LOCUS       sequence-352670-        8885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human LITAF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-LITAF
FEATURES             Location/Qualifiers
     source          1..8885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    983..1571
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1036..1056)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1454..1465)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1574..1590)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1575..1591)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2096..2276
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2302..2319)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2308..2532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2578..3006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2665..2684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2875..2896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3015..3035)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3020..3349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3200..3335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3262..3281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3397..3444
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3463..3837
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3967..4088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4004..4023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4058..4077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4137..4153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4137..4153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4150..4172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4161..4177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4185..4191,4192..4209,4210..4215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4230..4251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4368..4385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4539..5127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4619..4638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5298..6089,6090..6158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5921..5940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6159..6263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6338..6357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6529..6908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6910..7108
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7126..7306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7353..7478
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7971..8204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8389..8433
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8731..8848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatgtcggt
      481 tccaggacct taccaggcgg ccactgggcc ttcctcagca ccatccgcac ctccatccta
      541 tgaagagaca gtggctgtta acagttatta ccccacacct ccagctccca tgcctgggcc
      601 aactacgggg cttgtgacgg ggcctgatgg gaagggcatg aatcctcctt cgtattatac
      661 ccagccagcg cccatcccca ataacaatcc aattaccgtg cagacggtct acgtgcagca
      721 ccccatcacc tttttggacc gccctatcca aatgtgttgt ccttcctgca acaagatgat
      781 cgtgagtcag ctgtcctata acgccggtgc tctgacctgg ctgtcctgcg ggagcctgtg
      841 cctgctgggg tgcatagcgg gctgctgctt catccccttc tgcgtggatg ccctgcagga
      901 cgtggaccat tactgtccca actgcagagc tctcctgggc acctacaagc gtttgtagtt
      961 cgaagatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     1021 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1081 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1141 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1201 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1261 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1321 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     1381 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     1441 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     1501 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     1561 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     1621 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     1681 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     1741 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     1801 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     1861 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     1921 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     1981 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     2041 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     2101 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     2161 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     2221 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     2281 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2341 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2401 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2461 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2521 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     2581 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2641 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2701 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2761 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2821 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2881 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2941 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3001 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     3301 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     3361 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     3421 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     3481 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     3541 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     3601 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     3661 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     3721 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     3781 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     3841 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     3901 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     3961 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4081 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     4141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     6301 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     6361 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     6421 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     6481 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     6541 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     6601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     6661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     6721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     6781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     6961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     7021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     7081 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     7141 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7201 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7261 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7321 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7381 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7441 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7501 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7561 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7621 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7681 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7741 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     7801 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     7861 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     7921 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     7981 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8041 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8101 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8161 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8221 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8281 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8341 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8401 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8461 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8521 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8581 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     8641 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     8701 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     8761 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     8821 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     8881 ttggt
//
