LOCUS       sequence-352710-       12499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of C. elegans grh-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Grigolon G, Araldi E, Erni R, Wu JY, Thomas C, La Fortezza M, Laube 
            B, Pohlmann D, Stoffel M, Zarse K, Carreira EM, Ristow M, Fischer F
  TITLE     Grainyhead 1 acts as a drug-inducible conserved transcriptional 
            regulator linked to insulin signaling and lifespan.
  JOURNAL   Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4.
  PUBMED    35013237
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-10"; volume: "13(1):107. doi"; pages: " 
            10.1038/s41467-021-27732-4"
COMMENT     Sequence Label: pdestMB14_hsp-16.2p-grh-1-gfp
FEATURES             Location/Qualifiers
     source          1..12499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2208..2228
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2576..2600
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4291..4315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4362..4390)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5138..5158
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     6094..6110
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6095..6111
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8068..8079)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(11858..11877)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11859..11877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11884..11901)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11884..11900)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11893..11915)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12041..12496)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12178..12199)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12390..12409
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtacatcaa ctttgtatag
     2221 aaaagttgat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga
     2281 gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag
     2341 atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct
     2401 gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg
     2461 caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat
     2521 aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact ataatccaag
     2581 tttgtacaaa aaagcaggct atgtcattcc aacttgacca atcaacgtca gtaataaaaa
     2641 cggcgccgcc ggggcgccta gaattcaaaa acgagccaat cgacattgca agctccaata
     2701 ttcaagacct agcgaaaatg caaacattag acggatatta ccaacagaca tcactacaac
     2761 ttccacaatc acaacaatat catcggatgt atgaaaatgt ctacaataac cagcagattt
     2821 tatatgctca aactcaaaac tttcaatatc cctcaacatt actcacgaac ggcagtctcg
     2881 cacaaaccga atggaggggc caacaaatgg acacgtccgc ctattcaaat acaggatatt
     2941 cgaggagctc gacgagccaa caacctcagt acacgcagca agtgactgaa aatgagcgga
     3001 ttgtgtataa gccagttgat atgccatcac cagtggattc gggaattggt ggtgatatta
     3061 gtattttgaa tccgaaagag gagtttttta caagcgccga tggaggtagt atgctggaaa
     3121 gaagtagtga aaggacactt agtcatcggg attctccgct ggttattcct aagttatata
     3181 acaatctcgg tttccaatat gtgctcgagg ctccaatatc cacgtcagta cgacgagatg
     3241 atgatcggat gacatatgtg aataaaggac aattctacac agtttcatta gagtatacac
     3301 cggatctgaa caaatgtttg aagagtcaga cggttaagag tcaactgatg gttgtattcc
     3361 gagaggacaa gacgtacgag gaggagatca aaacgtggca atcttggcac gcgcggcaac
     3421 acgtgtcaaa gcagcgaatc ctggaaattg acagtaaaaa cagctcggga atgatcggac
     3481 aaatcgagga gatcggcaac aacgcggtgc agttctactg gaatccgtcg gatccatctg
     3541 gagtaaggat ttccatcgct gttcaatgtc tttcaactga tttttcgact caaaaaggtg
     3601 tcaagggcct accgcttcac gtacaaatcg atacttatga cggtgaaaat gacaaagtcc
     3661 cattccacag gggttactgt cagattaaag ttttctgtga taagggagct aatcgaaaaa
     3721 tgctgcacga aaatcgacgg gatgagaaga gacggcagca agaatacaca gcaggagcac
     3781 tacccggtgg caggaagaag tccgacggtg aataccatga tcagtgcgag cgcagcgaat
     3841 tttaccatat gcgagaactc gacaagcccg ctgctctttt tattgctcca gaagaatttg
     3901 agcctcgcta cgtggatagt acgagtctga gctttgatat gtccgaaatc gagccgatac
     3961 ccacgaaaag gcccaggact tcggagagga taatgctcta cgttcgaaaa cgagacgagc
     4021 aaatttacca gccgttacac gtggtacccg cttcgctttc cggattagca ctcgccatag
     4081 ccaacaagtt cggagccgac ccggataaga tgtccggcgt ctacaaacga tgtgcaaaag
     4141 gcatcaccgt aaaagttgac gacgaaatgc tccgactcta ctgcaacgag gacaccttca
     4201 taatcgatgt ggagcacgcg acggacggct caacagccgc cacgttaatc gaggtggctc
     4261 ccaccaatcc aaactcgtat tccaactcat gcccagcttt cttgtacaaa gttgatgtac
     4321 cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     4381 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     4441 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     4501 gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagccaacac
     4561 ttgtcactac tttctgttat ggtgttcaat gcttctcgag atacccagat catatgaaac
     4621 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     4681 tcaaagatga cgggaactac aagacacgta agtttaaaca gttcggtact aactaaccat
     4741 acatatttaa attttcaggt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     4801 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     4861 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     4921 ttgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagaacttca
     4981 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     5041 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     5101 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     5161 ctgctgggat tacacatggc atggatgaac tatacaaata gcattcgtag aattccaact
     5221 gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc
     5281 atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat
     5341 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     5401 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     5461 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     5521 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     5581 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     5641 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     5701 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     5761 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     5821 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     5881 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     5941 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     6001 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     6061 tgtttcctac tagtcggccg tacgggcccc ccctcgaggt cgacggtatc gataagcttc
     6121 agtaaaagaa gtagaatttt atagtttttt ttctgtttga aaaattctcc ccatcaatgt
     6181 tctttcaaat aaatacatca ctaatgcaaa gtattctata acctcatatc taaattcttc
     6241 aaaatcttaa catatcttat cattgcttta agtcaaacgt aacattaaaa aaaatgtttt
     6301 ggaaaatgtg tcaagtctct caaaattcag ttttttaaac cactcctata gtcctatagt
     6361 cctatagtta cccatgaaat ccttatatat tactgtaaaa tgtttcaaaa accattggca
     6421 aattgccaga actgaaaatt tccggcaaat tggggaaccg gcaaattgcc aatttgctga
     6481 atttgccgga aaaacggtaa ttgccgaaag tttttgacac gaaaatggca aattgtggtt
     6541 ttaaaatttt tttttttgga aatttcagaa tttcaatttt aatcggcaaa actgtaggca
     6601 tcctaagaat gttcctacat ctattttgaa aagtaagcga attctatgaa aatgtctaaa
     6661 gaaaatgggg aaacaatttc aaaaaggcac agtttcaagt gtttccgaat tatactaaat
     6721 ccctctaaaa acttccggca aattgatatc cgtaaaagag caaatccgca tttttgccga
     6781 aaattaaaat ttccgacaaa tcggcaaacc ggcaatttgg cgaaatttgc cggaaggcaa
     6841 ttgccgccca cccctgttcc agaggttcaa actggtagca aagctcaaaa tttctcaaat
     6901 tctccaattt ttttttgaat tttggcagtg taccaaaatg acattcagtc atattggttt
     6961 attatagatt tatttagata aaatcctaaa tgattctacc tttaaagatg cccactttaa
     7021 aagtaatgac tcaaacttca aattgctcta agattctatt gaattaccat cttttcctct
     7081 cattttctct cactgtctat ttcatcacaa attcatccct ctctccctct ctttctctct
     7141 ccctctctct ctttctcttt gctcatcatc tgtcattttg tccgttcctc tctctgcgcc
     7201 ctcagcgttc cccacactct ctcgcttctc ttttcctaga cgtcttcttt tttcatcttc
     7261 ttcagccctt tttcgccatt ttccatctct gtcaatcatt acggacgacc cccattaatt
     7321 tttggatgtg ctctgcgagc aagagcacac ggaactcatt tattttttgt tggaatttct
     7381 ttttcatttt agttagttaa aaggttattc agtgcaaaaa ttgatttttt gctcttttgg
     7441 tacattagag gggtttgaaa gttttccata aaaagggttt ccggagtaaa aaatctaaaa
     7501 atttttacaa acatttccaa aaattttggg aacatatctt ttcaaaaact caaaaaaaaa
     7561 cgcaaacatc ataaatttaa tcaattaatt ggatgcgttc aaatatttta aaaatatttt
     7621 tgaattgcat atttctttac tcataaaaaa attattaatg tttaagtaaa aaaaatattt
     7681 caggatttta aacaaaaact gtaaatccca taaaacagtg ttatttttta gttattttta
     7741 gtaattattt gtgaattaaa aacacatttt ttttcaaaat attccttgaa taaataaaat
     7801 tgaaagtgaa ttcaaaatat ttcaccaatt ttacaaaatt tgaaaatttg aaaaaatatt
     7861 ttctaccaaa ttgtgctcaa aaaatattat taagttccca ggaaatttat gatgaaaact
     7921 tggaatattt ttggtaaaat tttctcaaaa aattgaaagt tgtcaaaaac acactaacac
     7981 tatacctcgc ggcatagaaa aaactggtgg ccgaattttt taaaccaatt taaattaaat
     8041 atatttttgc actttgtaca aaaaaatttg aagttcccag gaaattcata cggaaatatt
     8101 ttccagaact tttataattt ttattttaat tctcaaaatt tacaataaaa acttggaaac
     8161 ctgaaatttg cttaaagaat aaccctatcc cccacggcct agaaaatact ggtggccgaa
     8221 ttttttccgc agccacacca cctctaatct ccagagcact ctccaaaatt cccataatcc
     8281 ccaaaatttt cagctacaac agcatatatg aaggcagatc tacaacaaca atcgatcgca
     8341 cccggctcgg caaccttccc gtctcaggtg aggaagacgc agagaaatga gagagagagc
     8401 gtgtttatgc acgcagtata ctaactcctg atatccaatt tcatatatcg atatgcttgc
     8461 tttcttttct tctacgttga aattgtttga acttttctca aaaaacgcgg attttagaga
     8521 gacttagagt tgctccaaat aaatttaatt tgcaattttg cacttactat tagaatctac
     8581 ttttatagcg ctaaaatgac cgaataacac agtagatttt aaaagaaaat tttaaaaatt
     8641 atttttttgt gcagtcaaat ttcgctagtt tttgccactc atttttgaac atttgtatat
     8701 gtgcctttcg gtggttcaat caggggtggc acgtggtgtc agagtgttcc atttcggttt
     8761 gatctacgtt gatctacaaa aaatgcggga gaagagacgc agagttctca actgatttca
     8821 catggttatg aaccaggggt gttcggcaaa tttgccgttt gccgagctgg gcaaattttg
     8881 agatttgccg cacacccctt ttatgaacgt gctgacgtca catctttttg gacaaagaat
     8941 tcccgcattt tttgtatatc aaaccgtgat gggacagcct ggcaccacgt ggggttggcg
     9001 gcaaatgatt ttttccgaca aatcggcaaa ttggcaaatt gccggaattt aaagtttctg
     9061 gcaaattgtt gatttgccga aaaaaaattt caaaaaggca cagttttaag tgtttccgtc
     9121 ttataaaata tttctctaaa aatttccgga aaacggaaaa ttgccggaaa tacaaagttt
     9181 cgtcaaatcg gcaaacctgc aatttgccga acggcaactg ccgccaacac ctggttccca
     9241 aaaaagcgtg cactgtttga atattaaaaa aaaaattcac acaaaaataa taaattgaaa
     9301 taggctaaat tcacatctat gtacaaaaat tgcatgccag caccggtcaa ttttctcaat
     9361 tactttttgt ctatttttgc cgaacataat ttgctgtttc cttggcagaa tacagaaaac
     9421 tctttttttt ttcagatgcc ccgaccccca ccggtaaccg aacaggctat aaccaccgag
     9481 gcggagcttc tcgcgaaaaa tcaaattaca ccaaatgatg tgctagctct tccaggaatc
     9541 actcaaggta agtgaatatt tgctctattg ataaacgcgc cgtgtactcc acgtggacaa
     9601 acatatcaaa ttttcaggat tcctatgctc cccatcggca aacgtctata acattgagtt
     9661 caccaaattc caaatccgtg acctcgacac ggaacacgtg ctcttcgaaa ttgcaaaacc
     9721 ggaaaatgag acggaagaga atctgcaggc gcaagccgaa tcggcaagat atgtccgata
     9781 tcgatttgcg ccgaattttc tgaaattaaa gacggtcggc gcgacggtcg agttcaaggt
     9841 cggcgatgtg ccgatcacac attttcgaat gattgaacgg cactttttca aggatcgttt
     9901 attgaaatgc tttgattttg aatttggatt ctgtatgcca aattcgagaa acaactgtga
     9961 acatatctat gaatttccac aactttcaca acaactcagt gagtttgaag cgggaaaaat
    10021 tatttgagct cattttgtca atagagcgaa ttttctaggt ttttaaaaat aaaataaata
    10081 ttgacaaaaa gctgccctac agtagttcaa aatgtgaaaa cttggaggaa attcgagttt
    10141 tctctgagaa gtttttggcg ggtaattcaa attttctgag aaaaaaatta gccgcgagaa
    10201 ttcaaatttt ctgagaaaat tatttgacgg gaaattcaaa atttctggaa atgtattgaa
    10261 catttgagaa attttgttaa aaactacatt aaaccgataa aaaagttcgc aaattttttt
    10321 taagtgctca tggtgcatcg aagagaagaa ttcttgccaa aactagccca tgaaattaaa
    10381 aattatcgcg gaaaaaaact gactaattgg aaagtaaaat gaaatttgca aaaaaaaaat
    10441 atgttttttt gttgtaaaat taaaaaaaaa actgtatatt ttcagtggac gacatgatca
    10501 ataatccaaa cgagacccgc tccgatagct tctattttgt ggagaataag ctcgtaatgc
    10561 acaataaggc cgactactcg tatgatgcat aatttcccgc cgatggaata aaactaataa
    10621 tttttctcgc aatttataga aatcatgtga ttttcctacc gctaaattta aaatttccta
    10681 atttttcact atatttctca caaaatgcat tccgaaaact ctcatttttc agcttttcaa
    10741 tacaaatccc aataaatgat ttttcaaata taatgttctc aattgatatg ttttatttga
    10801 atgttggaaa aaaccataaa attaaaaaaa acttgaatta ttcaaaaata attcggccct
    10861 tttacaaaaa attatcataa aaattaaatt tggaaaatct gtgatttgtc gctttgccgg
    10921 aagtttccaa ttccggcaac taaccgattt gccgattagc tagaaatttc aattccgaca
    10981 atgtgccgct ttgccgattt gccggaaatt tccaattccg gcaattaacg gacttgccga
    11041 ttagctagaa atttaacttt agataaaact tctcaacaaa atcaaattgg aaagtcagcg
    11101 atttgccgct ttgccggaag ttttcagttc cggcatctaa ccgatttgcc gatttgccaa
    11161 aaatgtcaat tccgaaaaac tgccgatttg ccgctttgtc ggaaatttca attccgacaa
    11221 attgccgatt tgccgatttg ccgaacattc caattccgac catttgtcga tttgccggaa
    11281 gttaccaatt ccggcaacta acggatttgc cgatttgccg gaaaaaaatt gtttgtcgcc
    11341 aaccctgatt taaatgacta atcatgttgc aaaagataac tgcttgcatc aaaaatccga
    11401 tcattggcat tcccaacatc ttcttcatcc aaaacaccac ccttttcttc cactaatcat
    11461 gaattcccga ccaattgtta tcagtaggaa aagcctcctt ttatctcatt atcagcaatt
    11521 gccttcttct tcttcttctt tgaataccat ttgaatggag atatcattta cataaatctg
    11581 ccgccgagat tgtgaagcag gaggaaaagc tgaagatact gatattatat gtatatttca
    11641 ttattctttt tccacaaaat tttccgacat ttaattccac ttcactttca tcgtctcttt
    11701 ttcccccatt gtcgccccca ctcatttgtc ttcttcttca aatacgggtc aatcactttc
    11761 cgttttttat tcattttttg ttatgttcgg cttaacaaat ttgaagtatt gttaaaattc
    11821 tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa
    11881 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
    11941 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
    12001 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
    12061 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
    12121 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    12181 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    12241 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    12301 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    12361 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    12421 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    12481 ccgctacagg gcgcgtcag
//
