LOCUS       sequence-35274-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4178 (=HL 862 + pHL 1360)
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    171..189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(210..228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        296..400
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(619..638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttctccttta cgcatgctag ctgggaccag gagggacagt actttaactt tcatgttatt
       61 aaccctctgt tatatgcctt tatttgcttt tttatgccac tgcatactga ttaaccctca
      121 ttaatcagtc ggcaagtcga cagtatctct atcactgata gggatgtcaa tctctatcac
      181 tgatagggac tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      241 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      361 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      421 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      481 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      541 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      601 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      661 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      721 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      781 gagtgataac actgcggcca ac
//
