LOCUS       sequence-35273-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: pCS2 GSK3-RFP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(147..173)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(214..232)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(215..232)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
       61 tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac
      121 tccttgatga cgtcctcgga ggaggctgcg tagtcgggga cgtcgtaggg gtacatggtg
      181 gcggatcctg caaaaagaac aagtagcttg tattctatag tgtcacctaa atcaagcttg
      241 ctccgacgtc cccaggcaga atggcggttc cctaaacgag cattgcttat atagacctcc
      301 cattaggcac gcctaccgcc catttacgtc aatggaacgc ccatttgcgt cattgcccct
      361 ccccattgac gtcaatgggg atgtacttgg cagccatcgc gggccattta ccgccattga
      421 cgtcaatggg agtactgcca atgtaccctg gcgtacttcc aatagtaatg tacttgccaa
      481 gttactatta atagatattg atgtactgcc aagtgggcca tttaccgtca ttgacgtcaa
      541 tagggggcgt gagaacggat atgaatgggc aatgagccat cccattgacg tcaatggtgg
      601 gtggtcctat tgacgtcaat gggcattgag ccaggcgggc catttaccgt aattgacgtc
      661 aatgggggag gcgccatata cgtcaatagg accgcccata tgacgtcaat aggaaagacc
      721 atatatagag accattgacg tcaatggggg agtggctatg ggcggtatta ggaagcccca
      781 tatatggtat atgggaccgc ccattgggag gggctatcta cgtcaatagg aaaacccata
      841 tatggaatac tatatggcat ag
//
