LOCUS       sequence-352707-       14102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of C. elegans grh-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Grigolon G, Araldi E, Erni R, Wu JY, Thomas C, La Fortezza M, Laube 
            B, Pohlmann D, Stoffel M, Zarse K, Carreira EM, Ristow M, Fischer F
  TITLE     Grainyhead 1 acts as a drug-inducible conserved transcriptional 
            regulator linked to insulin signaling and lifespan.
  JOURNAL   Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4.
  PUBMED    35013237
REFERENCE   2  (bases 1 to 14102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-10"; volume: "13(1):107. doi"; pages: " 
            10.1038/s41467-021-27732-4"
COMMENT     Sequence Label: pdestMB14_grh-1p-grh-1-gfp
FEATURES             Location/Qualifiers
     source          1..14102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2208..2228
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     RBS             3381..3392
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    4179..4203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5894..5918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5965..5993)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6741..6761
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     7697..7713
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7698..7714
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9671..9682)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(13461..13480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13462..13480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13487..13504)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13487..13503)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13496..13518)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(13644..14099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13781..13802)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13993..14012
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtacatcaa ctttgtatag
     2221 aaaagttggc gaaatttcct tcaatgcaaa ctttttgaga catgtataga atgttttaat
     2281 ttatttaaat agaagtatcc cctttcataa aaattttaac gtctgttgct aactttcatg
     2341 acaacttatt tacattaatt tttgaaaata gaactttcta gaatcatctg gaaaacgtaa
     2401 aacttatatt ttcacttttt ggtattagca ttccaaattg aaatttccaa tacaactcta
     2461 gctgaagcaa ggaattcctc aggatggtct taatagccta tatgtagtat agtatgaatg
     2521 tttaattccg actaactaca ttaaaagtta ggttagtcac tttttcattt catacgcttg
     2581 aaataaatta tttggcataa cgcaagacat gcatactttt ataatcctca tgcaaaaatt
     2641 ctcagcctat tttttttcca cactataata tactatatgt atgtcataga attaatagta
     2701 gctacctttt gctatataat actataagtt accacatttc aaagtttgag atcccattac
     2761 ggtttgatct acaaaaaatg caggaatttt ttgcccaaaa aatgtgacgt cagcaccacg
     2821 ttcttaacca tgcgaaatca gttgagaact ctgcgtctca actcccgcat tttttgtaga
     2881 tctaggtaga tcaaaccgaa atgagacagc ctgacaccac gtgaatatat atttagactt
     2941 aggagtaaat gtgtccaatt caaaaccagt tttttgttgt taaatacttg taattttgac
     3001 tattctcaaa aaaaaaccat taagagaaaa tgttcatgga taccctcctc tttcaaaagt
     3061 acccataact ttccttcaaa gctacttaat tcctgaacat catcatcatc ttcttcatct
     3121 ttcaggagac acggctaccc tttcactttt caccgaattc tcttttctct ccttccccat
     3181 tattataaga ttcttataat attattatta ttatccattc ttcattttga aaaagatatc
     3241 gatcccattt atatgcctat gacgtggcac tgaagacaaa aaggtcacac tcttttaatg
     3301 taaattagga ataaaaaacg ggtttttttt cgctttgaat cgcggaattt gttcgctttt
     3361 aatgatgaca aagaataaga aaagaggaga aaaacgtgtt tttaggctcc ttgaaatttt
     3421 ataataaaca atgaattgga ctttaactat ttagaaacaa actaattcca gtttaaaaaa
     3481 aattaaattt tacttttctt ccaaaatgta aaatttcgaa aatcgataag ttaaaattat
     3541 tgattctata tattattatt gatttttctc gaatttatta attccaatta atagtgctcg
     3601 tcagaaacat atattccata aatgtttgat ttaaaaattt tgccaagtac tcgccgaaaa
     3661 aaatatattc cagaatcaaa atttgacaat ttttgacaat cggaaaccat ccaacaaaat
     3721 tcgataacat tttttaaaaa atcaataagt tttcaattat cgatttttcg ttattttctt
     3781 aatttagtag tactttccga aaaccacgta ttccagaatt gaagcttcag aaatctggtt
     3841 aatctgaagc ccacgtggtg ccaggctgtc ccatcacggt ttgatctaca aaaaatgcgg
     3901 gaattgttta cccaaaaaaa tgtgacgtca gcaccacgtt cataaccatg cgaatttagt
     3961 tgagaactct gcgtctcttt tccgcgcatt tttgtagatc tacgtagatc aaacaaaaat
     4021 gagacgctct ggcaccacgt gaaagccgag tcggaatttt ttttttgaaa aattaataaa
     4081 ttcccccaaa ataaattatc gatttttagc caattttcac acgtacttta tccttttccc
     4141 attaaaaatc aataatatat cattaattat cattccagac aagtttgtac aaaaaagcag
     4201 gctatgtcat tccaacttga ccaatcaacg tcagtaataa aaacggcgcc gccggggcgc
     4261 ctagaattca aaaacgagcc aatcgacatt gcaagctcca atattcaaga cctagcgaaa
     4321 atgcaaacat tagacggata ttaccaacag acatcactac aacttccaca atcacaacaa
     4381 tatcatcgga tgtatgaaaa tgtctacaat aaccagcaga ttttatatgc tcaaactcaa
     4441 aactttcaat atccctcaac attactcacg aacggcagtc tcgcacaaac cgaatggagg
     4501 ggccaacaaa tggacacgtc cgcctattca aatacaggat attcgaggag ctcgacgagc
     4561 caacaacctc agtacacgca gcaagtgact gaaaatgagc ggattgtgta taagccagtt
     4621 gatatgccat caccagtgga ttcgggaatt ggtggtgata ttagtatttt gaatccgaaa
     4681 gaggagtttt ttacaagcgc cgatggaggt agtatgctgg aaagaagtag tgaaaggaca
     4741 cttagtcatc gggattctcc gctggttatt cctaagttat ataacaatct cggtttccaa
     4801 tatgtgctcg aggctccaat atccacgtca gtacgacgag atgatgatcg gatgacatat
     4861 gtgaataaag gacaattcta cacagtttca ttagagtata caccggatct gaacaaatgt
     4921 ttgaagagtc agacggttaa gagtcaactg atggttgtat tccgagagga caagacgtac
     4981 gaggaggaga tcaaaacgtg gcaatcttgg cacgcgcggc aacacgtgtc aaagcagcga
     5041 atcctggaaa ttgacagtaa aaacagctcg ggaatgatcg gacaaatcga ggagatcggc
     5101 aacaacgcgg tgcagttcta ctggaatccg tcggatccat ctggagtaag gatttccatc
     5161 gctgttcaat gtctttcaac tgatttttcg actcaaaaag gtgtcaaggg cctaccgctt
     5221 cacgtacaaa tcgatactta tgacggtgaa aatgacaaag tcccattcca caggggttac
     5281 tgtcagatta aagttttctg tgataaggga gctaatcgaa aaatgctgca cgaaaatcga
     5341 cgggatgaga agagacggca gcaagaatac acagcaggag cactacccgg tggcaggaag
     5401 aagtccgacg gtgaatacca tgatcagtgc gagcgcagcg aattttacca tatgcgagaa
     5461 ctcgacaagc ccgctgctct ttttattgct ccagaagaat ttgagcctcg ctacgtggat
     5521 agtacgagtc tgagctttga tatgtccgaa atcgagccga tacccacgaa aaggcccagg
     5581 acttcggaga ggataatgct ctacgttcga aaacgagacg agcaaattta ccagccgtta
     5641 cacgtggtac ccgcttcgct ttccggatta gcactcgcca tagccaacaa gttcggagcc
     5701 gacccggata agatgtccgg cgtctacaaa cgatgtgcaa aaggcatcac cgtaaaagtt
     5761 gacgacgaaa tgctccgact ctactgcaac gaggacacct tcataatcga tgtggagcac
     5821 gcgacggacg gctcaacagc cgccacgtta atcgaggtgg ctcccaccaa tccaaactcg
     5881 tattccaact catgcccagc tttcttgtac aaagttgatg taccggtaga aaaaatgagt
     5941 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     6001 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     6061 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     6121 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt
     6181 tatggtgttc aatgcttctc gagataccca gatcatatga aacggcatga ctttttcaag
     6241 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     6301 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     6361 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     6421 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     6481 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg
     6541 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     6601 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     6661 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     6721 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     6781 ggcatggatg aactatacaa atagcattcg tagaattcca actgagcgcc ggtcgctacc
     6841 attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa
     6901 ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct
     6961 gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     7021 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     7081 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     7141 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     7201 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
     7261 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     7321 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
     7381 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
     7441 tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta
     7501 aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg
     7561 ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt
     7621 tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg
     7681 ccgtacgggc cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat
     7741 tttatagttt tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca
     7801 tcactaatgc aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct
     7861 tatcattgct ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc
     7921 tctcaaaatt cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga
     7981 aatccttata tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa
     8041 atttccggca aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg
     8101 taattgccga aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt
     8161 ggaaatttca gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta
     8221 catctatttt gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat
     8281 ttcaaaaagg cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg
     8341 gcaaattgat atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac
     8401 aaatcggcaa accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt
     8461 tccagaggtt caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg
     8521 aattttggca gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag
     8581 ataaaatcct aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact
     8641 tcaaattgct ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc
     8701 tatttcatca caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc
     8761 tttgctcatc atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac
     8821 tctctcgctt ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc
     8881 attttccatc tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg
     8941 agcaagagca cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt
     9001 taaaaggtta ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg
     9061 aaagttttcc ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc
     9121 caaaaatttt gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt
     9181 taatcaatta attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt
     9241 tactcataaa aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa
     9301 actgtaaatc ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt
     9361 aaaaacacat tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa
     9421 tatttcacca attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct
     9481 caaaaaatat tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa
     9541 aattttctca aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag
     9601 aaaaaactgg tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt
     9661 acaaaaaaat ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa
     9721 tttttatttt aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag
     9781 aataacccta tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca
     9841 ccacctctaa tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac
     9901 aacagcatat atgaaggcag atctacaaca acaatcgatc gcacccggct cggcaacctt
     9961 cccgtctcag gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt
    10021 atactaactc ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt
    10081 tgaaattgtt tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca
    10141 aataaattta atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat
    10201 gaccgaataa cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca
    10261 aatttcgcta gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc
    10321 aatcaggggt ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac
    10381 aaaaaatgcg ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg
    10441 ggtgttcggc aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc
    10501 cttttatgaa cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat
    10561 atcaaaccgt gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg
    10621 acaaatcggc aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc
    10681 cgaaaaaaaa tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct
    10741 aaaaatttcc ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc
    10801 tgcaatttgc cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt
    10861 tgaatattaa aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc
    10921 tatgtacaaa aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt
    10981 tgccgaacat aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat
    11041 gccccgaccc ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa
    11101 aaatcaaatt acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat
    11161 atttgctcta ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag
    11221 gattcctatg ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc
    11281 gtgacctcga cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag
    11341 agaatctgca ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt
    11401 ttctgaaatt aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca
    11461 cacattttcg aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt
    11521 ttgaatttgg attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc
    11581 cacaactttc acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg
    11641 tcaatagagc gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc
    11701 tacagtagtt caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg
    11761 gcgggtaatt caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa
    11821 aattatttga cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt
    11881 taaaaactac attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca
    11941 tcgaagagaa gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa
    12001 actgactaat tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa
    12061 aattaaaaaa aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc
    12121 cgctccgata gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac
    12181 tcgtatgatg cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat
    12241 agaaatcatg tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc
    12301 tcacaaaatg cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat
    12361 gatttttcaa atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat
    12421 aaaattaaaa aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca
    12481 taaaaattaa atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc
    12541 aactaaccga tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga
    12601 tttgccggaa atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac
    12661 tttagataaa acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg
    12721 aagttttcag ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa
    12781 aactgccgat ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat
    12841 ttgccgaaca ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa
    12901 ctaacggatt tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga
    12961 ctaatcatgt tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac
    13021 atcttcttca tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg
    13081 ttatcagtag gaaaagcctc cttttatctc attatcagca attgccttct tcttcttctt
    13141 ctttgaatac catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag
    13201 caggaggaaa agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa
    13261 aattttccga catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc
    13321 ccactcattt gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt
    13381 ttgttatgtt cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac
    13441 cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
    13501 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    13561 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    13621 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt
    13681 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
    13741 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    13801 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    13861 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    13921 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    13981 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
    14041 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
    14101 ag
//
