481 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8341 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8401 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8461 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8521 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     8581 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8641 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8701 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8761 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8821 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8881 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8941 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     9001 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     9061 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     9121 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     9181 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9241 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9301 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9361 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9421 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9481 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9541 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9601 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9661 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9721 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9781 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9841 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     9901 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     9961 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    10021 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    10081 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    10141 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    10201 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    10261 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    10321 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    10381 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    10441 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    10501 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    10561 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    10621 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    10681 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    10741 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
    10801 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
    10861 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
    10921 ggtttattac agggacagca gagatccagt ttggt
//
