LOCUS       sequence-352668-       10049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human SP110 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-SP110
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2147..2735
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2200..2220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2618..2629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2738..2754)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2739..2755)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3260..3440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3466..3483)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3472..3696
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3742..4170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3829..3848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4039..4060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4179..4199)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4184..4513
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4364..4499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4426..4445
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4561..4608
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4627..5001
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5131..5252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5168..5187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5222..5241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5301..5317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5301..5317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5314..5336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5325..5341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5349..5355,5356..5373,5374..5379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5394..5415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5532..5549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5703..6291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5783..5802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6462..7253,7254..7322))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7085..7104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7323..7427)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7502..7521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7693..8072
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8074..8272
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8290..8470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8517..8642
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9135..9368
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9553..9597
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9895..10012
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gagctagcat
      481 gttcaccatg acaagagcca tggaagaggc tctttttcag cacttcatgc accagaagct
      541 ggggatcgcc tatgccatac acaagccatt tcccttcttt gaaggcctcc tagacaactc
      601 catcatcact aagagaatgt acatggaatc tctggaagcc tgtagaaatt tgatccctgt
      661 atccagagtg gtgcacaaca ttctcaccca actggagagg acttttaacc tgtctcttct
      721 ggtgacattg ttcagtcaaa ttaacctgcg tgaatatccc aatctggtga cgatttacag
      781 aagcttcaaa cgtgttggtg cttcctatga acggcagagc agagacacac caatcctact
      841 tgaagcccca actggcctag cagaaggaag ctccctccat accccactgg cgctgccccc
      901 accacaaccc cctcaaccaa gctgttcacc ctgtgcgcca agagtcagtg agcctggaac
      961 atcctcccag caaagcgatg agatcctgag tgagtcgccc agcccatctg accctgtcct
     1021 gcctctccct gcactcatcc aggaaggaag aagcacttca gtgaccaatg acaagttaac
     1081 atccaaaatg aatgcggaag aagactcaga agagatgccc agcctcctca ctagcactgt
     1141 gcaagtggcc agtgacaacc tgatccccca aataagagat aaagaagacc ctcaagagat
     1201 gccccactct cccttgggct ctatgccaga gataagagat aattctccag aaccaaatga
     1261 cccagaagag ccccaggagg tgtccagcac accttcagac aagaaaggaa agaaaagaaa
     1321 aagatgtatc tggtcaactc caaaaaggag acataagaaa aaaagcctcc caagagcctt
     1381 atctagacac ggaatccaaa agaagctcaa aagggtggat caggttcctc aaaagaaaga
     1441 tgactcaact tgtaactcca cggtagagac aagggcccaa aaggcgagaa ctgaatgtgc
     1501 ccgaaagtcg agatcagagg agatcattga tggcacttca gaaatgaatg aaggaaagag
     1561 gtcccagaag acgcctagta caccacgaag ggtcacacaa ggggcagcct cacctgggca
     1621 tggcatccaa gagaagctcc aagtggtgga taaggtgact caaaggaaag acgactcaac
     1681 ctggaactca gaggtcatga tgagggtcca aaaggcaaga actaaatgtg cccgaaagtc
     1741 cagatcgaaa gaaaagaaaa aggagaaaga tatctgttca agctcaaaaa ggagatttca
     1801 gaaaaatatt caccgaagag gaaaacccaa aagtgacact gtggattttc actgttctaa
     1861 gctccccgtg acctgtggtg aggcgaaagg gattttatat aagaagaaaa tgaaacacgg
     1921 atcctcagtg aagtgcattc ggaatgagga tggaacttgg ttaacaccaa atgaatttga
     1981 agtcgaagga aaaggaagga acgcaaagaa ctggaaacgg aatatacgtt gtgaaggaat
     2041 gaccctagga gagctgctga agagtggact tttgctctgt cctccaagaa taaatctcaa
     2101 gagagagtta aatagcaagt gattcgaaga tatcaagctt atcgataatc aacctctgga
     2161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2521 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2701 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     2761 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     2821 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     2881 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     2941 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     3001 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     3061 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     3121 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3181 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     3241 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3421 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     3481 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3541 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3601 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3661 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3721 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3961 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4021 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4081 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4141 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4201 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4261 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4321 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4381 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4441 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4501 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     4561 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     4621 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     4681 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     4741 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     4801 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     4861 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     4921 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     4981 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     5041 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5101 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     5161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     5281 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5341 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5401 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5461 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7441 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     7501 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     7561 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     7621 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     7681 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     7741 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7801 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7861 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7921 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7981 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8041 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8101 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     8161 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8221 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     8281 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8341 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8401 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8461 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8521 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8581 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8641 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8701 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8761 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8821 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8881 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8941 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     9001 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9061 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9121 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9181 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9241 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9301 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9361 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9421 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9481 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9541 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     9601 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     9661 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9721 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     9781 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     9841 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     9901 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9961 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
    10021 ttacagggac agcagagatc cagtttggt
//
