LOCUS       sequence-352666-        8993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human TFEC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-TFEC
FEATURES             Location/Qualifiers
     source          1..8993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1091..1679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1144..1164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1562..1573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1682..1698)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1683..1699)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2204..2384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2410..2427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2416..2640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2686..3114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2773..2792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2983..3004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3123..3143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3128..3457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3308..3443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3370..3389
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3505..3552
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3571..3945
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4075..4196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4112..4131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4166..4185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4245..4261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4245..4261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4258..4280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4269..4285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4293..4299,4300..4317,4318..4323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4338..4359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4476..4493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4647..5235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4727..4746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5406..6197,6198..6266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6029..6048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6267..6371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6446..6465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6637..7016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7018..7216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7234..7414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7461..7586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8079..8312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8497..8541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8839..8956
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatgaccct
      481 tgatcatcag atcatcaatc caactcttaa atggtcacaa cctgcagtgc caagtggtgg
      541 gcctcttgtg cagcatgcac acacaactct ggacagtgat gctggcctca cagaaaaccc
      601 actcaccaag ttactagcta ttgggaaaga agatgacaat gcacaatggc atttatctgg
      661 aagtattttg gatgtgtata gcggtgaaca aggaatttca ccaattaaca tggggcttac
      721 aagtgcttct tgtccaagta gtctaccaat gaaaagagaa attacagaaa ctgacactag
      781 agctttagca aaagagagac aaaaaaagga caaccacaac ctcattgaaa gaagaagaag
      841 gtataatatt aattaccgaa tcaaggagct tggcactctt attccaaagt ctaatgatcc
      901 tgatatgcgc tggaacaaag gaaccattct aaaagcatca gtggagtaca tcaagtggct
      961 acaaaaagaa caacagagag cccgagaatt ggaacacaga cagaagaaat tagagcaggc
     1021 taacaggcga cttctacttc gaattcaggt ttttataaga atgtagttcg aagatatcaa
     1081 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1141 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1201 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     1261 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     1321 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     1381 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     1441 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     1501 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     1561 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     1621 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     1681 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     1741 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     1801 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1861 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     1921 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     1981 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2041 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     2101 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     2161 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     2221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     2401 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2461 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2521 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2581 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2641 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     2701 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2761 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2821 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2881 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2941 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3001 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3061 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3361 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     3421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     3481 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     3541 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     3601 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     3661 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     3721 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     3781 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     3841 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     3901 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     3961 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4021 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     4081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4201 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     4261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6001 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     6421 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6481 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     6541 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     6601 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     6661 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     6721 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6781 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6841 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6901 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6961 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7021 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7081 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7141 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7201 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     7261 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7321 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7381 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7441 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7501 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7561 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7621 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7681 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7741 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7801 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7861 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7921 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7981 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8041 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8101 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8161 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8221 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8281 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8341 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8401 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8461 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8521 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8581 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8641 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8701 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     8761 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     8821 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     8881 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     8941 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggt
//
